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Seven-link gold label detection card and preparation method thereof

A gold label and detection box technology, applied in the medical field, can solve the problems of immature technology, time-consuming and expensive

Inactive Publication Date: 2016-08-31
FUZHOU GENERAL HOSPITAL OF NANJING MILITARY COMMAND P L A +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Indirect immunofluorescence requires skilled technicians to operate, and it is time-consuming and cannot be processed in batches. Chemiluminescence requires a special analyzer, which is expensive and the technology is not yet mature. At the same time, the colloidal gold method is simple to operate, convenient to use, and fast in detection speed. At the same time, due to the partial overlap of various clinical symptoms, each individual diagnosis is expensive and time-consuming, and it is difficult to provide doctors with a comprehensive judgment basis.

Method used

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  • Seven-link gold label detection card and preparation method thereof
  • Seven-link gold label detection card and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] Please refer to figure 1 and figure 2, a seven-joint gold standard detection box, the detection box includes an upper casing and a lower casing, and the detection box includes 4 detection units (1, 2, 3 , 4), the upper housing is provided with 4 sample holes 7 for adding samples and observation windows for observing the test results, which are used in conjunction with the detection units (1, 2, 3, 4) 11. The detection unit (1, 2, 3, 4) includes an antigen-coated nitrocellulose membrane 10 arranged on the isolation tank 5 on the lower casing and the bottom layer of the isolation tank 5, and is arranged on the nitrocellulose The coated gold standard pad 8 at the front end of the upper layer of the plain film 10, the liquid absorption pad 9 located at the upper rear end of the nitrocellulose membrane 10, the sample pad 7 that is located at the corresponding position of the sample hole on the coated gold standard pad 8, and A control band C and one or more detection band...

Embodiment 2

[0035] The preparation method of the seven-link gold standard detection card is as follows:

[0036] 1. Coated nitrocellulose membrane

[0037] 1) Prepare coating solution: prepare SmD1, SmB', SSA52, SSA60, SSB, U1RNP and Jo-1 antigens, and goat anti-mouse IgG quality control to the corresponding coating concentrations, respectively 0.5mg / ml and 1 mg / ml, 1.5 mg / ml, 2 mg / ml, 2.5 mg / ml, 3 mg / ml, 3.5 mg / ml, 4 mg / ml, 4.5 mg / ml, 5mg / ml;

[0038] 2) Coating antigen: According to the size of the immunoblotting spotter, cut the nitrocellulose membrane into a membrane piece, and wrap the spotting solution prepared in 1) in a certain order by physical adsorption and covalent bonding. On the membrane, one sample band is coated with SmD1 and SmB' antigens expressed by yeast recombinant expression, one sample band is coated with SSA52KD, SSA60KD, and SSB antigens expressed by yeast recombinant expression, and the sample band is coated with yeast recombinant expressed A sample band expre...

Embodiment 3

[0051] When testing, place the test card flat on the laboratory table, add 100 μl of the sample to be tested into the four sample holes with a dropper or a sample gun, and judge the result from the observation window after 15-20 minutes. If the control bands are all colored, it means that the sample contains specific antibodies to the antigen coated by the test band. If the test band does not develop color and only the control band develops color, it means that the sample does not contain the specificity of the antigen coated by the test band. If the antibody or concentration does not reach a detectable level, if neither the test band nor the control band develops color, it indicates that the test is invalid. In actual operation, the test card works very well. Compared with a single test, it saves a lot of time and cost, and can give doctors a comprehensive evaluation reference.

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Abstract

The invention relates to a seven-link gold label detection card, relates to the technical field of medical science and specifically relates to a novel autoantibody seven-link gold label detection card based on a yeast recombinant expression autoantigen. In order to quickly supply a synthetic judgment basis to a doctor, the invention provides a gold label detection kit. The detection kit comprises an upper shell and a lower shell, wherein a detection unit is arranged in the lower shell; and an observation window is arranged on the upper shell; the detection unit comprises a nitrocellulose membrane, a coated gold label pad, a liquid absorbing pad, a sample pad and a detection belt, wherein the coated gold label pad is arranged at the front end of the upper layer of the nitrocellulose membrane. The seven-link gold label detection card has the beneficial effects that the diagnostic sensibility for systemic lupus erythematosus, dry syndrome, mixed connective tissue diseases and myositis / dermatomyositis can be increased by jointly detecting seven autoantibodies in serum or plasma, the comprehensive analysis for the illness state stage is more benefited, and the gold label detection kit has important significance in judging the type of illness state, tracking and prognosis.

Description

technical field [0001] The invention relates to the field of medical technology, in particular to a novel autoantibody seven-link gold label detection card based on yeast recombined expression of autoantigens. Background technique [0002] Autoimmune disease is the third most common disease after cardiovascular disease and cancer, its overall incidence accounts for 3% to 5% of the world's population, and it is still on the rise, and the range of autoimmune diseases is wide , including SLE (systemic lupus erythematosus), SS (Sjögren's syndrome), mixed connective tissue disease (MCTD), polymyositis / dermatomyositis antibody (PM / DM), etc., there are some cross-talks in various clinical symptoms. It is difficult to establish clear judgment criteria. [0003] Anti-Sm antibodies have high specificity for the diagnosis of SLE, and are currently recognized serum marker antibodies for SLE. SmD1 and SmB' polypeptides are the most common anti-Sm specific autoimmune response targets, wh...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/564G01N33/558
CPCG01N33/564G01N33/558
Inventor 杨湘越王开宇冯福英廖剑赵猛陈敏兰小鹏钱纯亘王栋凯
Owner FUZHOU GENERAL HOSPITAL OF NANJING MILITARY COMMAND P L A
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