Reflection-type confocal system having high axial resolution

Abstract

The invention discloses a reflection-type confocal system having high axial resolution. The confocal system having high axial resolution comprises an illumination optical path and an imaging optical path, a laser and an imaging optical system used for focusing light emitted by a light source to an objected to be measured are arranged on the illumination optical path, an image detector capable of receiving the light returned from the objected to be measured and passing through the same imaging optical system is arranged on the imaging optical path; a semi-reflecting semi-transparent mirror, a galvanometer galvanometer group, a half-wave plate and a quarter-wave plate are arranged on the imaging optical system in order along a laser path, wherein the half-wave plate only shields a half optical path of the laser; wherein, a first polaroid is arranged between the laser and the imaging optical system, and a second polaroid having same polarization direction with the first polaroid is arranged on the imaging optical system and the image detector. The confocal system can remove the influence of stray light on imaging quality, so that the axial resolution and sectioning capability of the confocal imaging can be obviously increased.

Description

technical field [0001] The invention relates to confocal microscopes in the field of micro-optics. More specifically, the present invention relates to a reflective confocal system with high axial resolution. Background technique [0002] The reflective confocal system is one of the most advanced cell biomedical analysis instruments in modern times. It can perform optical tomography on the axial direction of the sample, so as to obtain a living image at the cell level at a depth of 200-350 μm, and reconstruct a three-dimensional image of the sample. The imaging of this system is closest to histopathology. It can not only observe fixed cells and tissue slices, but also observe and detect the structure, molecules and ions of living cells dynamically in real time. Among them, the axial resolution of the system is the most important index, which is directly related to the measurement accuracy of the system. [0003] The application research of confocal microscope in clinical de...

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Problems solved by technology

[0004] However, in the above improvements to the system, method (1) needs to detect polarized light separately, the imaging optical path is too complicated, and has high requirements on the detector; methods (2) and (3) need to use dual-frequency lasers, and the cost is high , also involves a feedback system, and the technology is complicated; method (4) has high requirements on the manufacturing precision and motion precision of the mechanical slit, and only half of the laser light enters the pupil of the objective lens, so it is difficult to realize the confocal scanning technology

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