Method for promoting diffusion of altar laver shell protonema conchospore

A technology of conch filaments and laver, which is applied in botany equipment and methods, horticulture, plant cultivation, etc., can solve the problems of not improving the spore release of laver shells, achieve low price, good spore release, and promote spore release Effect

Inactive Publication Date: 2016-07-27
NINGBO UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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  • Method for promoting diffusion of altar laver shell protonema conchospore
  • Method for promoting diffusion of altar laver shell protonema conchospore
  • Method for promoting diffusion of altar laver shell protonema conchospore

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Embodiment 1

[0026] The invention discloses a method capable of promoting the release of ascospores of shell filaments of algae porphyra. First, divide the shell filaments cultivated to maturity into several groups, each group of 16 shells is a string, hang them in a 1000ml beaker, add 600ml of common culture solution (naturally filtered seawater with a salinity of 25wt‰) , so that the water surface is not over the shell 1-2cm. Cultivate in an aerated incubator at 22±1°C, and in a shaker at 120rpm (or 40rpm or 300rpm) for 12-14h, and end the cultivation at 7:00 the next day, and put the shell filaments into 200ml of seawater culture solution During the process, the water surface was just submerged in the shells, and it was left to stand for 4 hours. After the spores were released, the spores were collected and the amount released was counted. It was found that if figure 1 As shown, the average release amount of conchospores under shaking table culture and aerated culture conditions was ...

Embodiment 2

[0028] The cultivation method is similar to Example 1, adopting the shaker cultivation method, the difference is that different concentrations of plant hormone salicylic acid and brassinolide are added to the culture solution. Fully dissolve salicylic acid (SIGMA company) in pure water to make the first mother liquor with a concentration of 1mol / L; dissolve 24-epibrassinolide (Shanghai Yuanye Biotechnology Co., Ltd.) in pure water to make Concentration is the second mother liquor of 1mol / L. After preliminary tests, the optimal final concentrations of salicylic acid were 200, 500umol / L; the optimal final concentrations of brassinosteroid were 4, 8umol / L, respectively. After the spores are released, the statistical results are as follows: figure 2 Effects of salicylic acid on the release of conchospores of Porphyra alba and image 3 The effect of brassinosteroids on the release of Porphyra japonica spores, all data are the average value of 4 times. The results showed that un...

Embodiment 3

[0030] The cultivation method is similar to Example 1, adopting the shaker cultivation mode. The difference is that different concentrations of plant hormones gibberellin and methyl jasmonate are added to the culture solution. Dissolve gibberellin (Shanghai Yuanye Biotechnology Co., Ltd.) in pure water to make the first mother solution with a concentration of 1mol / L; dissolve methyl jasmonate (Sia Reagent Company) in pure water to make a concentration of 1mol / L The second mother liquor of L. After preliminary tests, the optimal final concentrations of gibberellin were 4, 8umol / L, respectively; the optimal final concentrations of methyl jasmonate were 200, 500umol / L, respectively. After the spores are released, the statistical results are as follows: Figure 4 The effect of gibberellin on the release of conidia of Porphyra alba and Figure 5 The effect of methyl jasmonate on the amount of spore release of Porphyra alba, all the data are the average value of 4 determinations....

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Abstract

The invention discloses a method for promoting diffusion of altar laver shell protonema conchospore. The method is characterized by comprising the following steps that 1, brassinolide or gibberellin is dissolved in pure water, and mother liquor with the concentration of 1 mol/L is prepared; 2, the brassinolide mother liquor or the gibberellin mother liquor is added in a common culture solution to be prepared into a brassinolide culture solution with the concentration of 1-12 umol/L or a gibberellin culture solution with the concentration of 6-10 umol/L; 3, mature altar laver shell protonemata are suspended and completely immersed in the brassinolide culture solution or the gibberellin culture solution, then shake cultivation is carried out for 12 h to 14 h, cultivation is finished at 7:00 of the next day, then the mature altar laver shell protonemata are placed in seawater, standing is carried out for 2-10 hours, and altar laver conchospore is collected. The method has the advantages that the diffusion speed of the altar laver conchospore can be effectively increased, and meanwhile the diffusion quantity is remarkably increased.

Description

technical field [0001] The invention relates to a method for growing indoor filamentous seedlings of algae laver, in particular to a method for promoting the release of ascospores of shell filaments of algae laver. Background technique [0002] altar seaweed ( Pyropiahaitanensis ) belongs to the class Bangiophyceae (Bangiophyceae), Bangiaceae (Bangiaceae) economic seaweed, is a unique species of algae culture in my country, the main breeding areas are Guangdong, Fujian, Zhejiang and other coastal areas. Altar seaweed is delicious, high in protein, contains a large amount of essential amino acids, various minerals and vitamins, and is a high-quality nutritious food. It also has anti-aging, blood fat-lowering, and tumor-inhibiting effects. It is currently the seaweed with the greatest economic value. . The cultivation of altar laver includes two parts: indoor filamentous seedlings and offshore thallus cultivation. Among them, filamentous seedlings are the key to the success ...

Claims

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Application Information

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IPC IPC(8): A01G33/02
CPCA01G33/00
Inventor 侯赛男孙雪徐年军邹同雷
Owner NINGBO UNIV
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