Construction method of bacterial septicemia infected grass carp disease model

Abstract

The invention relates to a construction method of a bacterial septicemia infected grass carp disease model. The method comprises the following steps: 1, selecting grass carps with the weight of 45-55g, dividing the selected grass carps into a control group and an injection group, injecting 1*10<7>cfu / ml of Aeromonas hydrophila to the injection group grass carps, and injecting equivalent normal saline to the control group grass carps; 2, respectively breeding the two groups of grass carps, respectively taking bloods on the same day, first day, third day, seventh day, fourteenth day and twenty-first day, and analyzing the taken bloods; and 3, carrying out following detections: 1) erythrocyte counting, 2) leucocyte counting, 3) hematocrit value determination, 4) mean corpuscular volume determination, 5) white blood cell differential count, 6) total protein determination, 7) lysozyme determination, 8) peroxidase determination, 9) immunoglobulin M determination, and 10) total complement determination. The construction method of the bacterial septicemia infected grass carp disease model provides scientific research guidance for fish immunology researches, discloses the fish disease degree and the administration dosage in breeding production, and lays a certain foundation for scientific and healthy development of the aquatic product breeding industry.

Description

technical field [0001] The invention relates to the technical field of aquaculture, in particular to a method for constructing a grass carp disease model infected with bacterial sepsis. Background technique [0002] Grass carp is a herbivorous fish, and it is also the main target of my country's excellent traditional freshwater fishery and freshwater aquaculture. Grass carp aquaculture has a history of more than 1,700 years in my country. In 2012, the output of grass carp in my country was more than 4.44 million tons, accounting for 20% of the total output of freshwater aquaculture fish in my country (Fishery Statistical Yearbook, 2012), ranking first in the output of freshwater aquaculture fish in the world. Bacterial sepsis is also known as bacterial hemorrhagic disease and explosive hemorrhagic disease. It was first discovered in the late 1970s and early 1980s. It is an acute transfection disease caused by pathogenic Aeromonas hydrophila. It can be infected from fingerli...

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And about the infection bacterial sepsis grass carp disease model of the present invention, there is no report yet

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