A kind of extraction and purification method of alfalfa polysaccharide

Abstract

The invention discloses a method for extracting and purifying alfalfa polysaccharide. The method comprises: 1) leaching the alfalfa powder twice with water, combining the filtrate obtained twice, concentrating, ethanolizing, collecting the precipitate, centrifuging or sieving, collecting the precipitate, adding water to dissolve the precipitate, and obtaining crude alfalfa polysaccharide solution; 2) dialyze and deproteinize the crude alfalfa polysaccharide solution obtained in step 1) sequentially, centrifuge or sieve the obtained system after deproteinization, collect the supernatant, and after alcohol analysis, collect the precipitate and dry to obtain the Alfalfa polysaccharides. The present invention improves the traditional boiled alcohol extraction method of alfalfa polysaccharides, increases the dialysis process, and passes the α-naphthol experiment, the Fehling experiment, the ninhydrin experiment, the ferric chloride experiment and the anthrone-sulfuric acid colorimetric method The samples were tested qualitatively and quantitatively, and it was found that the purity of the alfalfa polysaccharide obtained by the improved method was as high as 89.87%.

Description

technical field [0001] The invention belongs to the field of agriculture and relates to a method for extracting and purifying polysaccharides from alfalfa. Background technique [0002] According to the survey report on the use of antibiotics in livestock and poultry farming in China published in June 2013, the breeding industry is currently the field with the largest amount of antibiotic use. The total domestic production of antibiotics is 210,000 tons, and the domestic consumption is about 180,000 tons, of which 97,000 tons are used in animal husbandry and its feed industry, accounting for about 54%. Extensive use of antibiotics has led to increased drug resistance in animals and weakened disease resistance in livestock and poultry; long-term use of antibiotics has led to imbalances in the intestinal flora of the body and destroyed the micro-ecological environment; at the same time, drug residues in animal products have become a prominent problem. In 1986, Sweden became t...

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Problems solved by technology

Extensive use of antibiotics has led to increased drug resistance in animals and weakened disease resistance in livestock and poultry; long-term use of antibiotics has led to imbalances in the intestinal flora of the body and destroyed the micro-ecological environment; at the same time, drug residues in animal products have become a prominent problem

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