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Ultralow-temperature preservation method for haematococcus pluvialis subjected to aseptic treatment

A technology of Haematococcus pluvialis and Haematococcus pluvialis algae liquid, which is applied in the field of cryopreservation of Haematococcus pluvialis germplasm, achieving good recovery survival rate and not easy aging effect

Pending Publication Date: 2016-07-20
STATE DEV & INVESTMENT +1
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  • Application Information

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Problems solved by technology

For the cryopreservation of Haematococcus pluvialis, due to the influence of various factors, there is no relevant technical report

Method used

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Embodiment 1

[0026] In this embodiment, Haematococcus pluvialis FACHB-712 is taken as an example to illustrate the method of the present invention. The Haematococcus pluvialis used in this example was detected as bacteria-containing Haematococcus pluvialls by coating the LB plate.

[0027] A cryopreservation method for aseptically treated Haematococcus pluvialis, comprising:

[0028] 1). Treatment group (a total of three groups of repetitions): Add ampicillin with a final concentration of 300ug / ml to 30ml of Haematococcus pluvialis FACHB-712 algae liquid in the late stage of exponential growth, cultivate for 2 days, and continue to add a final concentration of 5ug / ml of gentamycin, cultivated for 2 days, continued to add kanamycin with a final concentration of 5ug / ml, and cultivated for another 2 days.

[0029] Control group: except that antibiotics were not added, other culture conditions were the same as those of the treatment group.

[0030] The above culture conditions: the light int...

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Abstract

The invention discloses an ultralow-temperature preservation method for haematococcus pluvialis subjected to aseptic treatment. The ultralow-temperature preservation method has the advantages that systematic sterilization work is carried out before the haematococcus pluvialis is preserved, the thawed aseptic haematococcus pluvialis is excellent in resuscitation survival rate owing to specific low-temperature preservation conditions, and the resuscitation survival rate of the haematococcus pluvialis can reach 66.13%; sterilized bacterial liquid on LB panels is free of bacterial colonies, and is free of bacterial colonies after the bacterial liquid is continuously cultivated thrice and is subjected to aseptic detection by the aid of the LB panels, sterilized haematococcus pluvialis strains are difficult to age, and accordingly effects of keeping the germplasm genetic stability and bringing convenience for storing the haematococcus pluvialis for a long time can be realized.

Description

technical field [0001] The invention relates to the technical field of microalgae cultivation with high added value, and more specifically to a method for cryopreservation of aseptically treated Haematococcus pluvialis germplasm Background technique [0002] Haematococcus pluvialis belongs to Chlorophyta (Chlorophyta) Chlorophyceae (Chlorophyceae) Volvocales (Volvocales) Haematococcaceae (Haematococcaceae) Haematococcus (haematococcus), when the environment is bad, its green swimming The cells transform into red quiescent cells and accumulate astaxanthin. Astaxanthin is a high-value biologically active product with strong antioxidant activity and is widely used in aquaculture, food, cosmetics, pharmaceuticals and other industries. As the content of astaxanthin in Haematococcus pluvialis is the highest, it is recognized as the best biological source of astaxanthin in nature, so it has become a hot spot in the development and application of algae in recent years. The sterili...

Claims

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Application Information

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IPC IPC(8): C12N1/04C12N1/12C12R1/89
CPCC12N1/04C12N1/12
Inventor 郑凌凌宋立荣张琪李天丽
Owner STATE DEV & INVESTMENT