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Gene for regulating plant types and yield of plants and application of gene

A yield and plant technology, applied in the fields of plant genetic improvement, plant products, and botanical equipment and methods, which can solve problems such as narrow gene pools

Active Publication Date: 2016-06-22
CAS CENT FOR EXCELLENCE IN MOLECULAR PLANT SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] As an important crop, rice has undergone the domestication process from wild rice to cultivated rice. This process has produced many germplasm resources, which contain a large number of excellent natural variations. Although the current rice breeding technology and process have been perfected, the current The bottleneck has been encountered, and the existing gene pool tends to be narrow. In order to further increase production, it is urgent to find new natural variations to improve existing varieties. Finding suitable germplasm is an important prerequisite for realizing this wish

Method used

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  • Gene for regulating plant types and yield of plants and application of gene
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  • Gene for regulating plant types and yield of plants and application of gene

Examples

Experimental program
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Effect test

Embodiment 1

[0189] Embodiment 1, new plant type germplasm excavation and main effect gene location

[0190] According to the definition of the new plant type by the International Rice Institute, the inventor has discovered a new type of rice germplasm in the germplasm resources of the Zhejiang area, and named it WS3 ( figure 1 A left). Compared with the control variety Nipponbare (Nip figure 1 A right) Compared with the WS3 stem diameter is about 0.7-0.8cm ( figure 1 C-F), the average number of tillers is 5, and the number of primary branches is as high as 30 ( figure 1 C), the number of spikelets per ear exceeds 300 ( figure 1 B), with typical new plant type characteristics. Therefore, this germplasm was used as the raw material for subsequent gene mining, and was crossed with Nipponbare to construct F2 population for QTL analysis.

[0191] By using 145 polymorphic molecular markers evenly distributed on the 12 chromosomes of rice, the inventor constructed a saturated genetic linkag...

Embodiment 2

[0192] Example 2. Identification of the unique structure of qWS8 and its application

[0193] By designing primers across the localization interval, the inventors amplified the sequence corresponding to WS3 using the genomes of Nipponbare (Nip) and WS3 as amplification templates, but did not find any difference in sequence size. When detecting by hybridization technology, it can be detected that the sequence size of the positioning interval is different, and the WS3 interval is obviously larger than the Nip interval. When XbaI single enzyme digestion and double digestion with other enzymes, combined with the probe of the sequence in the positioning interval, it can be detected These results indicate that WS3 contains a repeat sequence that cannot be detected by conventional PCR, and these repeat sequences are completely consistent ( Figure 5 ).

[0194] By using a special high-fidelity Taq enzyme, the inventors successfully amplified the full-length WS3 region, and subcloned...

Embodiment 3

[0197] Example 3, qWS8 loci affect multiple important agronomic traits

[0198] Through detailed phenotypic analysis of the four key exchanges (R7, R8, R9, R10-B, R10 was backcrossed to form the segregant line R10-B), it was found that qWS8 not only had a significant promoting effect on stem diameter , and can greatly increase the number of first-level branches, while moderately reducing the number of tillers ( Figure 8 , different letters indicate significant differences), these all meet the new plant type standards defined by the international rice, so the inventors believe that qWS8 is an important site for generating new rice plant types.

[0199] In addition, this locus has no obvious promotion effect on plant height or the effect is not large, suggesting that the promotion of this locus on plant height may depend on specific downstream genes, but overall, the negative effect of qWS8 on plant height is not large. In order to further illustrate the contribution of qWS8 t...

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Abstract

The invention relates to a gene for regulating the plant types and the yield of plants and application of the gene. First-time separation is carried out to obtain polynucleotide for regulating the plant type and the yield trait relevance of the gramineous plants, and polynucleotide can promote the improvement of the plant type of the gramineous plants remarkably, promote increase of the diameter of stems, promote increase of the ear first-level branch number, the ear second-level branch number, the spikelet number of each ear, the weight of each ear or the length of each ear, reduce the tiller number properly, promote increase of the plant height and promote increase of the yield of the gramineous plants. The invention further provides a molecular marker used for identifying fine traits of the plants. A new path for improving the traits of the gramineous plants is provided.

Description

technical field [0001] The invention relates to the fields of botany and gene technology, more specifically, the invention relates to genes for regulating plant type and yield and applications thereof. Background technique [0002] Grasses, especially rice, as an important food crop, feed about 1 / 2 of the world's population. However, as the population continues to increase and the available arable land decreases, further increasing plant yield per unit area has become the main goal of current breeding. Excavating new germplasm resources and their dominant genes has become an effective way to achieve this goal. In the 1950s and 1960s, the discovery of the dwarf variety Banana Nantes rice brought the first green revolution to world food production. The GA20 oxidase mutation of this variety produced a semi-dwarf phenotype, which increased the lodging resistance of rice plants and fertilizer tolerance, and ultimately increased harvest index. With the development of dwarf breed...

Claims

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Application Information

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IPC IPC(8): C12N15/29C12N15/82C12Q1/68C12N15/11A01H1/02A01H1/04A01H5/00
Inventor 何祖华张林李群
Owner CAS CENT FOR EXCELLENCE IN MOLECULAR PLANT SCI
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