A kind of high-efficiency erosion bacterium Serratia liquefaction nlx-15 of silicate rock and its application

A technology of Serratia and silicate, applied in the field of microorganisms, to achieve the effects of accelerated erosion into soil, strong erosion ability, and good application and development prospects

Active Publication Date: 2019-06-11
NANJING FORESTRY UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there is no report on the screening of suitable efficient rock-eroding microbial strains for different rocks in China

Method used

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  • A kind of high-efficiency erosion bacterium Serratia liquefaction nlx-15 of silicate rock and its application
  • A kind of high-efficiency erosion bacterium Serratia liquefaction nlx-15 of silicate rock and its application
  • A kind of high-efficiency erosion bacterium Serratia liquefaction nlx-15 of silicate rock and its application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] The acquisition and identification of embodiment 1 bacterial strain:

[0022] Collect the colonies in the biological crust on the bare rock surface of silicate rock (Jiangxi Lushan rock mining area), and use the dilution plate method to screen the medium (5.0g sucrose, 2.0gNa 2 HPO 4 , 0.5gAlSO 4 ·7H 2 O, 0.005g FeCl 3 , 0.1 g CaCO 3 , 1.0 Silicate rock slag (cleaned Lushan rock mining area rock, polished to rock powder, 200 mesh sieve, main chemical composition: 73.8% SiO 2 , 13.4% Al 2 o 3 , 9.9%K 2 (2), 18g agar powder, 1000mL distilled water) were cultivated for about 48 hours, single colonies were picked, streaked and purified repeatedly, and the resulting bacterial strains were respectively inoculated on the slant of beef extract peptone solid medium, stored in a refrigerator at 4°C to obtain pure bacterial strains. The main biological characteristics of the strain: cultured at 28°C on beef extract peptone solid medium, the thalline is straight rod-shaped,...

Embodiment 2

[0024] The silicate rock dissolution ability of embodiment 2 bacterial strains:

[0025] Use an inoculation loop to inoculate a small amount of pure Serratia liquefaction strain NLX-15 into a 250mL Erlenmeyer flask filled with 50mL screening medium (the medium is the same as above, without adding agar powder), culture at 28°C and shake at 180r / min for 72h, and obtain Activate the bacteria.

[0026] Take 2.0mL of bacterial suspension cultured for 24h (6.0×10 6 cfu mL -1 ) into 100mL potassium-deficient medium (10g sucrose, 2.0g NaHPO 4 , 0.5g AlSO 4 ·7H 2 O, 0.1g NaCl, 0.5g yeast extract, 1000mL deionized water, adjust the pH to 7.2-7.4. ) and 0.2g of silicate rock powder in a 250mL Erlenmeyer flask, another 1mL of high-temperature inactivated bacterial suspension at 121°C was inserted into the culture medium as a control, and three parallel samples were made for each treatment method. Culture at 28°C with shaking at 180rpm. Take 5.0mL of the fermentation broth cultured ...

Embodiment 3

[0035] The influence of embodiment 3 bacterial strains on plant growth

[0036] After the bacterial strain is activated (the method is the same as in Example 2), according to 100mL·kg -1 Mix in the spraying matrix (woodland soil, add humic acid organic fertilizer 25%, silicate rock powder (200 mesh sieve) 4%, spraying wood fiber 0.3%, water retaining agent 0.3%, greening spraying adhesive 0.03% ), and the control was mixed with the same volume of culture medium in the spraying substrate, and carried out a pot test (pot diameter 17cm, depth 15cm). Place them in a greenhouse at 25°C. After the seeds germinate, keep the three best seedlings in each pot, and measure the height and biomass of the plants after 3 months. , and each experiment was done in 3 parallel treatments.

[0037] Plant height is one of the most basic indicators in the investigation of plant morphology, which refers to the distance from the root neck to the top of the plant. It can be seen from Table 2 that t...

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Abstract

The invention discloses a silicate rock high-efficiency corrosion bacterium Serratia liquefaction NLX-15 and application thereof. The efficient erosion bacteria of silicate rock, its taxonomic name is Serratia liquefactionus Serratia liquefaciens NLX‑15 has been deposited in the China Center for Type Culture Collection, accession number CCTCC NO: M2016239, preservation date: May 3, 2016; preservation address: Wuhan University, Wuhan, China. Serratia liquefaction NLX‑15, an efficient rock erosion bacterial strain screened out by the present invention, has a strong erosion ability to silicate rocks, can promote the release of major ions such as Ca and Al in rocks, and accelerate the erosion of limestone into soil; at the same time It also has a strong potential to decompose K and P; at the same time, it can promote the growth of plants after spraying, and has a good application and development prospect.

Description

technical field [0001] The invention belongs to the technical field of microbes, and in particular relates to Serratia liquefactionus NLX-15, a silicate rock high-efficiency corrosion bacterium, and an application thereof. Background technique [0002] The guest soil spraying technology is a greening technology that sprays the mixture of plant seeds and growth matrix materials evenly and at high pressure on the rock and soil slope. This technology is mainly a greening technology developed for hard slopes such as rocks. At present, it has been widely used in slope greening of water conservancy, highway, railway and other projects. However, this technology must meet two conditions for the greening of rock slopes: first, there is a planting substrate on which plants can continue to grow; erosion. However, in actual engineering, the current guest soil spraying technology is difficult to solve the above two technical problems, and the spraying matrix is ​​difficult to maintain...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20A01N63/00A01P21/00C09K17/14C12R1/425C09K101/00
CPCA01N63/00C09K17/14C09K2101/00C12N1/205C12R2001/425
Inventor 张金池吴雁雯张波王凌剑
Owner NANJING FORESTRY UNIV
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