Planting method for saffron crocus tissue culture micro bulbotubers

A technology of tissue culture and planting methods, applied in botany equipment and methods, cultivation, plant cultivation, etc., can solve the problems of limited resources, high price, bulb degeneration, etc., and achieve high planting survival rate, less virus content, and simple operation Effect

Inactive Publication Date: 2016-09-28
江苏丰收大地种业发展有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, only the stigma of saffron can be used as medicine. Due to factors such as limited areas suitable for cultivation and harsh conditions, the yield is extremely low and resources are extremely limited, resulting in high prices. It has always been known as "plant gold".
Because saffron cannot reproduce sexually, it can only reproduce asexually through bulbs, and its bulbs degenerate seriously under cultivation conditions, resulting in a serious shortage of germplasm resources.
Since the introduction of the species in my country, the yield of cultivated saffron has always been low, which is far from meeting people's needs.
In recent years, tissue culture technology has been applied to the production of saffron seedlings, which provides a solution to the shortage and degradation of saffron bulbs. However, plant tissue culture is a labor-intensive industry, and the production cost remains high. The production of seedlings is also difficult. only in the experimental stage

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0017] Embodiment 1: the implantation method of this saffron tissue culture microbulb, comprises the following steps:

[0018] (1) Provenance preparation: including two steps of seed selection and sterilization;

[0019] Seed selection: choose plant solid tissue culture obtained saffron micro-bulbs as the planting provenance, and the weight of the saffron micro-bulbs is 6 ~ 8g;

[0020] Sterilization: Wash the above-mentioned microbulbs with clean water, remove the attached agar, and then immerse them in 0.1% potassium permanganate sterilizing solution for 20 minutes, then wash off the residual sterilizing solution on the surface, and spread them out in a dark and ventilated place Drying; wherein, in the sterilization step, stirring can be done during the process of soaking the microbulbs, but sticks cannot be used to stir;

[0021] (2) Soil preparation: including four steps of planting the field, applying basal fertilizer, soil disinfection, and cultivating ridges.

[0022]...

Embodiment 2

[0029] Embodiment 2: the planting method of this saffron tissue culture microbulb, comprises the following steps:

[0030] (1) Provenance preparation: including two steps of seed selection and sterilization;

[0031] Seed selection: the saffron microbulbs obtained by plant intermittent immersion liquid culture are used as planting provenance, and the weight of the microbulbs is 5 ~ 7g;

[0032] Sterilization: Wash the above-mentioned microbulbs with clean water, then immerse them in 0.1% potassium permanganate sterilizing solution for 25 minutes, then wash off the residual sterilizing solution on the surface, spread them out in a dark and ventilated place to dry; among them, the sterilization step In the process of soaking the microbulbs, you can stir, but you can't use a stick to stir;

[0033] (2) Soil preparation: including four steps of planting the field, applying basal fertilizer, soil disinfection, and cultivating ridges.

[0034] Planting field: use the flat land nea...

Embodiment 3

[0041]Embodiment 3: the implantation method of this saffron tissue culture microbulb, comprises the following steps:

[0042] (1) Provenance preparation: including two steps of seed selection and sterilization;

[0043] Seed selection: the Chinese patent application number is: 201510263701.9 "A method for producing saffron microbulbs by shallow cultivation" obtained as planting provenance, and the cultivation methods of the saffron microbulbs are all introduced here; Said microbulb weight is 7~10g;

[0044] Sterilization: wash the above-mentioned microbulbs with clean water, and then immerse them in a 0.1% potassium permanganate sterilizing solution for 30 minutes, then wash off the residual sterilizing solution on the surface, and spread them out in a dark and ventilated place; wherein, in the sterilization step Stirring is allowed during soaking of the microbulbs, but not with a stick;

[0045] (2) Soil preparation: including four steps of planting the field, applying basa...

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PUM

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Abstract

The invention discloses a planting method for saffron crocus tissue culture micro bulbotubers, and technical service is provided for seedling hardening planting in the later period of saffron crocus plant tissue culture. The planting method for saffron crocus tissue culture micro bulbotubers includes the following steps of seed source preparing, wherein seed selecting and sterilizing are involved; soil preparation, wherein the substeps of planting in a field, applying base fertilizer, disinfecting soil and carrying out furrow opening and ridging are included; planting and observing, wherein two substeps of sowing and observing are included. As the bulbotubers and soil are sterilized and disinfected by adopting multiple disinfecting methods, damage to the tissue culture micro bulbotubers by microorganisms is eliminated or reduced, and the planting survival rate is large; the method of two-time deep ploughing is adopted, and the soil is loosened, which is more beneficial for growth of saffron crocus. The planting method is easy to operate and low in cost, and large-scale industrial production is easier to achieve.

Description

technical field [0001] The invention relates to a method for cultivating Chinese herbal medicines, in particular to a method for planting saffron tissue cultured microbulbs. Background technique [0002] Saffron (Crocus sativus L.), also known as saffron and saffron, is a perennial flower of the genus Crocus in the family Iridaceae, and is also a common spice. It is a native species in Southwest Asia and was first cultivated by the Greeks. It is mainly distributed in Europe, the Mediterranean, and Central Asia. It was introduced into China during the Ming Dynasty, and it was listed as a medicine in the "Compendium of Materia Medica". It is currently cultivated in Jiangsu, Zhejiang, and North China. It is a valuable traditional Chinese medicine. material. Spices and main natural pigments such as saffron, saffron picroside and saffron aldehyde can be extracted from its stigma. Saffron is an active ingredient with significant curative effect on coronary heart disease. Small,...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01G1/00
CPCA01G22/00
Inventor 陈明亮周志疆张丽丽秦路平
Owner 江苏丰收大地种业发展有限公司
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