Magnetic-targeted nanodiamond drug and preparation method and application thereof

A nanodiamond, magnetic targeting technology, applied in drug combinations, pharmaceutical formulations, antitumor drugs, etc., can solve problems such as impact, poor biocompatibility and low toxicity, and achieve the effect of easy detection

Inactive Publication Date: 2016-06-29
SHANXI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, these nanomaterials have poor biocompatibility and low toxicity, which seriously affect their use as drug carriers.

Method used

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  • Magnetic-targeted nanodiamond drug and preparation method and application thereof
  • Magnetic-targeted nanodiamond drug and preparation method and application thereof
  • Magnetic-targeted nanodiamond drug and preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] (1) Magnetic Fe 3 O 4 Preparation of nanoparticles

[0033] Accurately weigh 0.1987g FeCl 2 .4H 2 O and 0.5406g FeCl 3 .6H 2O, dissolve in 100ml deionized water, add 1.0ml 0.1M sodium citrate solution and mix well, adjust the solution with 0.1M NaOH under vigorous stirring to make the pH=11, and continue stirring for 30min, then put the mixed solution at 80 °C oil The reaction was carried out in the bath for 2 h to obtain a precipitate. After suction filtration, the solution was washed with deionized water for 5 times, and then dried in vacuum for use.

[0034] (2) Magnetic Fe 3 O 4 - Preparation of PLL nanoparticles

[0035] Accurately weigh the magnetic 3mgFe 3 O 4 Dissolve in 3ml MES (0.1M) pH5.8 buffer solution, ultrasonically disperse; add 10mg NHS, 9mgEDC into the above solution, ultrasonically disperse for 30min, add 420ul (1μg / μl) PLL, shake well for 6h, wash with deionized water for 3 times, vacuum drying for use.

[0036] (3) Magnetic Fe 3 O 4 - ...

Embodiment 2

[0041] (1) Magnetic Fe 3 O 4 Preparation of nanoparticles

[0042] Accurately weigh 0.1987g FeCl 2 .4H 2 O and 0.5406g FeCl 3 .6H 2 O, dissolve in 100ml deionized water, add 5.0ml 0.1M sodium citrate solution and mix well, adjust the solution with 0.1M NaOH under vigorous stirring to make the pH=11, and continue stirring for 30min, then the mixed solution is heated at 80℃ The reaction was carried out in the bath for 2 h to obtain a precipitate. After suction filtration, the solution was washed with deionized water for 5 times, and then dried in vacuum for use.

[0043] The steps of (2)-(4) are the same as the implementation case 1.

Embodiment 3

[0045] (1) Magnetic Fe 3 O 4 Preparation of nanoparticles

[0046] Accurately weigh 0.1987g FeCl 2 .4H 2 O and 0.5406g FeCl 3 .6H 2 O, dissolve in 100ml deionized water, add 10.0ml 0.1M sodium citrate solution and mix well, adjust the solution with 0.1M NaOH under vigorous stirring to make the pH=11, and continue stirring for 30min, then the mixed solution is heated at 80℃ The reaction was carried out in the bath for 2 h to obtain a precipitate. After suction filtration, the solution was washed with deionized water for 5 times, and then dried in vacuum for use.

[0047] The steps of (2)-(4) are the same as the implementation case 1.

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Abstract

The invention provides a magnetic-targeted nanodiamond drug and a preparation method and application thereof. The method comprises the steps that a magnetic nanodiamond composite material (Fe3O4-PLL-ND) is formed by magnetic Fe3O4 subjected to carboxylation and nanodiamond (ND) under the action of a coupling agent polylysine (PLL); an anti-cancer drug doxorubicin (DOX) is physically adsorbed by the magnetic nanodiamond composite material, and the magnetic-targeted nanodiamond drug (Fe3O4-PLL-ND-DOX) is obtained. Under the action of an external magnetic field, the magnetic-targeted drug acts with human HeLa cells, and it is shown that the magnetic-targeted drug can inhibit tumor growth in a targeted mode; tumor-bearing mice serve as models, the magnetic-targeted effect of the drug in mouse bodies is researched, and it is shown that the drug can locate to tumor locations in a targeted mode. The magnetic-targeted nanodiamond drug can be applied in preparation of magnetic-targeted anti-tumor drugs.

Description

technical field [0001] The invention relates to a targeted nano-drug, in particular to a magnetic-targeted nano-diamond drug loaded with magnetic ferric tetroxide and a chemotherapeutic drug doxorubicin after the surface of the nano-diamond is modified, and a preparation method thereof, as well as the drug used in the preparation of an anti-tumor drug Applications. Background technique [0002] Today, cancer has become the second leading killer of human health after cardiovascular disease. The survival rate of malignant tumors is very low, and the main reason is that tumor patients are already in the middle or late stage when they find the disease, and they miss the best time for surgery, radiotherapy and chemotherapy. In addition, traditional chemotherapeutic drugs lack pharmacological unity and have great cytotoxic side effects on normal tissue, which is also the main reason for the low efficiency of cancer treatment. It can be seen that the effective way to improve the ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K47/34A61K47/04A61K41/00A61K31/704A61P35/00
CPCA61K31/704A61K41/0052A61K47/02A61K47/34
Inventor 李英奇赵文静王志琴
Owner SHANXI UNIV
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