Method and device of continuously automatically reading transmission signals of multiple biochips

A biochip and chip technology, applied in the direction of electromagnetic radiation induction, instruments, induction recording carriers, etc., can solve the problems of signal distortion, low degree of automation, signal value interference, etc., to eliminate the interference of pollutants, improve accuracy and automation degree, the effect of improving accuracy

Active Publication Date: 2016-05-18
SHANGHAI BAIO TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Dust, fiber, background and other pollutants with a certain signal value sometimes exist in the area around the array point of the chromogenic microarray biochip, which will interfere with the coordinate positioning of the signal point and the accurate collection of signal value
The current chromogenic microarray biochip reader usually needs to manually confirm the type of the chip, and then manually adjust it in the computer software to find the correct array point, which has a low degree of automation and low efficiency; when multiple types of biochips need to be read When using biochips, the efficiency of manual reading will be lower, and there may be mistakes in manually confirming the chip type due to the increase in work intensity, which will lead to a decrease in the accuracy of chip reading results; the collected signal values ​​​​are often due to the above-mentioned chip Pollutants and manual operation lead to interference and distortion; in the chip signal collection link, usually the light passes through the chip and then shines on the backlight board, and the backlight board reflects the chip signal and then is collected, and the dust accumulated on the backlight board affects the signal value. The interference is very large, and it will also cause the collected signal to be distorted; one chip is detected at a time, and the detection throughput is low

Method used

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  • Method and device of continuously automatically reading transmission signals of multiple biochips
  • Method and device of continuously automatically reading transmission signals of multiple biochips
  • Method and device of continuously automatically reading transmission signals of multiple biochips

Examples

Experimental program
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Effect test

Embodiment 1

[0124] Put the color-developing microarray biochips to be tested into the chip groove of the loading tray 51 of the reader, 8 pieces can be put in at a time, and the loading tray 51 is driven by the motor 53 to move grid by grid. When the chip to be tested is directly below the CCD main camera 11 and the chip barcode is directly below the secondary camera 12, the movement stops. Using the sub-camera illumination light source 15, the sub-camera 12 takes pictures and samples the barcode area of ​​the chip. The chip detection image formed by the transmission of the main camera LED background light source 13 is taken and sampled by the CCD main camera 11 multiple times to obtain the chip image.

[0125] Next, the image processing unit first trims the original image of the chip, takes out the imaging part, and then normalizes the brightness of the image, then performs inversion and binarization on the image, and then performs erosion / dilation to remove noise.

[0126] Then, the si...

Embodiment 2

[0132] Except that the signal point identification and positioning unit 3 adopts the following scheme, the rest is the same as the embodiment 1.

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Abstract

The invention provides a method of continuously automatically reading transmission signal values of multiple developing microarray biochips, and a reading instrument and a reading system for realizing the method. The reading method is characterized by comprising: (1) scanning a glass chip bar code to obtain chip bar code information; (2) placing the main camera used for shooting a chip image and the background light source used for main camera lighting on two sides of a glass chip; (3) identifying parameters according to the bar code information, chip images and signal points, automatically identifying and positioning the signal points, and automatically obtaining signal point coordinates; (4) automatically obtaining signal values of the signal points according to the obtained signal point coordinates; and (5) automatically continuously reading a plurality of chips (which can be chips of different types) through position movement.

Description

technical field [0001] The present invention relates to a biochip reading method, a reading instrument and a reading system for realizing the method, in particular to a method for continuously and automatically reading multiple transmission signal values ​​of color-developing microarray biochips, A reading instrument and a reading system for realizing the method. Background technique [0002] Microarray biochips synthesize or immobilize a series of polymers, such as oligonucleotides, polypeptides, and antibodies, in a lattice arrangement on glass substrates, silicon substrates, or polymer material substrates. These polymers can be labeled with fluorescent or chemichromic markers for optical detection. Chromogenic microarray chips usually use chemical chromogenic markers such as digoxin, horseradish peroxidase, and biotin to mark the polymers immobilized on the chip, so that they can be read by a biochip reader. Response result information. The chromogenic microarray bioch...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G06K7/10
CPCG06K7/10821
Inventor 朱滨邢军芬林志松陆恩泽魏悦张莹
Owner SHANGHAI BAIO TECH
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