Immobilized algae-bacterium spheres and preparation method and application thereof
A technology for algal balls and algal bacteria, which is applied to the field of immobilized algal balls and their preparation, can solve the problems of the quality decline of aquaculture products, pollution of aquaculture water environment, frequent occurrence of diseases, etc., and achieve large-scale industrial production, good quality Purification effect, low cost effect
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Embodiment 1
[0026] Use 100mL graduated cylinders to measure 80mL cultured 10 6 Individual / ml Chlorella pyrenoidosa and 80mL of 10 8 photosynthetic bacteria per ml, centrifuge with NF1200 desktop centrifuge, 3000 rpm, centrifuge for 6min, remove the supernatant. Dilute to 1 mL with deionized water. Thoroughly mix 3 mL of Chlorella pyrenoidosa and 1 mL of photosynthetic bacteria. Add 3mL of chlorella pyrenoidosa, 1mL of photosynthetic bacteria, and 4mL of the algae-bacteria mixture into 20mL of fully dissolved 2% sodium alginate solution with a pipette, and stir for 2 hours with a glass rod to make them evenly mixed. Use a 20mL syringe to draw 2% sodium alginate solution mixed with algae, bacteria, and algae, install a No. 16 needle, and drop them into 100mL pre-cooled 6% calcium chloride solution at a uniform speed to form immobilized algal balls. The pellets were crosslinked in calcium chloride solution at room temperature for 24 hours to obtain an average radius of 2mm and an average ...
Embodiment 2
[0028] The immobilized algae bacterium ball in embodiment 1 is put into NH 4 Cl, NaNO 2 and K 2 HPO 4 Formulated with NH 4 + The N content in the medium is 10mg / L, NO 2 + In the culture medium with N content of 1.2mg / L and P content of 5mg / L, the stocking density was 2.8×10 5 piece / m 3 , cultured for 4 days at a temperature of 25°C, a light intensity of 3000Lx, and a light-dark ratio of 12h:12h to obtain domesticated immobilized algae balls. The algae concentration increased by 3.2 times, and the bacteria concentration increased by 6.8 times. The ball adapts to the delivery environment so as to facilitate delivery.
Embodiment 3
[0030] Shanghai Songjiang aquaculture pond, before use, the soluble TP of the water body was 5mg / L, the ammonia nitrogen was 10mg / L, and the COD was 80mg / L. The immobilized algae balls in Example 1 were used, and the stocking density was 2×10 5 piece / m 3 , each of which is 0.04±0.01g. After use, the water-soluble TP is 1.8mg / L, ammonia nitrogen is 1.2mg / L, and COD is 20mg / L.
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