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Production method of colorful bone sample

A specimen and bone technology, applied in the field of biological specimens, can solve the problems of difficult to achieve flexible and complete bone specimens, insufficient fixation, ambiguous concentration, etc., and achieve the effects of colorful, easy operation, and short production cycle.

Active Publication Date: 2016-09-28
马征来
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The fixation in this patent is not sufficient, and when staining, the specificity is poor, the concentration of glacial acetic acid is relatively fixed, it is easy to remove the calcium in the calcified tissue, the concentration of potassium hydroxide is relatively vague, and it is difficult to achieve flexible and complete bone specimens

Method used

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  • Production method of colorful bone sample

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0039] A method for preparing a colorful bone specimen, comprising the steps of:

[0040] S1. Material collection: Peel and remove the viscera from 12-day-old chicken embryos to obtain fish specimens;

[0041] S2. Fixation: the animal specimens obtained in step S1 were fixed by soaking in 80 volume % ethanol for 12 hours, 50 volume % methanol for 6 hours, 50 volume % acetone for 6 hours, and 60 volume % ethanol for 12 hours; the fixative used was 80 The volume ratio between volume % ethanol, 50 volume % methanol, 50 volume % acetone and 60 volume % ethanol and animal specimen is 20:1;

[0042] S3. Refixation: Soak the animal specimen obtained in step S2 with 60% by volume alcohol, and fix it at 0°C for 1 day;

[0043]S4. Staining: Skeletal staining of the fixed animal specimens in step S3 with Alcian blue staining solution;

[0044] S5. Staining with Alizerin red staining solution. After staining with Alizerin red staining solution, wash the floating color with 0.5-3% potass...

Embodiment 2

[0055] A method for preparing a colorful bone specimen, comprising the steps of:

[0056] S1. Material collection: remove the skin and viscera of the fish to obtain fish specimens;

[0057] S2. Fixation: the animal specimens obtained in step S1 were fixed by soaking in 90 volume % ethanol for 8 hours, 65 volume % methanol for 9 hours, 65 volume % acetone for 9 hours and 75 volume % ethanol for 18 hours; the fixative used was 90 The volume ratio between volume % ethanol, 65 volume % methanol, 65 volume % acetone and 75 volume % ethanol and animal specimen is 25:1;

[0058] S3. Refixation: Soak the animal specimen obtained in step S2 with 75% by volume alcohol, and fix it for 2 days at a temperature of 2°C;

[0059] S4, dyeing: adopting Alizerin red staining solution to carry out bone staining processing to the animal specimen fixed in step S3;

[0060] S5. Transparency: Soak in 65% glycerol for 9 hours, 90% glycerol Ⅰ for 3 days, and 95% glycerol Ⅱ for 9 days in sequence to m...

Embodiment 3

[0068] A method for preparing a colorful bone specimen, comprising the steps of:

[0069] S1. Material collection: peel and viscera the Brazilian tortoise to obtain specimens of the Brazilian tortoise;

[0070] S2. Fixation: the animal specimens obtained in step S1 were fixed by soaking in 100% ethanol for 24 hours, 75% methanol for 12 hours, 75% acetone for 12 hours, and 95% ethanol for 24 hours in sequence; the fixative used was 100 The volume ratio between volume % ethanol, 75 volume % methanol, 75 volume % acetone and 95 volume % ethanol and animal specimen is 30:1;

[0071] S3. Refixation: Soak the animal specimen obtained in step S2 with 95% by volume alcohol, and fix it at 4°C for 3 days;

[0072] S4, dyeing: adopting Alizerin red staining solution to carry out bone staining processing to the animal specimen fixed in step S3;

[0073] S5. Transparency: Soak in 70% by volume glycerol for 12 hours, soak in 100% by volume glycerol I for 5 days, and soak in 100% by volume...

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Abstract

The invention discloses a production method of a colorful bone sample. The method comprises the steps of drawing a material, fixing the material, dyeing the fixed material, vitrifying the dyed material, and carrying out microscopic treatment, wherein a re-fixing step is arranged after the fixing step and before the dyeing step, and the re-fixing step is characterized in that an obtained animal sample is immersed and fixed in 60-95 V% alcohol at 0-4DEG C for 1-3d. The colorful bone sample produced in the invention has the advantages of beautiful profile, high transparence, high fidelity, short production period and realization of batch production.

Description

technical field [0001] The invention relates to the field of biological specimens, in particular to a method for preparing colorful bone specimens. Background technique [0002] At present, most of the animal specimens produced in the domestic market are preserved by applying preservatives and then air-dried, or directly fixed and preserved in formalin. . There are researches on bone art in foreign countries, forming an art trend. At present, the research on bone art in China is in the initial stage, the production technology is relatively rough, and there is also a lack of large-scale manufacturers of bone specimens. [0003] With the deepening of research, the Chinese patent "Method for making crystal bone specimens" with application number 201510284053.5 discloses a method for making crystal bone specimens. The method includes the following steps: after obtaining animal specimens, use 50% ethanol, 100% Methanol, 100% acetone and 100% ethanol to fix animal specimens; the...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N1/28G01N1/30
CPCG01N1/28G01N1/30
Inventor 陈彬彬陈华彬
Owner 马征来
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