Tissue culture method of phalaenopsis

A technique for tissue culture and phalaenopsis, applied in the field of tissue culture of Phalaenopsis, can solve the problems of slow germination and low number of seedlings, and achieve the effects of increasing germination, increasing the number of seedlings, and promoting leaf growth.

Inactive Publication Date: 2016-09-21
HEFEI LONGYANG AGRI TECH CO LTD
View PDF5 Cites 2 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The current tissue culture method of Phalaenopsis has the problems of slow germination and low number of seedlings

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] A method for tissue culture of Phalaenopsis, comprising the steps of:

[0026] (1) Seed collection

[0027] First select the Phalaenopsis fruit pods 95 days after pollination, the selected fruit pods are smooth and full outside, and the color turns from green to yellow;

[0028] (2) Inoculation

[0029] First scrub the surface of the capsule with a diluted detergent solution, then rinse it, then soak it in 8% NaClO for 10 minutes, then disinfect it with 75% alcohol for 1 minute on the ultra-clean workbench, and disinfect it with 0.05% mercury liter for 3 minutes, then wash it with sterile water Rinse twice, take out the capsules and put them in a sterile petri dish, cut the capsules and move the seeds into a culture bottle with sterile water, shake gently to disperse them evenly, and the inoculation density is 200 grains / m 2 ;

[0030] (3) germination

[0031] Seeds are germinated using seed sterile germination medium. After 10 days of inoculation, the embryos germi...

Embodiment 2

[0039] A method for tissue culture of Phalaenopsis, comprising the steps of:

[0040] (1) Seed collection

[0041] First select the Phalaenopsis pods 100 days after pollination, the selected pods are smooth and full outside, and the color turns from green to yellow;

[0042] (2) Inoculation

[0043] First scrub the surface of the capsule with a diluted detergent solution, then rinse it, then soak it in 10% NaClO for 15 minutes, then disinfect it with 75% alcohol for 2 minutes on the ultra-clean workbench, and disinfect it with 0.10% mercury liter for 3 minutes. Rinse 3 times, take out the capsules and put them in a sterile petri dish, cut the capsules and move the seeds into a culture bottle with sterile water, shake gently to disperse them evenly, and the inoculation density is 250 grains / m 2 ;

[0044] (3) germination

[0045] Seeds are germinated using seed sterile germination medium. After 15 days of inoculation, the embryos germinate, and after 20 days, they are trans...

Embodiment 3

[0053] A method for tissue culture of Phalaenopsis, comprising the steps of:

[0054] (1) Seed collection

[0055] First select the Phalaenopsis pods 105 days after pollination, the selected pods are smooth and full outside, and the color turns from green to yellow;

[0056] (2) Inoculation

[0057] First scrub the surface of the capsule with a diluted detergent solution, then rinse it, then soak it in 12% NaClO for 20 minutes, then disinfect it with 75% alcohol for 3 minutes on the ultra-clean workbench, and disinfect it with 0.15% mercury liter for 3 minutes. Rinse 4 times, take out the capsule and put it in a sterile petri dish, cut the capsule and move the seeds into a culture bottle with sterile water, shake gently to disperse them evenly, and the inoculation density is 300 grains / m 2 ;

[0058] (3) germination

[0059]Seeds are germinated using seed sterile germination medium. After 1020 days of inoculation, the embryos germinate, and after 20 days, they are transfer...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention discloses a tissue culture method of phalaenopsis. In the steps, the inoculation density and seeding density can generate a 'group effect' so as to accelerate seed germination; a sterile germination culture medium consists of tryptone, banana juice, active carbon and agar in a ratio and can provide nutrition for the seed growth and promote the seed germination; hormone is added at the time of germination, and the number of live seedlings can be increased; an organic additive is added during germination, wherein the organic additive is 24% sucrose, and a promotion effect can be realized on the phalaenopsis seed germination, protocorm formation and blade growth. By adopting the tissue culture method, the cultured phalaenopsis has the advantages of high germination rate and sound growth and also has huge market potential and broad prospect.

Description

technical field [0001] The invention belongs to the field of flower cultivation, and more specifically, the invention relates to a tissue culture method of Phalaenopsis. Background technique [0002] Phalaenopsis, orchidaceae Phalaenopsis, is a single-stem tropical epiphytic orchid, distributed in tropical and subtropical regions of Asia and Oceania, and mostly grows on tree trunks and wet stones in damp, foggy and high-temperature forests. Showy, with a long flowering period, it has become one of the most popular flowers in the international and domestic flower markets. It is known as the "Queen of Orchids". Through sterile seeding and tissue culture, a large number of excellent plants can be obtained, which can maintain excellent quality characteristics, and is the key to large-scale commercial production and new variety breeding. The current tissue culture method of Phalaenopsis has the problems of slow germination and low seedling quantity. Contents of the invention ...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
CPCA01H4/008A01H4/001
Inventor 岳朝雷
Owner HEFEI LONGYANG AGRI TECH CO LTD
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap