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New nano-gold compound and preparation method thereof

A nano-gold, composite technology, applied in the direction of nanotechnology, nanotechnology for materials and surface science, nanotechnology, etc., can solve the problems of lack of versatility, waste of raw materials, limited application, etc.

Inactive Publication Date: 2016-06-22
SHANGHAI INST OF APPLIED PHYSICS - CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the yield of this method is low, time-consuming and labor-intensive, resulting in a great waste of raw materials, which greatly limits the further application of this method.
Some people have also improved the efficiency of single-binding nano-gold complexes to 70% by using steric hindrance, but this method lacks versatility and requires complicated and cumbersome operations.

Method used

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  • New nano-gold compound and preparation method thereof
  • New nano-gold compound and preparation method thereof
  • New nano-gold compound and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0107] Example 1 compares the differences in the assembly of BSPP-protected gold nanoparticles and block DNA under different pH conditions.

[0108] Experimental materials used: 10nm gold nanoparticles protected by BSPP, block DNA:

[0109] DNA: FAM-PolyA: AAAAAAA-FAM

[0110] Experimental principle: FAM-polyA is a piece of DNA composed of seven adenine nucleotides bound with a FAM fluorescent group at the 3' end. In the solution state, there is a strong fluorescence emission. When DNA is adsorbed on the surface of gold nanoparticles, the fluorescence is quenched, thereby reducing the fluorescence intensity in the solution. The amount of DNA adsorbed on the surface of gold nanoparticles was qualitatively analyzed by how much the fluorescence intensity decreased.

[0111] Experimental steps:

[0112] FAM-labeled polyADNA (FAM-polyA) and BSPP-protected gold nanoparticles were mixed, then citrate buffer solution with pH 3 or 7 was added, and the fluorescence value of the syste...

Embodiment 2

[0114] Embodiment 2 compares the assembly situation of the nanogold protected by BSPP to block DNA in different pH ranges

[0115] Experimental materials used: 10nm gold nanoparticles protected by BSPP:

[0116] polyA80-DNA:

[0117] AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATACGACGTGTGACCATGCA (SEQ ID NO.: 10)

[0118] Experimental procedure: Mix block DNA (polyA80-DNA) and BSPP-protected gold nanoparticles (100nM) at a ratio of 5:1, add trisodium citrate buffer (2.0; 3.0; 4.0; 5.0; 6.0; 7.0 ; 8.0), after standing for 10 minutes, carry out agarose gel electrophoresis characterization.

[0119] The above experimental results show that (see Figure 4 ), the block DNA can be assembled and adsorbed on the BSPP-protected gold nanoparticles within 10 minutes only under acidic conditions (pH2-4).

Embodiment 3

[0120] The assembly situation of polyA80-DNA in the scope of embodiment 3pH2.0-4.0

[0121] Experimental procedure: Mix block DNA (polyA80-DNA) and BSPP-protected gold nanoparticles (100nM) at a ratio of 5:1, add trisodium citrate buffer (2.0; 2.4; 2.8; 2.9; 3.0; 3.1 ; 3.2; 3.3; 3.4; 3.5; 3.6; 4.0), after standing for 10 minutes, agarose gel electrophoresis was performed.

[0122] The above experimental results show that (see Figure 5 ), in the range of pH 2-3.6, the higher the pH value, the fewer the number of block nucleic acids bound on the gold nanoparticles. In the case of pH 3.1, polyA80-DNA can achieve 90% single binding yield. The amount of binding assembly gradually increases with the decrease of pH, and quantitative binding such as double binding or triple binding can be achieved when the pH is lower than 3.1.

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Abstract

The invention discloses a new block nucleic acid, a nano-gold compound combined with the new block nucleic acid, and a preparation method of the nano-gold compound. The pH value and the length of adenine in the block nucleic acid are adjusted, so the assembling combination efficiency of the nano-gold compound is effectively improved, and the combination quantity of the block nucleic acid in the nano-gold compound is quantitatively controlled. The method has the advantages of high efficiency, fast assembling speed, uniform and controllable product, and realization of entrance of the product to downstream without purifying.

Description

technical field [0001] The invention belongs to the technical field of novel material synthesis, and relates to a novel, fast and high-yield method for preparing quantitative DNA-bound nano gold. Background technique [0002] Nanogold is a commonly used nanomaterial. In order to better exert its function, some functional molecules are often combined specifically on the surface of nanogold to form a nanogold composite. Nanogold composites are widely used in bioanalysis, bioimaging and biopharmaceuticals, as well as self-assembly. Although some achievements have been made, there is a problem that cannot be ignored, that is, in these applications, the number of functional molecules such as DNA bound to each nanogold is not fixed, and this inaccuracy plays an important role in promoting this paper. Further development of the field brings with it a great deal of uncertainty. [0003] To overcome this uncertainty, quantitative DNA-bound gold nanoparticle complexes can be prepare...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N15/11B22F1/00B82Y30/00B82Y40/00
Inventor 樊春海姚广保裴昊李江
Owner SHANGHAI INST OF APPLIED PHYSICS - CHINESE ACAD OF SCI