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A kind of production method of arginine active peptide powder for livestock and poultry

A production method and technology of arginine, applied in the field of arginine fermentation technology and microbial solid fermentation, can solve the problems of lack of brand, lack of protein, lack of quality and safety brands, etc.

Active Publication Date: 2021-03-12
战晓燕 +2
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] At present, the problems faced by my country's animal husbandry and feed production industry are: lack of protein, lack of high-quality protein, lack of brand, lack of quality and safety brand, which is the direction we are working hard for
But generally speaking, the application of domestic arginine feed additive is in its infancy. Academician Yin Yulong’s research shows: 1. Piglets are supplemented with 0.4% arginine on 21-28 days, and 0.2% arginine on 28-35 days , the individual differences of piglets are reduced, and the average daily gain is increased

Method used

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  • A kind of production method of arginine active peptide powder for livestock and poultry
  • A kind of production method of arginine active peptide powder for livestock and poultry
  • A kind of production method of arginine active peptide powder for livestock and poultry

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Experimental program
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Effect test

preparation example Construction

[0043] (1) Preparation of arginine concentrated bacterial liquid wet powder: Inoculate the arginine-producing bacteria strain into the arginine-producing bacteria fermenter seed medium, and the arginine-producing bacteria fermenter seed medium is expressed in weight percentage It is: 2.0% of glucose, 2.0% of glutamic acid, 3.0% of corn steep liquor dry powder, 0.1% of ammonium sulfate, 0.1% of magnesium sulfate, 0.1% of dipotassium hydrogen phosphate, the balance is water, and the inoculum size is 10%. After 48 hours of cultivation, the number of viable bacteria containing arginine-producing bacteria is obtained ≥ 10 billion / ml seed liquid.

[0044] Concentration process and reaction conditions of arginine concentrated bacterial liquid wet powder. First, put 4 tons of arginine fermentation liquid into a 5-ton reaction kettle, start stirring, add flocculant at 0.1% to 0.4%, and use sulfuric acid to Adjust the pH value of the arginine fermentation liquid to 5.0-6.5, react at a t...

Embodiment 1

[0108] Concentration process and confirmation of reaction conditions of arginine concentrated bacterial liquid wet powder

[0109] Pour 4 tons of arginine fermentation broth into a 5-ton reactor, start stirring, add flocculants (both produced by Aisen China Flocculant Co., Ltd.) at 0.1% to 0.4%, and ferment the arginine with sulfuric acid Adjust the pH value of the liquid to 5.0-6.5, react at a temperature of 40°C-55°C, and the reaction time is 50-80 minutes, and then press it into the filter press with a pressure of 3mpa, and the filter press time is 20-50 minutes. The arginine concentrated bacterial liquid wet powder with a water content of 20% to 30% is obtained.

[0110] Concentration process and condition test of arginine concentrated bacterial liquid wet powder

[0111] The experimental design is 5 factors and 4 levels. The experimental design and experimental results are shown in Table 1:

[0112] Table 1. Concentration and reaction condition test of arginine concentr...

Embodiment 2

[0116] Formulation of Arginine-producing Bacteria Fermenter Seed Medium Formula and Confirmation of Fermentation Conditions

[0117] The arginine-producing bacteria are Brevibacterium flavums. The main features are: 1. Using glutamic acid as the fermentation substrate, the nutritional requirements are high carbon and high nitrogen, and can successfully metabolize L-arginine 2. Nitrogen and carbon are added during the fermentation process to synthesize arginine and peptide substances, so as to obtain a fermentation broth with a higher arginine content. Arginine-producing bacteria also have the characteristics of fast reproduction speed, good stability, and wide application range.

[0118] The specific operation steps of single colony purification and optimization, solid eggplant-shaped flat bottle seed cultivation, and fermenter submerged fermentation seed cultivation are as follows:

[0119] Single colony purification: see (7) 1) of raw material preparation step;

[0120] So...

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Abstract

The invention relates to a production method of arginine active peptide powder for livestock and poultry. The wet powder of arginine thickened bacterial liquid, soybean meal, and dry powder of corn steep liquor are used as raw materials, and arginine-producing bacteria, Bacillus subtilis, Saccharomyces cerevisiae, and Lactobacillus plantarum are used as beneficial microbial flora, and four kinds of microbial liquid seeds are respectively used Solid eggplant-shaped flat bottle seed culture and fermenter seed culture. After the solid and the liquid are evenly mixed in proportion, they are placed on a fermentation bed, and sterile air is introduced to carry out aerobic solid fermentation, and then dried, crushed, and sieved to obtain arginine active peptide powder for livestock and poultry. The invention has the characteristics of wide sources of fermentation raw materials, mature fermentation process, high protein content, high arginine content, high conversion rate of small peptides, good application effect and the like. This product is used in pigs, chickens, cattle and other livestock and poultry breeding. It can coordinate amino acid metabolism and promote protein synthesis, and can improve animal immunity, disease prevention and resistance, reproductive ability, production performance and growth speed.

Description

technical field [0001] The invention relates to arginine fermentation technology and microbial solid fermentation technology, in particular to a production method of arginine active peptide powder for livestock and poultry. Background technique [0002] my country is the world's largest animal husbandry country, as well as the world's largest feed producer and consumer. According to statistics, the total production of feed in 2015 was 220 million tons, accounting for more than half of the global total. Among them, 25 million tons of concentrated feed, additives And 6.5 million tons of premix. [0003] In recent years, people's dietary structure and consumption concepts have undergone tremendous changes with the improvement of living standards. People have changed from eating full, good, and refined to eating safe and healthy. The animal husbandry industry and feed production industry are changing from quantity to quality. Continuously reform and innovate development ideas a...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20C12N1/14A23K10/12A23K20/142A23K20/147
CPCA23V2400/169
Inventor 战晓燕刘志国
Owner 战晓燕
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