Preparation method of muscle tendon anastomat containing growth factors

A growth factor and stapler technology, applied in the field of medical devices, can solve problems such as promotion of tendon anastomosis, limited effect of preventing adhesions, and complicated surgical operations, so as to promote endogenous healing, fibroblast proliferation, and mechanical strength. high effect

Inactive Publication Date: 2016-08-10
WUHAN UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The patented technology disclosed by Li Jin (Li Jin. Central tendon stapler, 2009.8) is a central tendon stapler that can provide sufficient mechanical strength for the postoperative tendon, so that patients do not need to brake after surgery. It can prevent tendon adhesion, but the operation is complicated and the effect of preventing adhesion is limited
Many published patent technologies have designed the shape of the tendon stapler, but have not studied its material, nor solved the problem of promoting tendon stapler

Method used

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  • Preparation method of muscle tendon anastomat containing growth factors
  • Preparation method of muscle tendon anastomat containing growth factors
  • Preparation method of muscle tendon anastomat containing growth factors

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] Dissolve polylactic acid-glycolic acid copolymer PLGA in dioxane with a mass percentage concentration of 2.5-3% and stir at a constant speed for 1-2 hours, pour the resulting solution on a plate, and place it overnight at -20°C; put in a mass ratio of 1 : 1.2~1.5 dehydrated ethanol extraction 8~10h, continuously 3 times, the gained agglomerate sample is put into freeze-drying machine freeze-drying, obtains the PLGA of freeze-extraction.

[0026] 1. Raw materials

[0027] (1) PLGA (70 / 30), 20g, with a molecular weight of about 150,000 to 200,000;

[0028] (2) bFGF, 10 mg;

[0029] (3) Dichloromethane, analytical purity ≥ 99.5%, 600ml.

[0030] 2. Preparation method

[0031] (1) Dissolving pretreated PLGA (70 / 30) in dichloromethane, the mass ratio of PLGA to dichloromethane is 1:30;

[0032] (2) Disperse the PBS solution containing the growth factor bFGF in the PLGA solution, the ratio is 1g PLGA contains 0.2mg bFGF, stir at a constant speed for 1 hour, pour it into a...

Embodiment 2

[0036] Dissolve polylactic acid-glycolic acid copolymer PLGA in dioxane with a mass percentage concentration of 2.5-3% and stir at a constant speed for 1-2 hours, pour the resulting solution on a plate, and place it overnight at -20°C; put in a mass ratio of 1 : 1.2~1.5 dehydrated ethanol extraction 8~10h, continuously 3 times, the gained agglomerate sample is put into freeze-drying machine freeze-drying, obtains the PLGA of freeze-extraction.

[0037] 1. Raw materials

[0038] (1) PLGA (90 / 10), 20g, with a molecular weight of about 150,000 to 200,000;

[0039] (2) bFGF, 10mg;

[0040] (3) Dichloromethane, analytical purity ≥ 99.5%, 600ml.

[0041] 2. Preparation method

[0042] (1) Dissolve the pretreated PLGA (90 / 10) in dichloromethane, and the mass ratio of PLGA to dichloromethane is 1:30;

[0043] (2) Disperse the PBS solution containing the growth factor bFGF in the PLGA solution, the ratio is 1g PLGA contains 0.25mg bFGF, stir at a constant speed for 1 hour, pour it ...

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Abstract

The invention belongs to the field of medical devices, and in particular relates to a preparation method of a tendon stapler containing growth factors. The polylactic acid-glycolic acid copolymer PLGA is pretreated by freezing extraction method and then mixed with a solution containing growth factors. The solvent was volatilized to obtain a PLGA film compounded with growth factors, which was hot-pressed to form a tubular tendon stapler. The advantages of the present invention are: in addition to providing sufficient mechanical strength for the tendon, avoiding the occurrence of secondary stretching, so that the patient does not need to brake after the operation, assists in rehabilitation training, and indirectly prevents the tendon from sticking to the surrounding tissue. In addition, it can also provide growth factors for tendon healing, promote fibroblast proliferation, and then promote endogenous tendon healing, prevent tendon adhesion, degradable absorption, and high mechanical strength.

Description

technical field [0001] The invention belongs to the field of medical devices, and in particular relates to a preparation method of a growth factor-containing tendon stapler. Background technique [0002] Tendon injury is a common clinical disease and a major problem in orthopedic surgery. Tendon tissue has the potential to heal itself. As long as the torn parts of the tendon can touch each other, and the tissues around the tendon sheath and paratendon are intact, the tendon can heal naturally. At present, the commonly used tendon surgical repair technique is tendon suture surgery, which mainly sutures the ruptured end of the tendon to make it butt joint, and uses the healing ability of the tendon itself to carry out tendon reconstruction. However, the traditional tendon suture technique has problems such as low mechanical strength, complicated operation, labor-intensive traction, inability to fix the tendon, and postoperative tendon adhesion. Tendon healing includes exogen...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L31/06A61L31/16A61B17/115
CPCA61L31/06A61B17/115A61L31/16A61L2300/252A61L2300/414
Inventor 张平赵芸万玲
Owner WUHAN UNIV OF TECH
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