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Method and device for constructing animal model suffering from diabetes induced by high uric acid content

An animal model and construction method technology, which is applied in animal houses, applications, animal husbandry, etc., can solve the problems of low molding rate of animal models, solve the problem of artificial breeding, speed up the induction time, and shorten the modeling period. Effect

Inactive Publication Date: 2016-08-17
THE AFFILIATED HOSPITAL OF QINGDAO UNIV +1
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, the animal model prepared by the preparation method of the type II diabetes animal model has a low modeling rate and can only be applied to the clinical research of diabetes

Method used

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  • Method and device for constructing animal model suffering from diabetes induced by high uric acid content
  • Method and device for constructing animal model suffering from diabetes induced by high uric acid content
  • Method and device for constructing animal model suffering from diabetes induced by high uric acid content

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Embodiment 1

[0056] According to Embodiment 1 of the present invention, a method for constructing an animal model of diabetes induced by high uric acid is provided, comprising the following steps.

[0057] Ten 10-week-old Wistar male rats of 220g-260g were prepared in advance, fed with common feed for one week and then modeled.

[0058] In step S1, the first modeling stage is carried out as follows every day: feed the rats with common feed, and gavage the rats with adenine aqueous solution at 9 o'clock in the morning. The adenine aqueous solution contains 4% adenine by mass percentage. Purine, the amount of adenine aqueous solution is set to be about 110 mg of adenine per kilogram of target animal body weight per day (prepare about 1 ml of adenine aqueous solution for each rat to ensure the actual intake), 3 p.m. At one o'clock, rats were subcutaneously injected once with ethambutol aqueous solution, which contained 5% ethambutol in terms of mass percentage, and the amount of ethambutol aq...

Embodiment 2

[0067] Except that Wistar male rats were adjusted to SD rats, other conditions were the same as in Example 1.

Embodiment 3

[0069] Except for the following aspects, other conditions are the same as in Example 1: in the first modeling stage, the adenine aqueous solution contains 5% adenine in terms of mass percentage, and the consumption of the adenine aqueous solution is set to be 5% per kilogram of target animal body weight per day. The dose of adenine is about 120 mg; the ethambutol aqueous solution contains 3% ethambutol by mass percentage, and the amount of ethambutol aqueous solution is set to the daily intake of ethambutol per kg of target animal body weight The dosage is 200 mg. In the intermediate modeling stage, the streptozotocin preparation solution contains 0.5% streptozotocin in terms of mass percentage, and the amount of streptozotocin preparation solution is set to be the daily intake of streptozotocin per kilogram of target animal body weight The dose of Zotocin is 30 mg. In the second modeling stage, the adenine aqueous solution contains 3% adenine in terms of mass percentage, and...

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Abstract

The invention discloses a method and device for constructing an animal model suffering from diabetes induced by the high uric acid content. The method comprise the steps of 1, conducting the following operation at the first stage, wherein a target animal is fed with ordinary high-fat feed, one or more times of gavage is conducted on the target animal with an adenine aqueous solution, and one or more times of subcutaneous injection is conducted on the target animal with an ethambutol aqueous solution; 2, conducting the following operation at the second stage, wherein the target animal is fed with ordinary high-fat feed, gavage is conducted on the target animal with an adenine aqueous solution, and subcutaneous injection is conducted on the target animal with an ethambutol aqueous solution. The first modeling stage lasts for 5-7 weeks, and the second modeling stage lasts for 7-9 weeks.

Description

technical field [0001] The invention relates to a model for disease research, in particular to a method and device for constructing an animal model for medical research. Background technique [0002] With the improvement of people's living standards, the structure of life tends to be modernized, and the population structure tends to age, the prevalence of hyperuricemia is increasing, and it has become a common and frequently-occurring disease. Hyperuricemia is a purine metabolic disorder and one of the diseases closely related to metabolic syndrome and diabetes. It is mainly caused by excessive uric acid or decreased renal excretion. [0003] Sustained hyperuricemia can easily lead to gout—causing joint pain, and easily involving the kidneys and cardiovascular system, seriously endangering human health. Uric acid is the catabolism product of purine nucleotides in the human body. 80% of purine nucleotides are produced by the metabolism of human cells, and 20% are obtained fr...

Claims

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Application Information

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IPC IPC(8): A01K67/02A01K1/03A01K1/035
CPCA01K1/031A01K1/0356A01K67/02
Inventor 邢士超李长贵张少燕牛佳鹏任伟苗志敏
Owner THE AFFILIATED HOSPITAL OF QINGDAO UNIV
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