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Method for preparing yeast fusants and screening glutathione high-yield strains

A yeast fusogen, glutathione technology, applied in the field of applied microorganisms, to achieve the effects of high GSH yield, fast growth rate and stable genetics

Active Publication Date: 2016-08-10
SHAANXI UNIV OF SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, in order to meet the needs of industrial production, the GSH yield and ethanol tolerance of the strain still need to be further improved to achieve the purpose of efficient GSH production, and it is difficult to improve the GSH yield and ethanol tolerance only by mutagenesis

Method used

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  • Method for preparing yeast fusants and screening glutathione high-yield strains

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Embodiment Construction

[0031] The present invention will be described in detail below in conjunction with the accompanying drawings and embodiments.

[0032] (1) Technical route and starting strains, culture medium and reagents

[0033] In the present invention, Hansenula polymorpha strain and Schizosaccharomyces pombe strain are used as starting strains (parents), and cell fusion technology is used to integrate the superior properties of the two parental yeast strains, so as to realize the improvement of the superior properties of the fusion son, and then improve the production of GSH efficiency.

[0034] Bacterial strains that the present invention relates to:

[0035] Hansenula polymorpha (Hansenula polymorpha) DL-18, by the applicant himself in the Chinese patent "Hansenula polymorpha mutant strain and its application in glutathione biosynthesis" (publication number CN103060210A, publication date: 2013.4 .24) disclosed in; Schizosaccharomyce spombe strain (S.pombe 2.1794), purchased from Insti...

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Abstract

The invention provides a method for preparing yeast fusants and screening glutathione high-yield strains. The method comprises the following steps: respectively preparing protoplasts of hansenula polymorpha and schizosaccharomyces pombe; fusing the protoplasts to obtain fusion processing cells; performing high temperature resistance, ethanol tolerance and sodium molybdate tolerance screening on the fusion processing cells to obtain the glutathione high-yield strains. According to the method, the fusants of the high-yield glutathione can be screened efficiently; the fusants obtained by screening have the advantages of high temperature resistance, high growth rate, genetic stability, high yield of the glutathione, and the like; the strains with application value are provided for the large-scale production of the glutathione.

Description

technical field [0001] The invention belongs to the field of applied microorganisms, and in particular relates to a method for preparing yeast fusions and screening high glutathione-producing strains from yeast fusions. Background technique [0002] Glutathione (GSH) is an active tripeptide composed of glutamic acid, cysteine ​​and glycine, which exists in almost all cells of organisms. Since the active group thiol on cysteine ​​can combine with toxins such as heavy metals, it has an integrated detoxification effect, and also has an antioxidant effect, thereby delaying aging, that is, GSH has a broad-spectrum detoxification effect. In addition, GSH also has a certain effect on the immune system, which can effectively maintain the function of the immune system and enhance immunity. Therefore, GSH is widely used in the fields of medicine, health food and cosmetics. [0003] At present, the domestic market demand for GSH is increasing day by day, but my country's GSH is almos...

Claims

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Application Information

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IPC IPC(8): C12N15/04C12N15/01C12N13/00C12R1/78C12R1/645
CPCC12N13/00C12N15/01C12N15/04
Inventor 钱卫东谢海艳吴启航
Owner SHAANXI UNIV OF SCI & TECH
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