Application of system for detecting expression quantity of eight miRNAs in preparation of product for diagnosing or assisting in diagnosing hepatocellular carcinoma

A technology for auxiliary diagnosis of hepatocellular carcinoma, applied in the direction of microbial measurement/testing, biochemical equipment and methods, etc., can solve the problem of low sensitivity of liver cancer, achieve the effect of improving sensitivity, good prediction effect, and increasing the proportion of early diagnosis

Active Publication Date: 2016-05-25
CAPITALBIO CORP +1
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Problems solved by technology

At present, the tumor marker clinically used to diagnose liver cancer is mainly alpha-fetoprotein (AFP), but about 40% of liver cancers do not secrete AFP, making the sensitivity of liver cancer detection generally low (20%-65%)

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  • Application of system for detecting expression quantity of eight miRNAs in preparation of product for diagnosing or assisting in diagnosing hepatocellular carcinoma
  • Application of system for detecting expression quantity of eight miRNAs in preparation of product for diagnosing or assisting in diagnosing hepatocellular carcinoma
  • Application of system for detecting expression quantity of eight miRNAs in preparation of product for diagnosing or assisting in diagnosing hepatocellular carcinoma

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Embodiment 1、8

[0048] Example 1, 8 miRNAs can be used to diagnose hepatocellular carcinoma

[0049] 1. Sample selection

[0050] The inventor of the present application collected plasma samples that meet the standards according to the standard operating procedure (SOP), systematically collected complete demographic data, clinical data, etc., and through sorting out the sample data, the inventor selected the plasma samples of 877 people as TLDA (TaqmanLowDensityArray, TLDA) chip detection and subsequent series of qRT-PCR verification experimental samples, these 877 people include 272 cases of hepatocellular carcinoma patients in the hepatocellular carcinoma group, 275 healthy people in the healthy control group, and benign liver disease group. 155 patients with benign liver diseases and 175 patients with benign and malignant other tissues and organs (including 30 cases of lung cancer, 30 cases of gastric cancer, 30 cases of colorectal cancer, 21 cases of esophageal cancer, 29 cases of benign ...

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Abstract

The invention discloses application of a system for detecting the expression quantity of eight miRNAs in preparation of a product for diagnosing or assisting in diagnosing hepatocellular carcinoma. The hepatocellular carcinoma can be well diagnosed with a model built by the content of the eight RNAs including has-miR-20a, hsa-miR-21, hsa-miR-26a, hsa-miR-122, hsa-miR-192, hsa-miR-146a, hsa-miR-223 and hsa-miR-483-5 in blood plasma, the sensitivity can reach 69.6%, the specificity can reach 92.8%, the accuracy rate can reach 83.3%, and the sensitivity obtained when the eight miRNAs and AFP are utilized to perform joint diagnosis on the hepatocellular carcinoma can reach 85.7%. Therefore, the miRNAs in the blood plasma can diagnose the hepatocellular carcinoma by serving as a tumor diagnosis marker and also can diagnose the hepatocellular carcinoma together with the AFP.

Description

technical field [0001] The invention relates to the application of a system for detecting the expression levels of eight miRNAs in the preparation of products for diagnosis or auxiliary diagnosis of hepatocellular carcinoma in the field of biotechnology. Background technique [0002] Liver cancer refers to malignant tumors that occur in the liver, including primary liver cancer and metastatic liver cancer. People usually refer to primary liver cancer as liver cancer. Primary liver cancer can be divided into hepatocellular carcinoma (hepatocellular carcinoma, HCC), intrahepatic cholangiocarcinoma and mixed liver cancer according to pathological classification, and HCC liver cancer accounts for about 90% of primary liver cancer. Primary liver cancer is one of the most common malignant tumors in clinical practice. According to the latest statistics, there are about 600,000 new liver cancer patients in the world every year, ranking fifth among malignant tumors. At present, the ...

Claims

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Application Information

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IPC IPC(8): C12Q1/68
CPCC12Q1/6886C12Q2600/158C12Q2600/178
Inventor 郭弘妍杨春花董家鸿张爱群孙义民王亚辉邢婉丽程京
Owner CAPITALBIO CORP
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