Boc-L-valine pharmaceutical composition and application in biological pharmacy
A technology of boc-l- and valine, which is applied in the field of biomedicine and can solve problems that have not been reported yet
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Embodiment 1
[0018] Example 1: Compound (I) Separation Preparation and Structure Confirmation
[0019] Separation method: (a) crush the dried lily bulbs (2kg), extract with 90% ethanol under heat reflux (15L × 3 times), combine the extracts, concentrate until no alcohol smell (3L), and then use petroleum ether (3L× 3 times), ethyl acetate (3L × 3 times) and water-saturated n-butanol (3L × 3 times) were extracted to obtain petroleum ether extract, ethyl acetate extract and n-butanol extract respectively; (b) step (a) The ethyl acetate extract in (a) was removed with D101 type macroporous resin, first eluted with 35% ethanol for 8 column volumes, then with 90% ethanol for 12 column volumes, collected 90% eluent, and subtracted Concentrate under pressure to obtain the 90% ethanol elution concentrate; (c) 90% ethanol elution concentrate in step (b) is separated with normal phase silica gel, and the volume ratio is 120:1 (11 column volumes), 60:1 successively (9 column volumes), 30:1 (9 column...
Embodiment 2
[0022] Embodiment 2: pharmacological action
[0023] 1. Materials and methods
[0024] 1.1 Animals
[0025] Healthy male clean-grade SD rats with a body weight of 320-370 g (provided by the Animal Experiment Center of Nanjing Military Region General Hospital) were selected. The animals were kept in a ventilated and dry environment with a temperature of (22±1)°C, a relative humidity of 55%-65%, and a 12-h light cycle, and were fed with maintenance diet 1022 for rats and mice.
[0026] 1.2 Reagents and samples
[0027] Boc-L-valine was purchased from China Institute for the Control of Pharmaceutical and Biological Products. Compound (I) is self-made, and the preparation method is shown in Example 1. Rat tumor necrosis factor-α (TNF-α), interleukin-6 (IL-6), interleukin-10 (IL-10), enzyme-linked immunosorbent assay (ELISA) detection kits were purchased from Biosource, USA International Company; rat HMGB1 ELISA detection kit was purchased from Japan Shino-Test Company. HMGB1...
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