A magnetically targeted nano-diamond drug and its preparation method and application

A nanodiamond, magnetic targeting technology, applied in drug combinations, pharmaceutical formulations, antitumor drugs, etc., can solve problems such as impact, poor biocompatibility and low toxicity, and achieve the effect of easy detection

Inactive Publication Date: 2019-01-15
SHANXI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, these nanomaterials have poor biocompatibility and low toxicity, which seriously affect their use as drug carriers.

Method used

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  • A magnetically targeted nano-diamond drug and its preparation method and application
  • A magnetically targeted nano-diamond drug and its preparation method and application
  • A magnetically targeted nano-diamond drug and its preparation method and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] (1) Magnetic Fe 3 o 4 Preparation of nanoparticles

[0033] Accurately weigh 0.1987gFeCl 2 .4H 2 O and 0.5406 g FeCl 3 .6H 2O, dissolve in 100ml deionized water, add 1.0ml 0.1M sodium citrate solution and mix well, adjust the solution with 0.1M NaOH under vigorous stirring to make the pH = 11, and keep stirring for 30min, then put the mixed solution at 80°C React in an oil bath for 2 hours to obtain a precipitate, which is filtered by suction, washed 5 times with deionized water, and dried in vacuum for later use.

[0034] (2) Magnetic Fe 3 o 4 - Preparation of PLL nanoparticles

[0035] Accurately weigh 3mg Fe magnetically 3 o 4 Dissolve in 3ml MES (0.1M) pH5.8 buffer solution, ultrasonically disperse; weigh 10mg NHS, add 9mg EDC to the above solution, ultrasonically disperse for 30min, add 420ul (1μg / μl) PLL, shake well for 6h, wash with deionized water Wash 3 times and dry in vacuum for later use.

[0036] (3) Magnetic Fe 3 o 4 - Preparation of PLL-ND n...

Embodiment 2

[0041] (1) Magnetic Fe 3 o 4 Preparation of nanoparticles

[0042] Accurately weigh 0.1987gFeCl 2 .4H 2 O and 0.5406 g FeCl 3 .6H 2 O, dissolve in 100ml deionized water, add 5.0ml 0.1M sodium citrate solution and mix well, adjust the solution with 0.1M NaOH under vigorous stirring to make the pH = 11, and keep stirring for 30min, then put the mixed solution at 80°C React in an oil bath for 2 hours to obtain a precipitate, which is filtered by suction, washed 5 times with deionized water, and dried in vacuum for later use.

[0043] The steps of (2)-(4) are the same as the implementation case 1.

Embodiment 3

[0045] (1) Magnetic Fe 3 o 4 Preparation of nanoparticles

[0046] Accurately weigh 0.1987gFeCl 2 .4H 2 O and 0.5406 g FeCl 3 .6H 2 O, dissolve in 100ml of deionized water, add 10.0ml of 0.1M sodium citrate solution and mix well, adjust the solution with 0.1M NaOH under vigorous stirring to make the pH = 11, and keep stirring for 30min, then put the mixed solution at 80°C React in an oil bath for 2 hours to obtain a precipitate, which is filtered by suction, washed 5 times with deionized water, and dried in vacuum for later use.

[0047] The steps of (2)-(4) are the same as the implementation case 1.

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Abstract

The invention provides a magnetic-targeted nanodiamond drug and a preparation method and application thereof. The method comprises the steps that a magnetic nanodiamond composite material (Fe3O4-PLL-ND) is formed by magnetic Fe3O4 subjected to carboxylation and nanodiamond (ND) under the action of a coupling agent polylysine (PLL); an anti-cancer drug doxorubicin (DOX) is physically adsorbed by the magnetic nanodiamond composite material, and the magnetic-targeted nanodiamond drug (Fe3O4-PLL-ND-DOX) is obtained. Under the action of an external magnetic field, the magnetic-targeted drug acts with human HeLa cells, and it is shown that the magnetic-targeted drug can inhibit tumor growth in a targeted mode; tumor-bearing mice serve as models, the magnetic-targeted effect of the drug in mouse bodies is researched, and it is shown that the drug can locate to tumor locations in a targeted mode. The magnetic-targeted nanodiamond drug can be applied in preparation of magnetic-targeted anti-tumor drugs.

Description

technical field [0001] The invention relates to targeted nano-medicines, in particular to a magnetic-targeted nano-diamond drug loaded with magnetic ferric iron tetroxide and chemotherapeutic drug doxorubicin after the surface modification of nano-diamonds and a preparation method thereof, and the use of the drug in the preparation of anti-tumor drugs Applications. Background technique [0002] Today, cancer has become the second largest killer of human health, second only to cardiovascular disease. The survival rate of malignant tumors is very low. The main reason is that tumor patients are already in the middle or late stage when they are diagnosed, and they miss the best time for surgery, radiotherapy and chemotherapy. In addition, traditional chemotherapeutic drugs lack pharmacological unity and have severe side effects on normal tissue cells, which is also the main reason for the low efficiency of cancer treatment. It can be seen that the effective way to improve the ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K47/34A61K47/04A61K41/00A61K31/704A61P35/00
CPCA61K31/704A61K41/0052A61K47/02A61K47/34
Inventor 李英奇赵文静王志琴
Owner SHANXI UNIV
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