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An artificial culture method for ghost moth larvae, the hosts of ophiocordyceps sinensis

A technology of bat moth larvae and Cordyceps sinensis fungus, applied in animal husbandry and other fields, can solve problems such as death, weight gain, and decrease in the number of insect populations, and achieve a high survival rate effect

Inactive Publication Date: 2016-09-21
ZHEJIANG FORESTRY UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0008] The weight of host bat moth larvae increases significantly in the later stage of artificial feeding, the scope of activities expands, feeding and excretion increase significantly, the living environment deteriorates, and frequent pests and diseases, especially diseases caused by pathogenic bacteria and fungi, often lead to large-scale infection of larvae , death, and the number of worms dropped sharply, resulting in a very low survival rate of larvae in the late stage of artificial feeding
In low-altitude areas, there is still no effective way to raise bat moth larvae artificially for a long time to reduce diseases and increase the survival rate of larvae

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] A method for artificially cultivating Cordyceps sinensis host bat moth larvae, comprising the following steps:

[0029] A, gather chrysalis, gather the chrysalis of the wild bat moth in alpine meadow zone, preserve,

[0030] B. Collect the feeding substrate, collect the plateau humus around the area where an appropriate amount of chrysalis is excavated,

[0031] C. Eclosion and mating, place the pupae in a container filled with plateau humus, and the pupae are located 2-3cm below the soil, and use a gauze around the container, eclosion at 9-12°C, and eclosion into a host After the bat moth, mating and laying eggs are completed in 2-5 days, and the eggs of the host bat moth are immediately stored in an environment with a relative humidity of 80% after laying eggs.

[0032] D, hatching, get worm's ovum and place on humus surface layer, put into constant temperature incubator and carry out dark hatching, constant temperature incubator temperature is 15 ℃, relative humidit...

Embodiment 2

[0035] This embodiment is basically the same as Embodiment 1, except that, in step B, wild Polygonum viburnum near the pupae is also excavated when collecting pupae.

[0036] In step E, the feeding substrate is uniformly mixed with sand: sawdust: plateau humus at a weight ratio of 1:2:2, and sterile water is added while mixing to adjust the moisture content of the medium to 55%-65%. . Add the solid female species of Flammulina velutipes and stir well. Control the relative humidity of the culture room at about 70%, control the temperature at about 20-23°C, and cultivate for 10-14 days. Before mixing, the plateau humus soil was baked at 60°C for more than 4 hours, the clean water sand was baked at 180°C for more than 2 hours, and the sawdust was sterilized by high-pressure steam at 121°C for more than 30 minutes.

Embodiment 3

[0038] This embodiment is basically the same as Example 1, the difference is that in step E, the feeding substrate is a mixture of clear water sand, sawdust and plateau humus with a weight ratio of 5:2:6, and the feeding substrate The water content is 55-65%. Before mixing, the plateau humus soil was baked at 60°C for more than 4 hours, the clean water sand was baked at 180°C for more than 2 hours, and the sawdust was sterilized by high-pressure steam at 121°C for more than 30 minutes.

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PUM

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Abstract

The invention belongs to the technical field of insect culture and relates to an artificial culture method for ghost moth larvae, the hosts of ophiocordyceps sinensis. The method comprises the steps of A, collecting pupae of wild ghost moths in an alpine meadow region; B, collecting a proper amount of plateau humus on the periphery of a pupa digging area; C, placing the pupae in a container containing the plateau humus at a depth of 2-3cm in the humus, surrounding the container with a gauze element, wherein eclosion occurs at a temperature of 9-12 DEG C and host ghost moths from the eclosion complete mating and egg laying in 2-5d; D, placing the eggs at the surface layer of the humus, placing the eggs in a constant temperature culture tank for light-avoiding hatching to hatch the eggs into larvae, the temperature of the constant temperature culture tank being 15 DEG C and the relative humidity being 80-90%; E, preparing a breeding box, placing a feeding medium in the breeding box, transferring the larvae in the breeding box, placing feedstuff in the box and breeding the larvae at a temperature of 10-15 DEG C. The artificial culture method for ghost moth larvae, the hosts of ophiocordyceps sinensis has the advantage of high survival rate.

Description

technical field [0001] The invention belongs to the technical field of artificial cultivation of insects, and relates to a method for artificial cultivation of bat moth larvae, the host of Cordyceps sinensis fungus. Background technique [0002] Cordyceps sinensis is a unique and precious specialty in my country, also known as Cordyceps sinensis. Because of its powerful health care effects, it is as famous as deer antler and ginseng, and is listed among the three major supplements. At the beginning of the research, some people once classified Cordyceps sinensis into the order Hypocreeales, but according to the recent systematic classification, Cordyceps sinensis should belong to Fungi, Ascomycota, Ascomycetes, and Faecalibacterium. Class Sordariomycetidae, Hypocreales, Clavicipifaceae, Cordyceps. Therefore, Cordyceps sinensis is a compound of the sub-seat and the larva corpse of the fungus Cordyceps sinensis of the family Ergotaceae parasitizing on the overwintering larvae...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01K67/033
CPCA01K67/033
Inventor 林海萍朱旭伟郭恺周湘苏秀李朋举
Owner ZHEJIANG FORESTRY UNIVERSITY
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