Application of chikusetsusaponin IVa to preparation of antitumor drugs

A technology of bamboo ginseng saponins and anti-tumor drugs, applied in the directions of anti-tumor drugs, drug combinations, pharmaceutical formulations, etc., can solve problems such as unreported, and achieve a good inhibitory effect.

Inactive Publication Date: 2016-07-20
JIAN SHENGLIYUAN BIOTECH DEV CO LTD
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Except in 2015 Lee, K.M. reported that its derivative bamboo ginsenoside IVa methyl ester has cytotoxic activity on HCT116 cells [Lee, K.M., et al., Chikusetsu saponin IVamethylester induces cell cycle arrest by the inhibition of nuclear translocation of beta-cateninin HCT116 cells. Biochem Biophys ResCommun, 2015.4599 (4) There are no reports on the application of bamboo ginsenoside IVa as the main component in the preparation of antitumor drugs

Method used

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  • Application of chikusetsusaponin IVa to preparation of antitumor drugs
  • Application of chikusetsusaponin IVa to preparation of antitumor drugs
  • Application of chikusetsusaponin IVa to preparation of antitumor drugs

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Experimental program
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Embodiment

[0040] 1. Test material

[0041] 1.1 Drugs and reagents

[0042] DMEM high-glucose medium was purchased from Gibco Company, FBS fetal bovine serum was purchased from Clark Company, and trypsin was purchased from Beijing Suolaibao Biotechnology Co., Ltd.

[0043] 1.2 Cells and animals

[0044] Highly metastatic mouse melanoma cells B16F10 were purchased from the Shanghai Cell Bank of the Chinese Academy of Sciences. C57BL / 6 mice, male, 20-22 g, were purchased from Beijing Weitong Lihua Experimental Animal Technology Co., Ltd.

[0045] 1.3 Animal model

[0046] 1.3.1 Cell recovery: take out the cells frozen in liquid nitrogen, quickly place them in a 37°C water bath, melt them into a liquid state, place them in a clean bench after disinfection, and transfer the cell liquid to a 10ml centrifuge tube after blowing evenly , add 2ml of DMEM high-glucose medium, 1000rpm, centrifuge for 5min, take it out and discard the supernatant, add DMEM high-glucose medium containing 10% FBS ...

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Abstract

The invention mainly relates to application of chikusetsusaponin IVa to preparation of antitumor drugs. Through studies on change experiments about the tumor size of a mouse injected with the chikusetsusaponin IVa, it is discovered that the rate that the chikusetsusaponin IVa inhibits growth of a tumor in the mouse in early period is basically unchanged, the tumor in the mouse injected with the chikusetsusaponin IVa increases in the same pace with that in animals of a model group, but after the tumor enters a logarithmic growth period, the chikusetsusaponin IVa has an increasingly inhibiting effect on enlarging of the tumor in the mouse and has the inhibition rate slightly higher than that in a slow growing period, and accordingly the chikusetsusaponin IVa has a good inhibiting effect on melanomas of the mouse. It is discovered that the chikusetsusaponin IVa has a good inhibiting effect on the melanomas of the mouse through studies on change experiments about tumor weight of the mouse injected with the chikusetsusaponin IVa and can be prepared into novel antitumor drugs.

Description

technical field [0001] The present invention relates to the application of bamboo ginsenoside IVa, in particular to the application of bamboo ginsenoside IVa in the preparation of anti-melanoma drugs. Background technique [0002] Malignant tumor, that is, cancer, is a common and frequently-occurring disease caused by the unlimited proliferation of endogenous cells, which seriously threatens human health, and is one of the three major diseases that cause human death. [0003] Currently, cancer treatment methods mainly include surgery, radiotherapy and chemotherapy. Chemotherapy is a treatment method that uses chemical drugs to block the mechanism of cell division to inhibit the growth and reproduction of cancer cells and promote the differentiation of cancer cells. It is also the main method for the treatment of malignant tumors. However, traditional chemotherapy drugs have a series of limitations in the treatment of cancer. For example, common chemotherapy drugs such as c...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K31/704A61P35/00A61P35/02
CPCA61K31/704
Inventor 张楠淇肖禹安
Owner JIAN SHENGLIYUAN BIOTECH DEV CO LTD
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