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Enterobacter ludwigii with quinclorac degradation function and application thereof

A technology of Enterobacter ludwig and Enterobacter dewighi, applied in the field of degrading quinclorac, can solve problems such as adverse effects on the growth and quality of flue-cured tobacco, economic loss in tobacco leaf production, etc., and achieve high tolerance and degradation ability , strong vitality, fast growth effect

Inactive Publication Date: 2016-08-17
BEIJING HEHE RUNSHENG TECH CO LTD +2
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Therefore, quinclorac-based pesticide residues in the soil have adverse effects on the growth and quality of flue-cured tobacco, and will bring serious economic losses to tobacco leaf production.

Method used

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  • Enterobacter ludwigii with quinclorac degradation function and application thereof
  • Enterobacter ludwigii with quinclorac degradation function and application thereof
  • Enterobacter ludwigii with quinclorac degradation function and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0045] This example is used to illustrate the tolerance and degradability of Enterobacter ludwigii provided by the present invention to quinclorac in water

[0046] The strain LZ-1 was inserted into the LB liquid medium containing 10mg / L, 20mg / L, 50mg / L, 100mg / L, 200mg / L quinclorac after sterilization with an inoculum of 1% by volume. middle. Cultured at 170rpm and 30±1℃ for 24h, the results showed that the strain LZ-1 showed good tolerance to quinclorac, and the strains could grow in different media. The maximum OD value that the bacteria can reach and the degradation rate of quinclorac are shown in Table 1.

Embodiment 2

[0048] This example is used to illustrate the tolerance and degradability of Enterobacter ludwigii provided by the present invention to quinclorac in water

[0049] The strain LZ-1 was inoculated into the inorganic salts of quincloracic acid with concentrations of 10mg / L, 20mg / L, 50mg / L, 100mg / L and 200mg / L after sterilization with an inoculum of 1% by volume. base. Incubate at 170rpm and 30±1℃ for 24h. The results showed that the strain LZ-1 showed good tolerance to quinclorac, and the strains could grow in different media. In the case of taking quinclorac as the only carbon source, the maximum OD value that the bacteria can reach and the degradation rate of quinclorac are shown in Table 1.

Embodiment 3

[0057] This example is used to illustrate the degradation of quinclorac in soil by Enterobacter ludwigii provided by the present invention

[0058] The strain LZ-1 was inoculated into 100 mL of LB liquid medium at 1% by volume, and after culturing at 175 rmp for 24 h at 30 ± 1 °C, the bacterial liquid was added to 5 kg containing 20 mg / kg of quinclorac. In the soil, stir evenly and cultivate for 7 days to ensure that the water content in the soil is about 20%. Finally, the content of quinclorac in the soil is detected. The results are shown in Table 2.

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Abstract

The present invention discloses Enterobacter ludwigii with preservation No. CGMCC No. 10128. The invention also discloses a composition containing the Enterobacter ludwigii live bacteria described above. The present invention further discloses the application of above Enterobacter ludwigii and compositions in the degradation of quinclorac. The present invention also discloses a method for degrading quinclorac. The method comprises the steps of: under the conditions suitable for Enterobacter ludwigii to survive, adding the above Enterobacter ludwigii or composition into an environment containing quinclorac in order to degrade quinclorac. The Enterobacter ludwigii simply requires the conditions of carbon source, nitrogen source and inorganic salts to survive and grow in an atmosphere containing quinclorac, has the advantages of strong vitality, fast growth, large biomass and high tolerance and degradation ability to quinclorac.

Description

technical field [0001] The present invention relates to a strain of Enterobacter ludwigii (Enterobacter ludwigii), in particular, to a strain of Enterobacter ludwigii with quinclorac acid degradation function and application thereof, and a strain of Enterobacter ludwigii containing the Enterobacter ludwigii Compositions and methods of degrading quinclorac. Background technique [0002] Quinolinic acid (3,7-dichloro-8-quinoline carboxylic acid) is a highly selective hormone-type herbicide developed by BASF in Germany. It is widely used in many rice-growing countries to control monocotyledonous and dicotyledonous leaves Leaf weeds, especially for the control of barnyardgrass in paddy fields, have good herbicidal effect and long residual period, and are more and more widely used. Due to the lack of sufficient understanding of the herbicidal properties of quinclorac, blind application and increased dosage will result in soil residues causing phytotoxicity to the next crop, and ...

Claims

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Application Information

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IPC IPC(8): C12N1/20B09C1/10C02F3/34C12R1/01C02F101/38
Inventor 过钰梁林森张飞燕卢滇楠张杰刘铮
Owner BEIJING HEHE RUNSHENG TECH CO LTD
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