Polypeptide group for identification of donkey components in gelatin and products thereof,

A technology of donkey-derived and donkey-hide gelatin is applied in the field of identifying donkey-derived components of donkey-hide gelatin and its products.

Active Publication Date: 2016-08-10
INSPECTION & QUARANTINE TECH CENT SHANDONG ENTRY EXIT INSPECTION & QUARANTINE BUREAU
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Part of the reason for the repeated prohibition of donkey-hide gelatin fraud is the lack of technical support for identification. After the animal skin is boiled and dissolved, its own characteristics are destroyed, and it is difficult to identify the source of t

Method used

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  • Polypeptide group for identification of donkey components in gelatin and products thereof,
  • Polypeptide group for identification of donkey components in gelatin and products thereof,
  • Polypeptide group for identification of donkey components in gelatin and products thereof,

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0141] Embodiment 1, screening donkey donkey-hide gelatin collagen-specific polypeptide

[0142] The pure donkey collagen samples and bovine collagen samples were analyzed by mass spectrometry, and the mass spectrometry results were searched and compared using ProteinPilot software to determine the most preferred donkey collagen-specific peptide sequence.

[0143] First, perform mass spectrometry analysis on the selected pure collagen sample, the steps include:

[0144] (1) Sample pretreatment steps:

[0145] (1) Weigh 0.1g ~ 0.5g of pure sample homogenized into powder state, add 1% NH 4 HCO 3 solution, sonicate for 10-30 minutes to dissolve the sample completely, and dilute to 50mL;

[0146] (2) The solution is passed through a 0.22 μm filter membrane;

[0147] (3) Take 10-20 μL Trypsin enzyme solution (1 μg / μL Trypsin enzyme solution) and add it to 100 μL-200 μL of the above filtrate, enzymatically hydrolyze at 37°C for 16-18 hours, and wait for detection on the machine....

Embodiment 2

[0232] Example 2: Detect unspecific donkey-hide gelatin samples to determine whether they contain donkey collagen

[0233] Mass spectrometry analysis was carried out on the donkey-hide gelatin boiled from animal skin and its product samples and the commercially available donkey-hide gelatin products submitted by the public security bureau of a certain city to determine whether it contained donkey collagen.

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Abstract

The present invention relates to a polypeptide group for identification of donkey components in gelatin and products thereof, and a mass spectrometry method to identify the donkey derived components in gelatin and products thereof by using the polypeptide. The polypeptides in the polypeptide group has a specific sequence structure and a specific m/z value (including a specific parent ion and a pair of daughter ions), and the sequence is shown in SEQIDNO.1-35; and the peptide segment and its m / z are specific in donkey source gelatin and are not seen in bovine gelatin.

Description

technical field [0001] The invention belongs to the field of biotechnology, and specifically relates to a group of polypeptides for identifying donkey-derived components in donkey-hide gelatin and its products and a method for using the same. Background technique [0002] Donkey-hide gelatin is a solid gelatin made from the skin of an equine animal donkey, which is boiled and concentrated. It was originally produced in Dong'e County, Shandong Province, and has a history of nearly 3,000 years. Donkey-hide gelatin is a traditional top-grade nourishing and blood-enriching medicine. It tastes sweet and flat. It enters the lung, liver, and kidney meridians. , Anti-fatigue, improve immunity, suitable for a wide range of people. Li Shizhen's "Compendium of Materia Medica" contains: "Ejiao's "Benjing" is top grade. Hongjing said: 'It came out of Dong'e, so it is named Ejiao'". Donkey-hide gelatin is a typical Chinese medicinal material that pays attention to "authenticity". Authen...

Claims

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Application Information

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IPC IPC(8): G01N30/88
CPCG01N30/88
Inventor 张鸿伟张晓梅梁成珠鲍蕾徐彪
Owner INSPECTION & QUARANTINE TECH CENT SHANDONG ENTRY EXIT INSPECTION & QUARANTINE BUREAU
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