Preparation method and application of brewing enhanced bacterial source
A technology for the preparation of bacterial sources and culture medium, applied in the preparation of alcoholic beverages, fungi, etc., can solve the problems of difficulty in exerting a synergistic effect, complicated operation process, etc., and achieve the effects of simple preparation method, less dosage and improved wine quality.
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Embodiment 1
[0026] A preparation method for wine-enhancing bacteria source, said method comprising the steps of:
[0027] A. Culture medium preparation: mix 8.5 parts of medium bran, 0.5 parts of fermented grains, and 4.5 parts of water, adjust the pH to 6.5, sterilize in a portable pressure cooker for 43 minutes, take out the buckle bottle, cool down to 35°C, and obtain the culture medium for use;
[0028] B. Enrichment of strains in pit mud: put 150 parts of high-quality old pit mud in the culture medium in step A, and control the temperature at 32-35°C. Take out the enhanced culture medium and dry it at 70°C to obtain the first-grade strains;
[0029] C. Hierarchical and expanded cultivation: the grade-1 strains obtained in step B are subjected to hierarchical and enlarged cultivation, and after cultivation, they are dried at 70° C. together with the colonies and the culture medium to obtain the source of wine-enhancing strains.
Embodiment 2
[0031] A preparation method for wine-enhancing bacteria source, said method comprising the steps of:
[0032] A. Culture medium preparation: mix 9.5 parts of medium bran, 0.5 parts of fermented grains, and 4.5 parts of water, adjust the pH to 6.5, sterilize in a portable pressure cooker for 45 minutes, take out the buckle bottle, cool down to 35°C, and obtain the culture medium for later use;
[0033] B. Enrichment of strains in pit mud: put 100 parts of high-quality old pit mud in the culture medium in step A, and control the temperature at 32-35°C. Take out the enhanced culture medium and dry it at 50°C to obtain the first-grade strain;
[0034] C. Grading expansion culture: the grade-1 strains obtained in step B are subjected to hierarchical expansion culture, and after culture, they are dried at 50° C. together with colonies and medium to obtain wine-making strengthening bacteria sources.
[0035] In step B, when the high-quality old pit mud is placed in the culture mediu...
Embodiment 3
[0040] A preparation method for wine-enhancing bacteria source, said method comprising the steps of:
[0041]A. Culture medium preparation: mix 11 parts of medium bran, 1 part of fermented grains, and 5 parts of water, adjust the pH to 6.5, sterilize in a portable pressure cooker for 42 minutes, take out the buckle bottle, cool down to 35°C, and obtain the culture medium for later use;
[0042] B. Enrichment of strains in pit mud: put 120 parts of high-quality old pit mud into the culture medium in step A, and control the temperature at 32-35°C. Take out the enhanced culture medium and dry it at 60°C to obtain the first-grade strain;
[0043] C. Hierarchical and expanded cultivation: the grade-1 strains obtained in step B are subjected to hierarchical and enlarged cultivation, and after cultivation, they are dried at 60°C together with colonies and culture medium to obtain the source of wine-enhancing bacteria.
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