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Molecular marker for rice gel consistency control genes SBE1 and application of molecular marker

A molecular marker, glue consistency technology, applied in the field of agricultural biotechnology engineering, can solve problems such as poor accuracy, and achieve the effect of improving glue consistency

Active Publication Date: 2016-06-15
龙游翰儒农业科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Due to the effects of one cause and multiple effects, multiple causes and one effect, regulatory genes, and modified genes among genes, there are often large differences between individual phenotypes and genotypes, so the accuracy of individual selection through field phenotypic traits is poor

Method used

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  • Molecular marker for rice gel consistency control genes SBE1 and application of molecular marker
  • Molecular marker for rice gel consistency control genes SBE1 and application of molecular marker
  • Molecular marker for rice gel consistency control genes SBE1 and application of molecular marker

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0060] Example 1. Identify the polymorphisms of the indica rice special green with high glue consistency and the japonica rice Nipponbare with low glue consistency with Indel marker SBE1-m;

[0061] The specific method is as follows: select rice materials Nipponbare and Teqing, obtain their F1 by hybridizing Nipponbare and Teqing, and use primer SBE1-m to identify their polymorphisms ( figure 1 ).

[0062] 1. DNA extraction

[0063] 1), prepare DNA extraction buffer:

[0064] Add 1 volume of DNA extraction solution (0.35Msorbitol; 0.1MTris, pH8.2; 0.005MEDTA; the rest are water), 1 volume of nuclear lysis solution (0.2MTris, pH7.5; 0.05MEDTA; 2MNaCl; 0.055MCTAB) in order ; the rest are water) and 0.4 volume of 5% (mass concentration) sarkosyl solution (that is, an aqueous solution of sodium dodecanoyl-N-methylglycinate); finally, sodium bisulfite was added to prepare a DNA extraction buffer; bisulfite The final concentration of sodium in DNA extraction buffer was 0.02M.

...

Embodiment 2

[0084] Example 2. Using Indel molecular marker SBE1-m to identify the sequence differences between the indica rice Teqing with high glue consistency and the japonica rice Nipponbare with low glue consistency

[0085] The specific method is: using the Indel molecular marker SBE1-m to perform PCR amplification on the genomic DNA of Nipponbare and Teqing, and entrust Shanghai Yingjun Biotechnology Co., Ltd. to sequence the amplified products, and compare the differences in their sequences ( figure 2 ).

[0086] 1. DNA extraction

[0087] 1), prepare DNA extraction buffer:

[0088] Same as Example 1.

[0089] 2), the following treatments are respectively carried out to the rice leaves of the above-mentioned Nipponbare and Teqing:

[0090] Same as Example 1.

[0091] 3) PCR amplification

[0092] Same as Example 1.

[0093] 4) Recovery of PCR products

[0094] The recovery of PCR products was carried out using the PCR product recovery kit (spin column type, catalog number: ...

Embodiment 3

[0096] Example 3. Using Indel marker SBE1-m to carry out assisted selection breeding with high gel consistency

[0097] The specific method is as follows: the gene donor parent Teqing with high glue consistency and the japonica rice variety Nipponbare with low glue consistency are crossed, backcrossed and selfed in turn, and the obtained progeny are combined with the assisted selection of molecular marker SBE1-m, and the segregated population is selected. The single plant with the same band type as the ultra-green band type is used for breeding improvement.

[0098] 1. DNA extraction

[0099] 1), prepare DNA extraction buffer:

[0100] Same as Example 1.

[0101] 2), the rice leaves of above-mentioned Nipponbare, Teqing, gained progeny are respectively carried out as follows:

[0102] Same as Example 1.

[0103] 2. Indel marker detection

[0104] 1), PCR amplification

[0105] Same as Example 1.

[0106] 2), electrophoresis detection

[0107] Same as Example 1.

[010...

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Abstract

The invention discloses a molecular marker SBE1-m for rice gel consistency control genes SBE1. The molecular marker is characterized in that paddy rice is used as a species, primers of the molecular marker are selectively primer pairs, nucleotide sequences are 5'->3', the molecular marker SBE1-m in a forward direction is shown as CAAAGTGCTGACGTGGACAC, and the molecular marker SBE1-m in a reverse direction is shown as AAGAAAAGGAAGGAGAGACACG. The invention further provides application of the molecular marker SBE1-m. The molecular marker SBE1-m can be used for identifying different rice gel consistencies of the paddy rice and / or assisting in selectively breeding progenies of the paddy rice; single plants, with banding patterns consistent with Teqing banding patterns, of the progenies are selected for breeding when the progenies are Nipponbare and Teqing progenies.

Description

technical field [0001] The invention belongs to the field of agricultural biotechnology engineering, and particularly relates to a molecular marker related to the rice glue consistency control gene SBE1 and its application. Background technique [0002] High yield and high quality have always been the long-term goals of rice breeding. After long-term efforts, especially the utilization of hybrid rice technology, my country has achieved world-recognized achievements in rice production. However, since the problem of food and clothing has always been the first priority in the past, the focus of rice breeding work has mostly been on the cultivation of new high-yield varieties of rice, which has led to a serious delay in the breeding of high-quality rice, especially some high-yield hybrid rice. General quality deviation. [0003] Another main reason for the slow progress of rice quality improvement is the complexity of rice quality genetics and the limitations of traditional br...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N15/11C12Q1/68
CPCC12Q1/6895C12Q2600/13C12Q2600/156
Inventor 饶玉春徐江民蒋玲欢林晗沈晨辉黄苗苗符巧丽
Owner 龙游翰儒农业科技有限公司
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