Unlock instant, AI-driven research and patent intelligence for your innovation.

A kind of assay method of bacterial cell concentration in culture medium

A technology of bacteria concentration and measurement method, which is applied in the field of microbial fermentation, can solve problems such as large influence of medium composition, measurement interference, and measurement value interference, and achieve good practical value and popularization and application.

Active Publication Date: 2020-05-12
ZHUMADIAN HUAZHONG CHIA TAI
View PDF1 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, in actual use, this method is greatly affected by the composition of the medium
For example, if the fermentation medium contains insoluble components such as soybean powder, peanut powder, etc., it may contain a small amount of nucleic acid or nucleic acid analogues, and these substances are extracted into TCA together with the nucleic acid in the bacteria, thus bringing certain The degree of interference; especially when germ powder is used as a nutrient in the medium, it contains a high content of nucleic acid, which often causes serious interference to the measured value

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • A kind of assay method of bacterial cell concentration in culture medium
  • A kind of assay method of bacterial cell concentration in culture medium

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] The assay method of thalline concentration in the culture medium provided by the present embodiment specifically comprises the following steps:

[0030] (1) Take 5 mL of chlortetracycline fermentation broth fermented for 0 h (that is, before inoculation of the chlortetracycline fermentation medium in the fermentation tank), and keep the fermentation broth at 50°C for 30 minutes; after the incubation, the viscosity of the fermentation broth decreases, and the fluidity Better, there is no large amount of precipitation within 3 minutes.

[0031] What needs to be explained is that the viscosity of the fermentation broth with different fermentation times varies greatly. The viscosity is within 1s when not inoculated, while the viscosity of the fermentation broth during the normal fermentation process is about 1-15s. The viscosity of the fermentation broth is detected by a capillary viscometer .

[0032] Then add cellulase and pectinase, mix well to enzymolyze the fermentati...

Embodiment 2

[0044]The same as in Example 1, the concentration of nucleic acid in the same batch of aureomycin fermentation broth was measured, only the temperature of the fermentation broth in step (1) was adjusted to 45° C., and the incubation time was 30 minutes. The final calculation results showed that the mycelium nucleic acid concentration was 0.29mg / mL.

Embodiment 3

[0046] Same as Example 1, the nucleic acid concentration in the same batch of chlortetracycline fermentation broth is measured, only the ratio of biological enzymes during enzymolysis is adjusted, and the specific consumption of biological enzymes is: cellulase 0.07g, pectinase 0.13g , and the final calculated nucleic acid concentration was 0.06 mg / mL. From this result, it can be seen that the original amount of nucleic acid in the fermentation broth has almost no influence on the subsequent determination.

[0047] According to this operation method, the bacterium concentration in the fermentation liquid of the aureomycin fermentation liquid after different fermentation times is measured, and is measured according to the existing (i.e. the operation method in the comparative example) operation method at the same time, and the relevant measurement results are as follows: figure 1 shown. from figure 1 It can be seen that the improved TCA assay method provided by the present in...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention belongs to the technical field of microbial fermentation, and particularly relates to an improved method for measuring the concentration of thalli in compound culture media. The method includes steps of preserving heat for fermentation liquor; carrying out enzymatic hydrolysis; carrying out TCA (cellulose triacetate) extraction; measuring light absorbance; computing the concentration and the like. The improved method has the main advantages that the fermentation liquor is subjected to appropriate enzymatic hydrolysis treatment, accordingly, disturbance due to nucleic acid or analogs in materials such as soybean powder and peanut powder in solid components when the nucleic acid of the thalli is extracted from the culture media can be effectively prevented, the nucleic acid of the thalli in the fermentation liquor can be accurately and sufficiently extracted, the quantities of the thalli in the fermentation liquor can be truly reflected, reference and guidance can be effectively provided for fermentation production, and the improved method has excellent practical value and an excellent popularization and application significance.

Description

technical field [0001] The invention belongs to the technical field of microbial fermentation, and in particular relates to an improved method for measuring the concentration of bacteria in a compound culture medium. Background technique [0002] The determination of bacterial cell concentration is an important indicator to reflect the growth and metabolism of microorganisms, and more accurate data is needed to better control various production processes. In the existing industrial microbial fermentation process, since the fermentation medium containing a large amount of solid components is often used, such as soybean cake powder, peanut cake powder, etc., and these solid components are mixed with the bacteria, the determination of the concentration of bacteria in the fermentation liquid is difficult. become a complex and difficult issue that has remained unresolved for a long time. [0003] At present, most antibiotic factories generally use the packing volume method (cell...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/68C12Q1/06
CPCC12Q1/06
Inventor 李大攀李书至常坤郭双李俊陈浩然李冰
Owner ZHUMADIAN HUAZHONG CHIA TAI
Features
  • R&D
  • Intellectual Property
  • Life Sciences
  • Materials
  • Tech Scout
Why Patsnap Eureka
  • Unparalleled Data Quality
  • Higher Quality Content
  • 60% Fewer Hallucinations
Social media
Patsnap Eureka Blog
Learn More

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2025 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com