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A method for preparing low-ester pectin by ammonium exchange method

A low-ester pectin and high-ester pectin technology, which is applied in the field of food processing, can solve the problems of high equipment requirements, high production costs, and high requirements for supporting auxiliary equipment, and achieve simple process flow, mild reaction conditions, and low production costs. Effect

Active Publication Date: 2018-05-25
HUAZHONG AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Among them, the alkali hydrolysis method cannot accurately control the degree of esterification of pectin, and as the alkali hydrolysis time prolongs, the degree of esterification of pectin decreases while the molecular chain of pectin also breaks, which significantly reduces the physical and chemical properties of pectin; high static pressure pectin A The esterase method has relatively high requirements on the type of enzyme, and requires high static pressure equipment to assist, and the production cost is high; the water extraction method has high requirements on equipment; the microbial enzymatic hydrolysis method has high requirements on the type of microorganisms and supporting auxiliary equipment

Method used

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  • A method for preparing low-ester pectin by ammonium exchange method
  • A method for preparing low-ester pectin by ammonium exchange method
  • A method for preparing low-ester pectin by ammonium exchange method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] 1) Add 3g of pectin powder to a 100ml Erlenmeyer flask, then add distilled water to 100ml, shake to completely dissolve the pectin.

[0026] 2) When the pectin is completely dissolved, add 7ml of 25g / L ammonium acetate solution, and stir at 40°C for 24h.

[0027] 3) Use hydrochloric acid to adjust the pH of the solution to 2, then add 2 times the volume of absolute ethanol, stir evenly, filter to obtain pectin precipitate, dry with hot air at 40°C to constant weight, and pulverize to obtain low-ester pectin.

[0028] The degree of esterification of low-ester pectin was determined to be 43% by titration. After the sample was dried, the composition of the sample was analyzed by infrared spectroscopy and compared with a low-ester pectin standard, such as figure 1 and figure 2 . The results showed that the absorption peaks of the samples were basically consistent with those of the standard substance, indicating that this method only reduced the degree of esterification ...

Embodiment 2

[0030] 1) Add 2g of pectin powder into a 100ml Erlenmeyer flask, then add distilled water to 100ml, shake to completely dissolve the pectin.

[0031] 2) When the pectin is completely dissolved, add 4.5ml of 20g / L ammonium acetate solution, and stir at 70°C for 1h.

[0032] 3) Use hydrochloric acid to adjust the pH of the solution to 1, then add 3 times the volume of absolute ethanol, stir evenly, filter to obtain pectin precipitate, dry with hot air at 50°C to constant weight, and pulverize to obtain low-ester pectin.

[0033] The esterification degree of gained low-ester pectin is 49%, and infrared spectrum sees image 3 .

Embodiment 3

[0035] 1) Add 4g of pectin powder to a 100ml Erlenmeyer flask, then add distilled water to 100ml, shake to completely dissolve the pectin.

[0036] 2) When the pectin is completely dissolved, add 6ml of 30g / L ammonium acetate solution, and stir at 50°C for 12h.

[0037] 3) Use hydrochloric acid to adjust the pH of the solution to 3, then add 5 times the volume of absolute ethanol, stir evenly, filter to obtain pectin precipitate, dry with hot air at 60°C to constant weight, and pulverize to obtain low-ester pectin.

[0038] The esterification degree of gained low-ester pectin is 45%, and infrared spectrum sees Figure 4 .

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Abstract

The invention discloses a method using an ammonium exchange method to prepare low-methoxyl pectin. The method is characterized in that ammonium acetate is added into a high-methoxyl pectin solution and the reaction condition is strictly controlled to prepare the low-methoxyl pectin. Compared with a traditional alkaline hydrolysis method, the method has the advantages that the reaction is performed under a neutral condition, the beta elimination reaction is avoided, and pectin molecular weight is unaffected; in addition, the method is mild in reaction condition, simple in process flow, low in production cost, and the like.

Description

technical field [0001] The invention belongs to the technical field of food processing, and in particular relates to a method for preparing low-ester pectin by an ammonium group exchange method. Background technique [0002] As a common food additive, pectin is widely used in food and pharmaceutical industries. Acid hydrolysis is the most commonly used method to extract pectin. Pectin methyl esterification is one of the important indicators affecting the properties of pectin. According to the degree of methyl esterification, pectin is divided into high-ester pectin (esterification degree> 50%) and low-ester pectin (degree of esterification <50%), studies have shown that most of the pectin extracted by acid hydrolysis is high-ester pectin. [0003] As a polymer polysaccharide compound, pectin has attracted much attention for its unique physical and chemical properties. Studies have found that low-ester pectin has anti-cancer functions. The reported methods for prepari...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C08B37/06
CPCC08B37/0003C08B37/0045
Inventor 潘思轶范传会胡海娟王鲁峰王可兴
Owner HUAZHONG AGRI UNIV
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