Fingerprint spectrum detection method of uncaria hirsuta
A fingerprint and detection method technology, applied in the direction of measuring devices, instruments, scientific instruments, etc., can solve problems such as differences in chemical composition, and achieve the effect of scientific harvesting, strong operability, and high stability
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Embodiment 1
[0085] The fingerprint detection method of Uncaria torticola comprises the following:
[0086] (1) Preparation of the test solution: get the uncaria to-be-tested sample powder containing 2.0 g of Uncaria, add 2 mL of ammonia water and soak for 30 min, then add 25 mL of chloroform, 5 ml of methanol, ultrasonically extract for 60 min, take out, put Cool, filter, evaporate the filtrate to dryness on a 60°C water bath, dissolve the residue in a 10mL measuring bottle with methanol and set the volume to the mark, shake well, filter with a 0.45μm microporous membrane, and take the subsequent filtrate as the test solution;
[0087] (2) Chromatographic conditions: The chromatographic column uses octadecylsilane bonded silica gel as the filler: mobile phase A is acetonitrile, mobile phase B is 0.2% ammonia solution, gradient elution, parameters: 0 ~ 45min, 72% ~ 45 %B; 45min~60min, 45%~37%B, the flow rate is 1.0ml / min, the detection wavelength is 254nm, the column temperature is 25℃, an...
Embodiment 2
[0095] The fingerprint detection method of Uncaria torticola comprises the following:
[0096] (1) Preparation of the test solution: get the uncaria to-be-tested sample powder containing 2.0 g of Uncaria, add 1 mL of ammonia water and soak for 40 min, then add 20 mL of chloroform, 8 ml of methanol, ultrasonically extract for 45 min, take out, put Cool, filter, evaporate the filtrate to dryness on a 50°C water bath, dissolve the residue in a 10mL measuring bottle with methanol and set the volume to the mark, shake well, filter through a 0.45μm microporous membrane, and take the subsequent filtrate as the test solution;
[0097] (2) Chromatographic conditions: The chromatographic column uses octadecylsilane bonded silica gel as the filler: the mobile phase A is acetonitrile, the mobile phase B is 0.1% ammonia solution, gradient elution, the parameters are: 0 ~ 45min, 72% ~ 45 %B; 45min~60min, 45%~37%B, flow rate 0.8ml / min, detection wavelength 260nm, column temperature 20°C, rec...
Embodiment 3
[0105] The fingerprint detection method of Uncaria torticola comprises the following:
[0106] (1) Preparation of the test solution: get the uncaria to-be-tested sample powder containing 2.0 g of Uncaria, add 3 mL of ammonia water and soak for 20 min, then add 30 mL of chloroform, 2 ml of methanol, ultrasonically extract for 75 min, take out, put Cool, filter, evaporate the filtrate to dryness on a 60°C water bath, dissolve the residue in a 10mL measuring bottle with methanol and set the volume to the mark, shake well, filter with a 0.45μm microporous membrane, and take the subsequent filtrate as the test solution;
[0107] (2) Chromatographic conditions: The chromatographic column uses octadecylsilane bonded silica gel as the filler: mobile phase A is acetonitrile, mobile phase B is 0.1%-0.3% ammonia solution, gradient elution, parameters: 0-45min, 72 %~45%B; 45min~60min, 45%~37%B, the flow rate is 1.2ml / min, the detection wavelength is 254nm, the column temperature is 30℃, a...
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