Rooting and seedling strengthening method for tissue culture seedling of gerbera jamesonii

A technology for tissue culture seedlings, strong seedlings and rooting, applied in the field of plant tissue culture, can solve the problems of reduced survival rate of gerbera tissue culture seedlings transplanted, affecting the promotion of new varieties of seedling offspring, unable to maintain the excellent characteristics of the mother plant, and the like. The effect of improving the productivity of tissue culture seedlings, improving the survival rate of transplanting and shortening the recovery time

Inactive Publication Date: 2016-11-09
SHANGHAI ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

This technology can overcome the shortcomings of conventional means such as slow propagation of seedlings, affecting the promotion of new varieties, and the offspring of seedlings will mutate and cannot maintain the excellent characteristics of the mother plant.
[0004] Tissue culture technology is an important method for rapid propagation of gerbera seedlings. However, the artificial planting of tissue culture seedlings is a process from heterotrophic to autotrophic, and there are still problems of high management level requirements and low transplant survival rate. The most outstanding thing is that, affected by the season, when transplanting in summer and winter, the transplanting survival rate of gerbera tissue culture seedlings is greatly reduced
[0005] In order to overcome the defect of low survival rate of gerbera seedlings ...

Method used

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  • Rooting and seedling strengthening method for tissue culture seedling of gerbera jamesonii
  • Rooting and seedling strengthening method for tissue culture seedling of gerbera jamesonii

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Experimental program
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Embodiment 1

[0033] Embodiment 1 carries out rooting and hardening of gerbera tissue culture seedlings in summer, mainly comprising the following steps:

[0034] 1) In mid-to-late July, cut the clustered tissue-cultured seedlings derived from multiplication and cultured gerbera into single buds, transfer them to a culture bottle equipped with strong seedling rooting medium, cover the bottle cap, and carry out light culture to induce rooting, The temperature is 25±2°C, the light intensity is about 2500lux, the rooting culture is 25-30 days, 100% of the tissue culture seedlings are rooted, and the rooted gerbera tissue culture seedlings are obtained;

[0035] Wherein, the rooting medium for strong seedlings is: 1 / 2MS+6-BA 0.1mg / L+IBA0.3mg / L+NAA 0.1mg / L+pp333 0.1mg / L+sucrose 20g / L+agar powder 6.0g / L L;

[0036] 2) Keep the gerbera plantlets rooted in step 1) without opening the bottle cap, and keep them in the culture room for 2 months, with light intensity of 1500-2000 lux, and temperature ...

Embodiment 2

[0040] Embodiment 2 carries out rooting and hardening of gerbera tissue culture seedlings in winter, mainly comprising the following steps:

[0041] 1) In mid-November, cut the clustered tissue-cultured seedlings derived from multiplication and cultured gerbera into single buds, transfer them to a culture bottle with strong seedling rooting medium, cover the bottle cap, and carry out light culture to induce rooting. 25±2°C, light intensity of about 2500lux, rooting culture for 25-30 days, 100% tissue culture seedlings take root, and rooted gerbera tissue culture seedlings are obtained;

[0042] Wherein, the rooting medium for strong seedlings is: 1 / 2MS+6-BA 0.05mg / L+IBA0.5mg / L+NAA0.05mg / L+pp333 0.1mg / L+sucrose 20g / L+agar powder 6.0g / L L;

[0043]2) Put the rooted gerbera tissue culture seedlings in step 1) without opening the bottle cap and place them in the greenhouse for 2.5 months to harden the seedlings, the light intensity is about 3000 lux, and the temperature is 10-26 ...

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Abstract

The invention discloses a rooting and seedling strengthening method for tissue culture seedlings of gerbera jamesonii. The rooting and seedling strengthening method comprises the following steps: cutting clustered tissue culture seedlings of gerbera jamesonii into single buds, performing lighting culture on a seedling strengthening rooting culture medium, inducing rooting, strengthening the seedlings in covered bottles for 1-3 months in a greenhouse or a culture room after rooting, gradually opening the bottles to strength the seedlings, and performing heel-in in a cultivation medium. By adopting the seedling strengthening rooting culture medium, sufficient nutrition can be provided, the growth speed of the tissue culture seedlings of gerbera jamesonii can be appropriately reduced, the maintenance and management time of the tissue culture seedlings of gerbera jamesonii on a seedbed can be shortened, production of the tissue culture seedlings of gerbera jamesonii is not interrupted in hot summer and cold winter, the labor and the water and power cost of seedbed management are greatly reduced, the method is simple to operate and convenient to manage, the tissue culture seedlings of gerbera jamesoni, which is cultured by using the method, are good in root system development, the survival rate after heel-in is greater than 99%, and the seedlings can all survive after being transplanted fixedly.

Description

technical field [0001] The invention belongs to the technical field of plant tissue culture, and in particular relates to a method for rooting and hardening gerbera tissue culture seedlings. Background technique [0002] Gerbera jamesonii (Gerbera jamesonii) is a perennial herbaceous plant of the genus Gerbera in the Compositae family. It has the advantages of rich flower colors, high flower yield, and long ornamental period. It is one of the top five cut flowers in the world and is widely used in potted plants and fresh cut flowers. [0003] With the continuous development of the flower market, the amount of seedlings used for gerbera production is increasing day by day, and the rapid propagation of gerbera seedlings using tissue culture technology has achieved rapid development. This technology can overcome the shortcomings of conventional means such as slow propagation of seedlings, affecting the promotion of new varieties, and the offspring of seedlings will mutate and c...

Claims

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Application Information

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IPC IPC(8): A01H4/00
CPCA01H4/001A01H4/008
Inventor 殷丽青孙翊蔡友铭张永春陆锦明杨柳燕李水根朱天华李心
Owner SHANGHAI ACAD OF AGRI SCI
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