Rooting and seedling strengthening method for tissue culture seedling of gerbera jamesonii
A technology for tissue culture seedlings, strong seedlings and rooting, applied in the field of plant tissue culture, can solve the problems of reduced survival rate of gerbera tissue culture seedlings transplanted, affecting the promotion of new varieties of seedling offspring, unable to maintain the excellent characteristics of the mother plant, and the like. The effect of improving the productivity of tissue culture seedlings, improving the survival rate of transplanting and shortening the recovery time
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Embodiment 1
[0033] Embodiment 1 carries out rooting and hardening of gerbera tissue culture seedlings in summer, mainly comprising the following steps:
[0034] 1) In mid-to-late July, cut the clustered tissue-cultured seedlings derived from multiplication and cultured gerbera into single buds, transfer them to a culture bottle equipped with strong seedling rooting medium, cover the bottle cap, and carry out light culture to induce rooting, The temperature is 25±2°C, the light intensity is about 2500lux, the rooting culture is 25-30 days, 100% of the tissue culture seedlings are rooted, and the rooted gerbera tissue culture seedlings are obtained;
[0035] Wherein, the rooting medium for strong seedlings is: 1 / 2MS+6-BA 0.1mg / L+IBA0.3mg / L+NAA 0.1mg / L+pp333 0.1mg / L+sucrose 20g / L+agar powder 6.0g / L L;
[0036] 2) Keep the gerbera plantlets rooted in step 1) without opening the bottle cap, and keep them in the culture room for 2 months, with light intensity of 1500-2000 lux, and temperature ...
Embodiment 2
[0040] Embodiment 2 carries out rooting and hardening of gerbera tissue culture seedlings in winter, mainly comprising the following steps:
[0041] 1) In mid-November, cut the clustered tissue-cultured seedlings derived from multiplication and cultured gerbera into single buds, transfer them to a culture bottle with strong seedling rooting medium, cover the bottle cap, and carry out light culture to induce rooting. 25±2°C, light intensity of about 2500lux, rooting culture for 25-30 days, 100% tissue culture seedlings take root, and rooted gerbera tissue culture seedlings are obtained;
[0042] Wherein, the rooting medium for strong seedlings is: 1 / 2MS+6-BA 0.05mg / L+IBA0.5mg / L+NAA0.05mg / L+pp333 0.1mg / L+sucrose 20g / L+agar powder 6.0g / L L;
[0043]2) Put the rooted gerbera tissue culture seedlings in step 1) without opening the bottle cap and place them in the greenhouse for 2.5 months to harden the seedlings, the light intensity is about 3000 lux, and the temperature is 10-26 ...
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