DNA Structure Probe Based on Nucleic Acid Isothermal Amplification Technology to Detect Mercury Ions

Abstract

DNA structure probes based on nucleic acid constant temperature amplification technology to detect mercury ions, including nucleic acid sequences of three DNA structure probes that contain mismatch sites and can form a triple hybridization structure through nucleic acid hybridization. The triple hybridization structure is characterized by being triggered by mercury ions The DNA probe structure is formed by hybridization, which spatially separates the mercury ion binding region and the polymerase recognition region, and uses the rapid polymerization of the polymerase to obtain a stable structure for sustainable polymerization before the biothiol molecule destroys the interaction between mercury ions and thymine , cleverly avoided the interference of biothiol molecules. This method can effectively eliminate the loss of trace mercury ions due to the interference of biothiol molecules when the nucleic acid constant temperature method is used to detect, effectively improve the detection efficiency of trace mercury ions without introducing any reagents, and further improve the detection efficiency of nucleic acid. Application of isothermal amplification technology in the detection of mercury ions.

Description

technical field [0001] The invention belongs to the trace detection of mercury ions in the field of biotechnology, in particular to a DNA structure probe for detecting mercury ions based on nucleic acid constant temperature amplification technology. [0002] technical background [0003] Heavy metal ions have high stability in the environment and are difficult to be degraded by microorganisms. Once they enter the environment, they are difficult to be repaired naturally, and can be continuously enriched and passed on in the ecosystem. Even a small amount of ingestion can cause great toxicity, which is a major threat to ecological balance and human health. As a typical representative of heavy metal ion pollution, the accumulation of mercury ions in biological tissues causes DNA damage, affects ligand-receptor interactions, destroys the immune system, and causes a series of diseases, such as brain damage, kidney failure, various cognition and movement disorders. Therefore, the...

AI Technical Summary

Problems solved by technology

The existence of biothiol molecules will compete with thymine to capture trace mercury ions in the system, resulting in a decrease in the interaction between mercury ions and thymine, resulting in low detection sensitivity of trace mercury ions, which affects the nucleic acid constant temperature amplification method. detection applications

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