Preparation method and application of brewing enhanced bacterial source

A technology for the preparation of bacterial sources and culture medium, applied in the preparation of alcoholic beverages, fungi, etc., can solve the problems of difficulty in exerting a synergistic effect, complicated operation process, etc., and achieve the effects of simple preparation method, less dosage and improved wine quality.

Inactive Publication Date: 2017-11-14
张娟 +2
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] The present invention aims to solve the complicated operation process of the preparation method in the prior art, and the addition of various chemical reagents and the content of a certain kind of pit mud functional bacteria in the preparation process will break the balance of the original microbial community during use. growth, it is difficult to exert a good synergistic effect, and a kind of wine-making strengthening bacteria source and its preparation method are provided. Mud culture, rejuvenation and enhancement of microbial degradation in pit mud, and adding wine-enhancing bacteria sources to mother's grains can quickly strengthen the microbial flora in the original environment, thereby improving the quality of wine

Method used

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  • Preparation method and application of brewing enhanced bacterial source
  • Preparation method and application of brewing enhanced bacterial source
  • Preparation method and application of brewing enhanced bacterial source

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] A preparation method for wine-enhancing bacteria source, said method comprising the steps of:

[0027] A. Culture medium preparation: mix 8.5 parts of medium bran, 0.5 parts of fermented grains, and 4.5 parts of water, adjust the pH to 6.5, sterilize in a portable pressure cooker for 43 minutes, take out the buckle bottle, cool down to 35°C, and obtain the culture medium for use;

[0028] B. Enrichment of strains in pit mud: put 150 parts of high-quality old pit mud in the culture medium in step A, and control the temperature at 32-35°C. Take out the enhanced culture medium and dry it at 70°C to obtain the first-grade strains;

[0029] C. Hierarchical and expanded cultivation: the grade-1 strains obtained in step B are subjected to hierarchical and enlarged cultivation, and after cultivation, they are dried at 70° C. together with the colonies and the culture medium to obtain the source of wine-enhancing strains.

Embodiment 2

[0031] A preparation method for wine-enhancing bacteria source, said method comprising the steps of:

[0032] A. Culture medium preparation: mix 9.5 parts of medium bran, 0.5 parts of fermented grains, and 4.5 parts of water, adjust the pH to 6.5, sterilize in a portable pressure cooker for 45 minutes, take out the buckle bottle, cool down to 35°C, and obtain the culture medium for later use;

[0033] B. Enrichment of strains in pit mud: put 100 parts of high-quality old pit mud in the culture medium in step A, and control the temperature at 32-35°C. Take out the enhanced culture medium and dry it at 50°C to obtain the first-grade strain;

[0034] C. Grading expansion culture: the grade-1 strains obtained in step B are subjected to hierarchical expansion culture, and after culture, they are dried at 50° C. together with colonies and medium to obtain wine-making strengthening bacteria sources.

[0035] In step B, when the high-quality old pit mud is placed in the culture mediu...

Embodiment 3

[0040] A preparation method for wine-enhancing bacteria source, said method comprising the steps of:

[0041]A. Culture medium preparation: mix 11 parts of medium bran, 1 part of fermented grains, and 5 parts of water, adjust the pH to 6.5, sterilize in a portable pressure cooker for 42 minutes, take out the buckle bottle, cool down to 35°C, and obtain the culture medium for later use;

[0042] B. Enrichment of strains in pit mud: put 120 parts of high-quality old pit mud into the culture medium in step A, and control the temperature at 32-35°C. Take out the enhanced culture medium and dry it at 60°C to obtain the first-grade strain;

[0043] C. Hierarchical and expanded cultivation: the grade-1 strains obtained in step B are subjected to hierarchical and enlarged cultivation, and after cultivation, they are dried at 60°C together with colonies and culture medium to obtain the source of wine-enhancing bacteria.

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Abstract

The invention provides a method for preparing wine making reinforcement bacterium sources and application thereof. The method includes steps of A, preparing cultivation media, to be more specific, mixing medium bran, fermented grains and water with one another to obtain mixtures, regulating the mixtures until the PH (potential of hydrogen) of the mixtures reaches 6.5, sterilizing the mixtures and cooling the mixtures until the temperatures of the mixtures reach 35 DEG C to obtain the cultivation media; B, gathering bacteria in pit mud, to be more specific, cultivating the high-quality aged pit mud in the cultivation media obtained at the step A at the temperature of 32-35 DEG C for 100-150 days, then fetching the cultivation media with large quantities of gathered microbial bacterial colonies and drying the cultivation media to obtain first-grade bacteria; C, carrying out fractional enlarged cultivation on the first-grade bacteria obtained at the step B and then drying the first-grade bacteria, the bacterial colonies and the cultivation media to obtain the wine making reinforcement bacterium sources. The method for preparing the wine making reinforcement bacterium source and the application have the advantages that the method is simple, chemical reagents can be omitted, the wine making reinforcement bacterium sources are applied to parent grains, new pit mud cultivation and rejuvenation and reinforcement of degenerated microorganisms in pit mud, microbial bacterial colonies in original environments can be quickly reinforced, and accordingly the quality of wine can be improved.

Description

technical field [0001] The invention relates to a wine-making strengthening bacterial source used in wine-making, in particular to a preparation method and application of a wine-making strengthening bacterial source, and belongs to the technical field of liquor brewing. Background technique [0002] At present, the majority of Luzhou-flavor winemaking enterprises mainly improve the quality of pit mud through the following two methods: First, adopt traditional pit mud cultivation and maintenance methods, that is, use winemaking resources to cultivate pit mud artificially, and rely on traditional winemaking techniques to carry out pit pit mud quality. Conservation, through multiple rounds of fermentation, gradually improve the quality of pit mud. This method is effective on the whole, but the pit mud has a longer maturity period and is slower. Second, by putting caproic acid bacteria culture solution into the pit mud to increase the content of the main functional bacteria-cap...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/16C12N1/14C12G3/02
CPCC12G3/02C12N1/14C12N1/16
Inventor 张娟张齐裕
Owner 张娟
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