Compound alga oligosaccharide microbial fertilizer and preparation method thereof

A technology of compound microbial fertilizer and seaweed oligosaccharides, applied in the direction of microorganism-based methods, biochemical equipment and methods, microorganisms, etc., can solve the problem that the effect of plant growth promotion is not reflected, affects the effect of seaweed oligosaccharides, and the molecular weight of seaweed oligosaccharides There is a big difference between the problems, to achieve the effect of strong salt tolerance, strong plant growth promotion ability, and good growth

Inactive Publication Date: 2016-08-24
FUJIAN SANJU BIOLOGICAL SCI & TECH
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Problems solved by technology

[0005] Chinese patent CN201410359147.X discloses a preparation method of seaweed oligosaccharide bio-fertilizer, inoculate Flammeovirga Pacifica H2 into the sugar production medium, centrifuge after fermentation, collect the supernatant, and pass the supernatant through a tube After membrane filtration, the filtrate is collected and treated with protease to obtain seaweed oligosaccharide biofertilizer; the seaweed oligosaccharide biofertilizer can significantly increase the content of available nitrogen, phosphorus, potassium and soil organic matter in the soil, and promote the growth of Flavobacterium in the soil , but the growth-promoting effect on plants has not been reflected
[0007] At present, most seaweed oligosaccharide biofertilizers are compounded with macroelements and / or medium and trace elements, and the molecular weights of seaweed oligosaccharides used vary greatly, which affects the effect of seaweed oligosaccharides
Moreover, most of them are mainly brown algae oligosaccharides and green algae oligosaccharides, while red algae oligosaccharides, especially the combination of asparagus oligosaccharides with a degree of polymerization of 4-8 and functional bacteria, have not been reported in microbial fertilizers. and research

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  • Compound alga oligosaccharide microbial fertilizer and preparation method thereof
  • Compound alga oligosaccharide microbial fertilizer and preparation method thereof
  • Compound alga oligosaccharide microbial fertilizer and preparation method thereof

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0034] The preparation of embodiment 1 seaweed oligosaccharide compound microbial fertilizer

[0035] 1) Use solid nitrogen-free medium (sucrose 5g / L, dipotassium hydrogen phosphate 0.5g / L, magnesium sulfate 0.1g / L, sodium chloride 0.2g / L, calcium carbonate 0.6g / L) to jelly-like bacillus , agar 20g / L, distilled water configuration, pH value before sterilization is 7.0, 121 ℃, 20min after sterilization), after activation, inoculate into seed medium (10g / L, dipotassium hydrogen phosphate 0.8g / L, sulfuric acid Magnesium 0.4g / L, sodium chloride 0.35g / L, calcium carbonate 1.0g / L, distilled water configuration, pH value before sterilization is 7.2, 121℃, 20min after sterilization), at 30℃, 200rpm Shake culture for 24 hours to obtain a primary seed fermentation liquid; inoculate the primary seed liquid into a seed fermenter, keep the stirring speed of the fermenter at 150 rpm at 30°C, and cultivate for 36 hours to obtain a secondary seed liquid.

[0036] 2) Inoculate the secondary s...

Embodiment 2

[0039] The experimental design consists of 7 experimental groups, namely:

[0040] Negative control group CK: clear water;

[0041] Positive control group CK1: seaweed oligosaccharide solution;

[0042] Positive control group CK2: fermentation broth of Bacillus jelly-like;

[0043] Test group A: seaweed oligosaccharide compound microbial fertilizer Ⅰ;

[0044] Test group B: seaweed oligosaccharide compound microbial fertilizer II;

[0045] Test group C: seaweed oligosaccharide compound microbial fertilizer III;

[0046] Test group D: seaweed oligosaccharide compound microbial fertilizer IV.

[0047] The soil was sieved to remove sundries, stones, etc., and mixed well to make a test soil sample, with 2kg of soil in each pot. Conventional fertilization, with urea 0.7g / kg, KH 2 PO 4 0.5g / kg, KCl 0.085g / kg addition amount to add nitrogen, phosphorus and potassium fertilizer solution to the soil sample. Select rape seeds with plump grains and the same size and disinfect th...

Embodiment 3

[0056] The soil was sieved to remove sundries, stones, etc., and mixed well to make a test soil sample, with 2kg of soil in each pot. Conventional fertilization, with urea 0.7g / kg, KH 2 PO 4 0.5g / kg, KCl 0.085g / kg addition amount to add nitrogen, phosphorus and potassium fertilizer solution to the soil sample. Select lettuce seeds with plump grains and the same size and disinfect them with 1% sodium hypochlorite for 15 minutes, rinse them with deionized water, and then soak them in deionized water for 12 hours. The lettuce seeds soaked in water are sown in pots, and after one week of germination, seedlings with the same growth are selected for seedling setting, 5 plants in each pot, sprayed and watered, so that the field water holding capacity is about 60% to 70%.

[0057] The seaweed oligosaccharide compound microbial fertilizer of the above test group was diluted 800 times with water. Take 4mL, 6mL, 8mL, and 10mL of the above-mentioned diluted seaweed oligosaccharide com...

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Abstract

The invention provides a compound alga oligosaccharide microbial fertilizer and a preparation method thereof and relates to a microbial fertilizer. The compound alga oligosaccharide microbial fertilizer is prepared from, by volume, an alga oligosaccharide solution 20%-80% and bacillus mucilaginosus fermented liquid 20%-80%, wherein the effective number of living bacteria is greater than or equal to 0.2 billion/mL, the alga oligosaccharide content is greater than or equal to 0.5 g/kg, and a pH value is 6.8-7.2. The preparation method comprises the steps that bacillus mucilaginosus is activated by using a solid nitrogen-free culture medium and then is inoculated to a seed culture medium to obtain primary seed fermentation liquid; the primary seed fermentation liquid is inoculated to a seed fermentation tank, and culture is performed to obtain secondary seed liquid; the second seed liquid is inoculated to a fermentation culture medium, and fermentation culture continues to prepare the bacillus mucilaginosus fermented liquid; the alga oligosaccharide solution and the bacillus mucilaginosus fermented liquid are mixed to obtain the compound alga oligosaccharide microbial fertilizer. The compound alga oligosaccharide microbial fertilizer has the effects of improving soil, improving fertilizer efficiency, adjusting crop growth, improving crop quality and the like.

Description

technical field [0001] The invention relates to microbial fertilizers, in particular to a seaweed oligosaccharide compound microbial fertilizer and a preparation method thereof. Background technique [0002] On March 18, 2015, the Ministry of Agriculture of China issued the "Action Plan for Zero Growth in Fertilizer Use by 2020". With the cancellation of preferential policies such as fertilizer production electricity charges and value-added tax, the fertilizer industry is changing from special commodities to general commodities; nitrogen fertilizer, The overcapacity of phosphate fertilizers and the low profit level of the chemical fertilizer industry as a whole are facing the difficulties of decapacity reduction and structural adjustment, which brings opportunities for microbial fertilizers. The country has retained the preferential policy of exempting value-added tax from the production and circulation of microbial fertilizers, which means that the application of microbial ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C05F11/08C12N1/20C12R1/07
CPCC05F11/00C05F11/08C12N1/20
Inventor 余劲聪何舒雅林克明尤越
Owner FUJIAN SANJU BIOLOGICAL SCI & TECH
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