A kind of preparation method of monoamine oxidase inhibitor

A monoamine oxidase and inhibitor technology, applied in the field of preparation of monoamine oxidase inhibitors, can solve the problems of few types, obvious toxic and side effects of chemical synthesis preparations, etc., and achieves the effects of good safety, easy popularization and application, and strong inhibitory effect.

Active Publication Date: 2020-08-14
BRIGHT DAIRY & FOOD CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] The technical problem to be solved by the present invention is to provide a method for preparing monoamine oxidase inhibitors in view of the fact that there are few types of monoamine oxidase inhibitors and the problem of obvious toxic and side effects of chemically synthesized preparations

Method used

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  • A kind of preparation method of monoamine oxidase inhibitor
  • A kind of preparation method of monoamine oxidase inhibitor
  • A kind of preparation method of monoamine oxidase inhibitor

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0056] Example 1 Separation and purification of monoamine oxidase inhibitors from the MRS fermentation broth of Lactobacillus casei BDII

[0057] (1) prepare the fermented liquid of Lactobacillus casei BD-II:

[0058] Lactobacillus casei BD-II (CGMCC No.0849) frozen at -80°C was inoculated on the MRS plate, and cultured anaerobically at 37°C for 48 hours; a single colony was picked from the plate and inoculated in 10 mL of MRS liquid medium. The primary seed liquid of BD-II was obtained by anaerobic culture at 37°C for 12 hours; then, the primary seed liquid was inoculated into 200 mL MRS liquid medium with a 4% inoculum size for expansion culture, and the similar step-by-step expansion culture was carried out to 6L. In a BIOTRON LiFlus SP_200L automatic fermenter at (35.5±0.5)°C, inoculate 6L of seed solution in 120L of MRS medium and ferment for 24 hours according to the inoculum size of 5%, to obtain 126L of fermentation stock solution (pH up to 4.00, the number of colonies...

Embodiment 2

[0080] Example 2 Separation and purification of monoamine oxidase inhibitors from the skim milk fermentation broth of Lactobacillus casei ATCC334

[0081] (1) prepare the fermented liquid of Lactobacillus casei ATCC334:

[0082] Lactobacillus casei ATCC334 frozen at -80°C was inoculated on the MRS plate and cultured anaerobically at 37°C for 48 hours; a single colony was picked from the plate and inoculated in 5 mL of skim milk and cultured anaerobically at 37°C for 12 hours to obtain a strain of ATCC334 Grade seed liquid; then this grade seed liquid is inoculated in 200mL skimmed milk with 4% inoculum size and carries out expanded culture, similar step by step expands culture to 6L, in BIOTRONLiFlus SP_200L automatic fermenter in (30.0 ± 0.5) Under the condition of ℃, 6L seed solution was inoculated in 120L skim milk and fermented for 48h according to the inoculum size of 5%, to obtain 126L fermentation stock solution (pH reached 4.53, and the number of colonies reached 10 9...

Embodiment 3

[0097] Example 3 Separation and purification of monoamine oxidase inhibitors from the skim milk fermentation broth of Lactobacillus bulgaricus LB340

[0098] (1) prepare the fermented liquid of Lactobacillus bulgaricus LB340:

[0099] Lactobacillus bulgaricus LB340 [DuPont Danisco (China) Co., Ltd.] frozen at -80°C was inoculated on the MRS plate and cultured anaerobically at 37°C for 48 hours; a single colony was picked from the plate and inoculated in 10 mL of skim milk in The primary seed liquid of LB340 was obtained by anaerobic culture at 37°C for 12 hours; then, the primary seed liquid was inoculated into 200 mL of skim milk with an inoculation volume of 4% for expanded culture, and similar step-by-step expansion culture was carried out to 6 L. In BIOTRON LiFlus SP_200L In a fully automatic fermenter at (40.0±0.5)°C, inoculate 6L of seed liquid into 120L of skim milk and ferment for 24 hours according to the inoculum size of 5%, to obtain 126L of fermentation stock solut...

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Abstract

The invention discloses a preparation method of a monoamine oxidase inhibitor. The preparation method comprises following steps: (1) ethyl alcohol is used for precipitating a lactobacillus fermentation broth, and a supernatant is collected; (2) water and ethyl acetate are added into the supernatant for extraction, an obtained ethyl acetate phase extracting solution is subjected to evaporation so as to remove the solvent and obtain an ester phase extract; and (3) the ester phase extract obtained in step (2) is subjected to column chromatography positive phase elution. The monoamine oxidase inhibitor is prepared from natural products, is obtained via separation purification from lactobacillus fermentation products, is low in toxicity, high in safety, and possesses a promising application prospect.

Description

technical field [0001] The invention relates to the fields of food, medicine and health products, in particular to a preparation method of a monoamine oxidase inhibitor. Background technique [0002] Population aging is a major problem facing mankind in the 21st century. Aging-related diseases, such as Alzheimer's disease (senile dementia), Parkinson's syndrome, etc., have gradually become the enemy of human health and a heavy burden on social prosperity, and effective drug treatment is essential to alleviate the disease . The root causes of such diseases are mostly related to the abnormal increase of monoamine oxidase activity in the human body. Therefore, finding or synthesizing substances with targeted inhibition of monoamine oxidase activity has become one of the important directions for the development of drugs for such diseases. [0003] Monoamine oxidase (monoamine oxidase, MAO, EC 1.4.3.4), the full name is monoamine oxidoreductase, is a kind of flavoproteinase, ma...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07C69/68C07C67/48C12P7/62A61P25/00A61P25/28A61P25/24C12R1/245C12R1/225C12R1/23
Inventor 游春苹吴正钧郭本恒刘振民韩瑨黄奇望
Owner BRIGHT DAIRY & FOOD CO LTD
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