Extraction and purification method of alfalfa polysaccharide

Abstract

The invention discloses an extraction and purification method of alfalfa polysaccharide. The method comprises the following steps: (1) soaking alfalfa powder in water for twice, combining filtrate obtained for twice, concentrating the filtrate and then carrying out alcohol precipitation, collecting precipitates, then centrifuging or sieving the precipitates, collecting the precipitates, then adding water to dissolve the precipitates and prepare an alfalfa polysaccharide solution; and (2) successively carrying out dialysis and deproteinization treatment on the alfalfa polysaccharide solution prepared in the step (1), precipitating a system prepared by deproteinization, then centrifuging or sieving the system, collecting supernatant, carrying out alcohol precipitation, collecting the precipitates and drying the precipitates to obtain the alfalfa polysaccharide. Through the extraction and purification method of alfalfa polysaccharide, a conventional water-boiling and alcohol-extracting method of alfalfa polysaccharide is improved; the dialysis process is added; the sample is subjected to qualitative and quantitative detection by alpha-naphthol experiment, Fehling experiment, ninhydrin experiment, ferric trichloride experiment and anthranone-sulfuric acid colorimetry; the result shows that the extraction rate of the improved method reaches up to 89.87 percent.

Description

technical field [0001] The invention belongs to the field of agriculture and relates to a method for extracting and purifying polysaccharides from alfalfa. Background technique [0002] According to the survey report on the use of antibiotics in livestock and poultry farming in China published in June 2013, the breeding industry is currently the field with the largest amount of antibiotic use. The total domestic production of antibiotics is 210,000 tons, and the domestic consumption is about 180,000 tons, of which 97,000 tons are used in animal husbandry and its feed industry, accounting for about 54%. Extensive use of antibiotics has led to increased drug resistance in animals and weakened disease resistance in livestock and poultry; long-term use of antibiotics has led to imbalances in the intestinal flora of the body and destroyed the micro-ecological environment; at the same time, drug residues in animal products have become a prominent problem. In 1986, Sweden became t...

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Problems solved by technology

Extensive use of antibiotics has led to increased drug resistance in animals and weakened disease resistance in livestock and poultry; long-term use of antibiotics has led t...

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