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Method for determination of curculigine in curculigo orchioides

A technology for curculin and curcuma, applied in the field of laboratory testing, can solve problems such as unpublished extraction and determination methods of curculin, and achieve the effects of high extraction efficiency and accurate determination

Inactive Publication Date: 2016-09-21
WUZHOU INST FOR FOOD & DRUG CONTROL
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Pharmacopoeia only records the extraction and determination method of curculin, and there is no relevant literature in the prior art to disclose the extraction and determination method of curculin

Method used

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  • Method for determination of curculigine in curculigo orchioides
  • Method for determination of curculigine in curculigo orchioides

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Embodiment 1

[0024] The curculigo sample was pulverized by a pulverizer, passed through a No. 3 sieve, about 1g, weighed accurately, mixed with 0.5g of diatomaceous earth, and transferred to the pre-placed filter membrane. Add an appropriate amount of diatomaceous earth to the 10ml extraction pool, shake gently to make it on the same level as the pool mouth, and tighten the upper cover of the extraction pool. After the extraction, transfer the extract to a 50ml volumetric flask, dilute to the mark with methanol, centrifuge at 15000r / min for 5min, take the supernatant, and enter it into LC for determination.

[0025] The extraction conditions (see table 1) are:

[0026] Table 1

[0027]

[0028]

[0029] Analysis method is LC liquid chromatography, liquid chromatography analysis conditions:

[0030] a) Instrument: Thermo double ternary liquid phase (U-3000)

[0031] b) Chromatographic column: Thermo Syncronis C18 3*100mm 3um, that is, carbon 18 chromatograph, diameter 3mm, length ...

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Abstract

The invention discloses a method for determination of curculigine in curculigo orchioides, wherein the method comprises the following steps: step 1, crushing the curculigo orchioides, then sieving, mixing 1 part by weight of the curculigo orchioides powder obtained from crushing and 0.5 part by weight of diatomite, adding the mixture to an extraction pool, carrying out static extraction, and extracting by using a rapid solvent extraction method to obtain an extract liquid, wherein extraction conditions comprise that an extraction solvent is a mixed solution of isopropanol and methanol with the weight ratio of 2:8, the extraction temperature is 100 DEG C and the static extraction time is 5 min, and the flushing volume is 100%; and carrying out static circulation for 1 time; step 2, carrying out dilution, centrifugation and filtration of the extract liquid, to obtain a supernatant; and step 3, analyzing the supernatant by liquid chromatography. The invention provides the method for determination of curculigine in the curculigo orchioides; the method has the advantages of simple operation and high detection accuracy.

Description

technical field [0001] The invention relates to the field of laboratory detection, in particular to a method for determining curculin in curculigo. Background technique [0002] The 2015 Pharmacopoeia recorded the extraction and determination method of curculigoside in Curculigo curculifolia: Take about 1g of the powder of this product (passed through a No. 3 sieve), accurately weigh it, accurately add 50ml of methanol, weigh it, heat and reflux for 2 hours, take it out, and let it cool , and then weighed, make up the lost weight with methanol, shake well, and filter. Precisely measure 20ml of the follow-up filtrate, evaporate to dryness, add methanol to dissolve the residue, transfer to a 10ml measuring bottle, add methanol to the mark, shake well, filter, take the follow-up filtrate, and obtain. [0003] The detection conditions of liquid chromatography are as follows: octadecylsilane bonded silica gel is used as filler; acetonitrile-0.1% phosphoric acid solution (21:79) ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/02G01N30/06
CPCG01N30/02G01N30/06G01N2030/027G01N2030/062
Inventor 陈学松王丽丽韦涛梁美艳黄新惠
Owner WUZHOU INST FOR FOOD & DRUG CONTROL