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Medium for human umbilical blood mesenchymal stem cells and culture method thereof

A technology of mesenchymal stem cells and culture methods, which is applied in the medium of umbilical cord blood mesenchymal stem cells and its culture field, can solve the problems of low culture efficiency and limited application, and achieve the purpose of improving the ability of proliferation, maintaining phenotype and characteristics, and promoting The effect of cell proliferation

Active Publication Date: 2016-07-20
GUANGZHOU SALIAI STEMCELL SCI & TECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, due to the particularity of the source and storage of cord blood, its application is limited.
It has been reported to use adult autologous serum as a serum substitute to culture umbilical cord mesenchymal stem cells; however, the culture efficiency is lower than that of umbilical cord serum

Method used

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  • Medium for human umbilical blood mesenchymal stem cells and culture method thereof
  • Medium for human umbilical blood mesenchymal stem cells and culture method thereof
  • Medium for human umbilical blood mesenchymal stem cells and culture method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0047] (1) Experimental grouping

[0048] Table 1 Experimental Grouping Situation

[0049] group

Medium composition

control group

DMEM / F12+10%FBS

Experimental group 1

DMEM / F12+10%PRP

Experimental group 2

DMEM / F12+1mg / mL astragalus polysaccharide

Experimental group 3

DMEM / F12+10%PRP+1mg / mL astragalus polysaccharide

[0050] (2) Adult autologous serum preparation

[0051] The peripheral blood of normal volunteers was drawn into a sterile glass bottle and placed in an incubator for 2 hours. After the blood clot was precipitated, the serum was aspirated and transferred into a centrifuge tube, centrifuged at 1500 rpm for 20 minutes at low temperature, the precipitate was discarded, and PRP was obtained and stored in aliquots. Inactivate in a 60°C water bath for 20 minutes before use, and store in a 4°C refrigerator for later use. Prepare the medium as in the experimental grouping.

[0052] (3) Primary isolation of u...

Embodiment 2

[0075] (1) Experimental grouping

[0076] Table 4 Experimental Grouping Situation

[0077] group

Medium composition

control group

DMEM / F12+10%FBS

Experimental group 1

DMEM / F12+8%PRP

Experimental group 2

DMEM / F12+0.5mg / mL astragalus polysaccharide

Experimental group 3

DMEM / F12+8%PRP+0.5mg / mL astragalus polysaccharide

[0078] (2) Adult autologous serum preparation

[0079] The peripheral blood of normal volunteers was drawn into a sterile glass bottle and placed in an incubator for 2 hours. After the blood clot was precipitated, the serum was aspirated and transferred into a centrifuge tube, centrifuged at 1500 rpm for 20 minutes at low temperature, the precipitate was discarded, and PRP was obtained and stored in aliquots. Inactivate in a 60°C water bath for 20 minutes before use, and store in a 4°C refrigerator for later use. Prepare the medium as in the experimental grouping.

[0080] (3) Primary isolation of...

Embodiment 3

[0103] (1) Experimental grouping

[0104] Table 7 Experimental Grouping Situation

[0105] group

Medium composition

control group

DMEM / F12+10%FBS

Experimental group 1

DMEM / F12+5%PRP

Experimental group 2

DMEM / F12+0.1mg / mL astragalus polysaccharide

Experimental group 3

DMEM / F12+5%PRP+0.1mg / mL astragalus polysaccharide

[0106] (2) Adult autologous serum preparation

[0107] The peripheral blood of normal volunteers was drawn into a sterile glass bottle and placed in an incubator for 2 hours. After the blood clot was precipitated, the serum was aspirated and transferred into a centrifuge tube, centrifuged at 1500 rpm for 20 minutes at low temperature, the precipitate was discarded, and PRP was obtained and stored in aliquots. Inactivate in a 60°C water bath for 20 minutes before use, and store in a 4°C refrigerator for later use. Prepare the medium as in the experimental grouping.

[0108] (3) Primary isolation of...

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Abstract

The invention relates to the technical field of cell culture and particularly relates to a medium for human umbilical blood mesenchymal stem cells and a culture method thereof. The medium comprises PRP (platelet rich plasma), astragalus polysaccharide and a serum-free medium. The medium provided by the invention can remarkably increase the proliferation efficiency of the human umbilical blood mesenchymal stem cells and maintain the phenotype and features of the human umbilical blood mesenchymal stem cells while promoting cell proliferation, and can reduce the risk and immunogenicity of animal serum application to realize good safety.

Description

technical field [0001] The invention relates to the technical field of cell culture, in particular to a medium for umbilical cord blood mesenchymal stem cells and a culture method thereof. Background technique [0002] Mesenchymal stem cells (MSCs) are pluripotent adult stem cells that are ubiquitous in different tissues, mainly in connective tissue and organ interstitium, and are most abundant in bone marrow tissue. They can stimulate tissue growth and repair, and enhance tissue regenerative capacity. There are cell populations similar to bone marrow mesenchymal stem cells and adipose mesenchymal stem cells in umbilical cord blood, which have the ability to differentiate into various cells under specific induction conditions, such as osteogenic differentiation and adipogenic differentiation. Human umbilical cord blood mesenchymal stem cells have been used in animal models of diabetes, neurological diseases, and heart disease. Due to the advantages of simple collection, hi...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N5/0775
CPCC12N5/0665C12N2500/84C12N2500/90C12N2501/90
Inventor 葛啸虎陈海佳王一飞马岩岩
Owner GUANGZHOU SALIAI STEMCELL SCI & TECH CO LTD