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A kind of aroma-producing fungus and its application

A technology of fungi and strains, applied in the field of microorganisms, can solve the problems of tomato production and post-harvest preservation, fruit rot, hazards during transportation and storage, etc., and achieve good biocontrol effects and broaden research ideas.

Active Publication Date: 2019-10-22
XINYANG AGRI & FORESTRY UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In addition to causing fruit rot during the growth period, it can also continue to cause harm during postharvest transportation and storage, posing a great threat to tomato production and postharvest preservation

Method used

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  • A kind of aroma-producing fungus and its application
  • A kind of aroma-producing fungus and its application
  • A kind of aroma-producing fungus and its application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] 1. Isolation and cultivation of aroma-producing fungi

[0027] The aroma-producing fungus was isolated from dead branches of Broussonetia papyrifera, which were collected from the Yangshan Campus of Xinyang Agriculture and Forestry University. The specific isolation and cultivation steps are as follows:

[0028] a. Take 4-5cm dead branches of the mulberry tree, rinse them with tap water for 3 minutes, then disinfect them with 75% alcohol on a sterile table for 3 minutes, rinse them with sterile water three times, and then use pointed tweezers to pick up the mulberry tree The small black dot-shaped conidia on the dead branch are put on the PDA (the streptomycin of 0.3g is added for every liter of PDA) containing streptomycin made in advance on the plate, and each plate is evenly placed at 5 points;

[0029] b. Put the above PDA plate into a 25°C incubator for cultivation, and regularly record the growth of the isolate on the plate every day;

[0030] c. After 3-4 days,...

Embodiment 2

[0038] 1. The fumigation inhibitory effect of aroma-producing fungus GS-1 strain on plant pathogenic fungi

[0039]This test example is determined by the buckle method. The steps are: use the GS-1 strain that has been cultured for 5 days under the condition of 25°C as the aroma-producing strain to be tested; Bipolaris oryzae, Fusarium graminearum, Alternaria brassicae and Sclerotinia sclerotiorum were inoculated on PDA plates with the tested aroma-producing fungus GS-1, and cultured at 25°C for 7 days , take a bacterium cake with a diameter of 5mm at the edge of the colony of five kinds of phytopathogenic fungi and the tested aroma fungus GS-1 strain; Butt button; take the petri dish only inoculated with phytopathogenic fungi as a contrast, observe the growth of phytopathogenic fungi every 24 hours, observe for 5 days, and measure the colony diameter of pathogenic fungi by the cross method, the calculation formula is: mycelial growth inhibition rate = (control colony growth d...

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Abstract

The invention relates to an aroma-producing fungus and its application, belonging to the field of microbes, and the aroma-producing fungus is Tumorella vulgaris Tubercularia vulgaris GS‑1, depository unit: China Type Culture Collection Center, deposit date: July 4, 2016, deposit number: CCTCC NO.M 2016369, this aroma-producing fungus can be used to control tomato gray mold, rice flax spot, Wheat head blight, cabbage black spot, lettuce sclerotinia and tomato fruit gray mold, thus broadening the research ideas for the prevention and control of various plant diseases, and solving the problems in the development and utilization of biocontrol agents in my country to a certain extent. limits.

Description

technical field [0001] The invention belongs to the field of microorganisms, and in particular relates to an aroma-producing fungus and its application. Background technique [0002] Fungal volatile compounds (volatile organic compounds, VOCs) refer to the mixture of various carbon-containing gaseous compounds produced by fungi, which have the characteristics of small molecular weight, volatile, and free diffusion in air and soil. Because of its unique application value in plant disease control, it has attracted the attention and attention of scholars at home and abroad. The research and use of different VOCs to control plant diseases has become a hot spot in current research at home and abroad. For example, fumigation is used to prevent and control greenhouse crop diseases, soil-borne diseases, post-harvest diseases of fruits and vegetables, and mildew of stored grains. It has been reported that the volatiles of Muscodor, Phomosis, Nodulisporium, Oxyporus, Trichoderma, Api...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/14A01N63/04A01P3/00C12R1/645
CPCA01N63/30C12N1/145C12R2001/645
Inventor 陈利军陈月华智亚楠王国君张方梅郭世保
Owner XINYANG AGRI & FORESTRY UNIV
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