Gibberellin fermentation liquid membrane extraction water crystallization process

Abstract

The invention discloses a gibberellin fermentation liquid membrane extraction water crystallization process. The process includes steps: S1, performing plate-and-frame filtering on gibberellin fermentation liquid, and enabling filtrate to enter a middle tank A; S2, adopting a feeding pump to deliver the filtrate to ceramic membrane separating equipment for concentration, adding water in a batched manner after being concentrated to 30-60% for dialysis until titer of concentrated liquid U is less than 500; S3, performing aftertreatment on the concentrated liquid, and enabling penetrating liquid treated by the ceramic membrane equipment to enter a middle tank B and then enter roll-type ultrafiltration membrane separating equipment to remove protein and part of pigment; S4, enabling the penetrating liquid to enter a middle tank C, using a nanofiltration membrane to concentrate the penetrating liquid, discharging or reusing the penetrating liquid, and finally enabling the concentrated liquid to enter a subsequent process to obtain a product. By using the process, quality of the concentrated liquid is improved, unit consumption of organic solvent is lowered by 70%, production cost is lowered substantially, and product quality is improved.

Description

technical field [0001] The invention relates to the technical field of medical application, in particular to a crystallization process of gibberellin fermentation liquid film extraction water. Background technique [0002] Gibberellin (English name: gibberellin), a widespread plant hormone. The chemical structure belongs to diterpenoid acids, derived from a tetracyclic skeleton. The current extraction (purification) process of gibberellin fermentation broth mainly adopts solvent method and macroporous resin method. [0003] Gibberellins are insoluble in water but soluble in organic solvents. Gibberellins can be extracted with methanol and purified with ethanol, but the extraction process with organic solvents has the following problems and disadvantages: [0004] 1. Completely using organic solvents to extract gibberellins will inevitably produce a large amount of solvent residues, which will affect the purity and yield of the final product. Especially, the average total ...

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Problems solved by technology

[0006] 3. The solvent extraction process of gibberellin concentrate needs to be heated, which not only increases the production cost, but also causes thermal damage to gibberellin, which affects product quality, and the product potency is lower than 8000γ / mL

[0008] 1. Impurities (proteins, sugars, etc.) contained in the feed liquid after film concentration and the eluent treated with macroporous resin cannot be removed, and the color is turbid, and solvent purification is still required in the later stage;

[0009] 2. The regeneration of macroporous resin requires the use of acid-base solvents, which will be emulsified during the extraction process;

[0010] 3. The resin regeneration cycle is long, and many sets of devices are switched to use, occupying a large area;

[0011] 4. The macroporous resin and film concentration process consume a lot of water, electricity and gas, and the operating cost is high

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