Immobilized algae-bacterium spheres and preparation method and application thereof

A technology for algal balls and algal bacteria, which is applied to the field of immobilized algal balls and their preparation, can solve the problems of the quality decline of aquaculture products, pollution of aquaculture water environment, frequent occurrence of diseases, etc., and achieve large-scale industrial production, good quality Purification effect, low cost effect

Inactive Publication Date: 2016-11-09
FISHERY MACHINERY & INSTR RES INST CHINESE ACADEMY OF FISHERY SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, due to a series of problems such as a large amount of bait input, a large amount of medication and a large number of water changes in the breeding process, the pollution of the aquaculture water environment has become increasingly serious, the deterioration of the aquaculture environment has caused frequent occurrence of diseases, and the quality of aquaculture products has declined.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] Use 100mL graduated cylinders to measure 80mL cultured 10 6 Individual / ml Chlorella pyrenoidosa and 80mL of 10 8 photosynthetic bacteria per ml, centrifuge with NF1200 desktop centrifuge, 3000 rpm, centrifuge for 6min, remove the supernatant. Dilute to 1 mL with deionized water. Thoroughly mix 3 mL of Chlorella pyrenoidosa and 1 mL of photosynthetic bacteria. Add 3mL of chlorella pyrenoidosa, 1mL of photosynthetic bacteria, and 4mL of the algae-bacteria mixture into 20mL of fully dissolved 2% sodium alginate solution with a pipette, and stir for 2 hours with a glass rod to make them evenly mixed. Use a 20mL syringe to draw 2% sodium alginate solution mixed with algae, bacteria, and algae, install a No. 16 needle, and drop them into 100mL pre-cooled 6% calcium chloride solution at a uniform speed to form immobilized algal balls. The pellets were crosslinked in calcium chloride solution at room temperature for 24 hours to obtain an average radius of 2mm and an average ...

Embodiment 2

[0028] The immobilized algae bacterium ball in embodiment 1 is put into NH 4 Cl, NaNO 2 and K 2 HPO 4 Formulated with NH 4 + The N content in the medium is 10mg / L, NO 2 + In the culture medium with N content of 1.2mg / L and P content of 5mg / L, the stocking density was 2.8×10 5 piece / m 3 , cultured for 4 days at a temperature of 25°C, a light intensity of 3000Lx, and a light-dark ratio of 12h:12h to obtain domesticated immobilized algae balls. The algae concentration increased by 3.2 times, and the bacteria concentration increased by 6.8 times. The ball adapts to the delivery environment so as to facilitate delivery.

Embodiment 3

[0030] Shanghai Songjiang aquaculture pond, before use, the soluble TP of the water body was 5mg / L, the ammonia nitrogen was 10mg / L, and the COD was 80mg / L. The immobilized algae balls in Example 1 were used, and the stocking density was 2×10 5 piece / m 3 , each of which is 0.04±0.01g. After use, the water-soluble TP is 1.8mg / L, ammonia nitrogen is 1.2mg / L, and COD is 20mg / L.

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Abstract

The invention relates to immobilized algae-bacterium spheres and a preparation method, a culture method and an application thereof. The immobilized algae-bacterium spheres are composed of chlorella pyrenoidosa, photosynthetic bacteria and embedding materials. The preparation method of the immobilized algae-bacterium spheres mainly comprises the steps of adding dropwise the cultured chlorella pyrenoidosa and photosynthetic bacteria into a sodium alginate solution according to a certain ratio, performing full stirring with a glass rod so as to enable the materials to be uniformly mixed, adding dropwise the obtained mixture into a calcium chloride solution, and performing cross-linking to obtain the immobilized algae-bacterium spheres. The prepared immobilized algae-bacterium spheres by the invention are good in effects of purifying aquaculture discharged water, and have purifying functions of algae and bacteria, double purification effects, and certain density and mechanical strength, and is not easy to break, and easy to sink to the water bottom, and can play a better purification effect. The invention further provides a continuous cultivation mode of the immobilized algae-bacterium spheres, which is simple and easy to operate, low in cost, and can realize large-scale industrial production.

Description

technical field [0001] The invention relates to an immobilized algae ball and its preparation method and application. Background technique [0002] With the growth of the world's population and the development of science and technology, fishery resources that were once called "inexhaustible" have become increasingly scarce. In order to meet human demand for high-quality protein, major fishery countries have paid more attention to aquaculture, making it quickly become one of the fastest-growing industries in the world's food production. In particular, my country's aquaculture industry has seen the fastest growth in global animal food production in recent years. However, due to a series of problems such as a large amount of bait input, a large amount of medication and a large number of water changes in the breeding process, the pollution of the aquaculture water environment has become increasingly serious, the deterioration of the aquaculture environment has caused frequent d...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N11/10C12N11/04C02F3/34C12R1/645C02F103/20
CPCC12N11/10C02F3/34C02F2103/20C12N11/04
Inventor 刘娥刘兴国陆诗敏曾宪磊王小冬时旭
Owner FISHERY MACHINERY & INSTR RES INST CHINESE ACADEMY OF FISHERY SCI
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