PCR (polymerase chain reaction) primer group, probe set and kit for detecting multiple respiratory pathogens

A technology for pathogens and respiratory tract, which is applied in recombinant DNA technology, microbial determination/inspection, resistance to vector-borne diseases, etc. It can solve the problem of not being able to detect respiratory pathogens, and achieve the effect of low cost, accurate detection, and avoidance of product contamination.

Active Publication Date: 2016-06-08
新疆亿立方生物技术有限公司
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Problems solved by technology

[0004] With the development of the medical level, the above-mentioned traditional methods can no longer satisfy the detection of respiratory pathogens. Clinically, there is a need for a sensitive, specific, fast and simple method for the identif

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  • PCR (polymerase chain reaction) primer group, probe set and kit for detecting multiple respiratory pathogens
  • PCR (polymerase chain reaction) primer group, probe set and kit for detecting multiple respiratory pathogens
  • PCR (polymerase chain reaction) primer group, probe set and kit for detecting multiple respiratory pathogens

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Embodiment Construction

[0031] The invention relates to a PCR primer set, a probe set, a PCR reaction solution and a kit for typing and detecting various respiratory pathogens. The present invention will be described in further detail below in combination with specific implementation methods and accompanying drawings. It should be emphasized that the following description is only exemplary and not intended to limit the scope of the invention and its application.

[0032] According to the embodiments of the present invention, the PCR primer set, probe set, PCR reaction solution and kit for detecting multiple respiratory pathogens will be used for typing detection of at least two of the following six respiratory pathogens, namely: adenocarcinoma Viruses, Bocavirus, Legionella pneumophila, Bordetella pertussis, Chlamydia pneumoniae, and Mycoplasma pneumoniae.

[0033] In some embodiments, the conserved gene fragments of respiratory pathogens from the nasopharyngeal swab of the subject can be amplified ...

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Abstract

The invention discloses a PCR (polymerase chain reaction) primer group, a probe set and a kit for detecting multiple respiratory pathogens. Sequences of PCR primers are represented as SEQ ID NO.1-SEQ ID NO.12; probes comprise at least two of six molecular beacon probes for respiratory pathogens, a fluorescence group and a quenching group are formed at two ends of each molecular beacon probe respectively, and sequences are represented as SEQ ID NO.13-SEQ ID NO.18. The invention further discloses a PCR reaction liquid containing the PCR primers and the probes as well as a kit containing the PCR reaction liquid for at least one person. With the adoption of the PCR primer group, the probe set and the kit, the six respiratory pathogens can be rapidly and accurately detected in a typing manner.

Description

technical field [0001] The invention relates to a PCR primer set, a probe set and a kit for detecting various respiratory pathogens. Background technique [0002] Respiratory tract infection is one of the most common clinical diseases, which can be caused by a variety of pathogens, including bacteria, viruses, mycoplasma, chlamydia, etc. Among them, acute respiratory tract infection (Acute Respiratory Tract Infection, ARTI) is an important cause of illness and death in children worldwide. The World Health Organization (WHO) published an article in the "Lancet" magazine in 2005 and reported that in 2000-2003, among the 10.6 million children under the age of 5 who died each year, 54% of them died from infectious diseases, of which 54% died from pneumonia. Children accounted for 19%. ARTI is strictly defined as all respiratory infections, however, in reality, the acute lower respiratory tract accounts for the majority of severe respiratory diseases, accounting for 20% of all ...

Claims

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Application Information

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IPC IPC(8): C12Q1/70C12Q1/68C12N15/11
CPCC12Q1/686C12Q1/689C12Q1/701C12Q2600/16C12Q2563/107C12Q2537/143Y02A50/30
Inventor 廖生赟许爱华冯传欣孙芳丁俊田梦
Owner 新疆亿立方生物技术有限公司
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