A kind of breeding method of upland rice two-line sterile line
A sterile line and feeding technology, which is applied in the field of breeding two-line male sterile line of upland rice for feeding, can solve the problems of increased difficulty in breeding work, late research work on feed rice, and insufficient breeding research.
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Embodiment 1
[0034] (1) Parent selection: the forage rice variety "Jinzao 47" with high yield, brown rice crude protein content greater than 12%, and brown rice yield greater than 80% was selected as the basic parent (P), and the grain-stalk dual-purpose rice variety "Dongnan 202" ( P1), the high-yield and stress-resistant upland rice variety "Huhan 7" (P2) was used as the general parent, and the two-line maintainer "Peiai 64S" (R) was used as the recurrent parent;
[0035] (2) Planting and selection of hybrid offspring:
[0036] a. Plant the parents P, P1, P2 and R respectively, and make crosses P×P1→F at the flowering stage 1-1 , P×P2→F 1-2 , R×P→F 1-3 ; Separately plant hybrid F 1 Generation F 1-1 , F 1-2 , F 1-3 Cross P×F with parent P at flowering stage 1-1 , P×F 1-2 and F 1-3 ×P, get backcross F 1 Generation BF 1-1 , BF 1-2 and BF 1-3 ;
[0037] b. Plant BF separately 1-1 , BF 1-2 and BF 1-3 , as hybrid BF at flowering stage 1-1 ×BF 1-2 →BF 1-12 , BF 1-3 Harvest ...
Embodiment 2
[0050] (1) Parental selection: the forage rice variety "Xiangzaoxian 24" with high yield, brown rice crude protein content greater than 12%, and brown rice yield greater than 80% was selected as the basic parent (P), and the grain-stalk dual-purpose rice variety "Dongnan 202" was selected as the basic parent (P). "(P1), the high-yielding and stress-resistant upland rice variety "Linhan 1" (P2) was used as the general parent, and the two-line maintainer "Fu eS1" (R) was used as the recurrent parent;
[0051] (2) Planting and selection of hybrid offspring:
[0052] a. Plant the parents P, P1, P2 and R respectively, and make crosses P×P1→F at the flowering stage 1-1 , P×P2→F 1-2 , R×P→F 1-3 ; Separately plant hybrid F 1 Generation F 1-1 , F 1-2 , F 1-3 Cross P×F with parent P at flowering stage 1-1 , P×F 1-2 and F 1-3 ×P, get backcross F 1 Generation BF 1-1 , BF 1-2 and BF 1-3 ;
[0053] b. Plant BF separately 1-1 , BF 1-2 and BF 1-3 , as hybrid BF at flowering s...
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