47results about How to "Increase the cost of experiments" patented technology

The invention discloses a method to screen and culture barley transfer-gene plant with non-antibiotic of agricillin dielectric conductance in plant transfer-gene technical domain, which comprises thefollowing steps: leading reported gene into receptor barley cell through agricillin dielectric conductance; utilizing mannose to replace antibiotic as chosen agent; screening the reverting receptor cell; adjusting the genetic reforming process of barley through phosphomannose isomerase; further-screening transfer-gene barley with chlorophenol red colouration method and PCR method; getting the endproduct. The barley cultivating strain conversion rate can reach 12% with agricillin dielectric conductance and can reach 100% with chlorophenol red colouration method.

The invention relates to an ion sensor for detecting thiocyanate by naked eyes according to color change and a preparation method and application of the ion sensor. The ion sensor comprises a first plasma resonance metal level, a PMETAC molecular brush and a second plasma resonance metal level, the first and second plasma resonance metal levels are arranged in the two sides of the PMETAC molecularbrush respectively to form an MIM type resonator, and the first and second plasma resonance metal levels include component of gold, silver, platinum or aluminum. According to ion sensor and the preparation method and application thereof, mark-free test can be realized, a test means is convenient and economical, the reutilization rate is high, the anti-interference performance is high, response time is short, and the ion sensor is suitable for real-time detection.

The invention discloses a coal and rock bore hydraulic fracture experimental apparatus, which comprises a sample device system, a confining pressure system, a fracture system, a sealing system and a monitoring system, wherein the sample device system comprises a sample cylinder, the bottom in the sample cylinder is provided with a screen drum of which the bottom is provided with a water outlet pipe, a sample is accommodated in the sample cylinder above the screen drum, and a simulated bore is formed in the sample; the confining pressure system comprises an air filling subsystem and a water filling subsystem; the fracture system comprises a fracture high-pressure water pump of which a water outlet is communicated with the simulated bore through a fracture pipeline; the sealing system comprises a sample cylinder sealing device and an experimental cylinder sealing device; and the monitoring system comprises an acoustic emission sensor arranged on an external wall of the sample cylinder. By the coal and rock bore hydraulic fracture experimental apparatus, a simulated experiment can be performed in a laboratory to investigate the change rules of crack initiation, expansion and extension in the hydraulic fracture process of a coal mine underground coal seam bore, and know a crack extending direction so as to guide field fracture; therefore, the fracture effect is greatly improved.

The invention relates to a butyl phthalide tablet and a preparation method thereof. The butyl phthalide tablet is composed of butyl phthalide solid powders and other medical troche auxiliary materials; wherein, the butyl phthalide solid powders are composed of the components with the following weight percentage: 9 percent to 30 percent of butyl phthalide, 0.01 percent to 1.2 percent of emulsifiers and 70 percent to 90 percent of cyclodextrin or cyclodextrin ramification. The troche which is made with the invention has favorable forming, stable product quality and high dissolution rate.

The invention discloses a method for detecting the quality of sorbic acid (potassium). The sorbic acid (potassium) to be tested is dried, and the difference between the light transmittance before drying and the light transmittance after drying is The thermal stability of sorbic acid, the smaller the thermal stability difference, the better the quality of sorbic acid (potassium) to be tested, the more stable the product, and the longer the shelf life.

The invention provides a method for detecting protein based on a nanogold-DNA composite material, comprising the steps: preparing the nanogold-DNA composite, producing a nanogold-DNA-protein ternary composite, catalyzing nitrophenol and sodium borohydride with nanogold for reaction (a color development system), visually inspecting the period of time during which the color of reaction liquid changes from yellow to colorless (CCT value), drawing an LDA chart according to the CCT / CCT0 values of different protein, adding protein with given concentration to be detected into the color development system, calculating the CCT / CCT0 values of the protein to be detected, and identifying the protein to be detected with combination of the LDA chart. The method is established on the basis of a linear distinguishing and analysis technology and can successfully identify different protein only through visually inspecting and recording with a timer the period of time during which the color of the liquid changes, no expensive instrument is needed, the detection limit achieves 30nM, and a new technological means is provided for high flux quick detection of protein.

The invention discloses a refrigeration equipment rotor compressor load excitation recognition method under the time-varying working condition. The method is characterized in that vibration testing is performed on a refrigeration equipment rotor compressor exhaust muffler opening under the time-varying condition, an actual measurement acceleration response time history signal of the rotor compressor exhaust muffler opening is obtained, and an acceleration expected response matrix is constructed; a finite element method is used for constructing a refrigeration equipment rotor compressor virtual model machine model, the virtual model machine model is applied to analyzing acceleration response of an exhaust muffler opening of the refrigeration equipment rotor compressor under the effect of white noise loads, and a transfer function matrix is obtained. Back calculation is performed on the result of vibration testing of the rotor compressor exhaust muffler opening measured point under the time-varying condition to perform load excitation of the refrigeration equipment rotor compressor under the time-varying condition, and an iterative method is adopted for eliminating errors. Refrigeration equipment rotor compressor load excitation recognition analysis under the time-varying condition is achieved, and the basis is provided for the compressor-pipeline system vibration analysis and pipeline structure reliability design, estimation and the like.

The invention relates to real-time fluorescent quantitative PCR primers, a probe and a kit for detecting tupaia adenoviruses and belongs to the field of biotechnology. The primers are designed and synthesized according to the end 3' conserved sequence of the tupaia whole genome isolated and identified in this laboratory. The primers do not undergo a cross reaction with a tupaia source and common viruses belonging to other species. The end 5' of the probe is connected to a fluorescent reporting group and the end 3' of the probe is connected to a fluorescence quenching group. The kit produces accurate and reliable results, has good stability, good repeatability and high sensitivity, can fast and accurately quantify, has a wide detection range, can be used for epidemiological investigation caused by tupaia adenoviruses, provides the technical support for related basic research and has a wide application prospect.

DNA structure probes based on nucleic acid constant temperature amplification technology to detect mercury ions, including nucleic acid sequences of three DNA structure probes that contain mismatch sites and can form a triple hybridization structure through nucleic acid hybridization. The triple hybridization structure is characterized by being triggered by mercury ions The DNA probe structure is formed by hybridization, which spatially separates the mercury ion binding region and the polymerase recognition region, and uses the rapid polymerization of the polymerase to obtain a stable structure for sustainable polymerization before the biothiol molecule destroys the interaction between mercury ions and thymine , cleverly avoided the interference of biothiol molecules. This method can effectively eliminate the loss of trace mercury ions due to the interference of biothiol molecules when the nucleic acid constant temperature method is used to detect, effectively improve the detection efficiency of trace mercury ions without introducing any reagents, and further improve the detection efficiency of nucleic acid. Application of isothermal amplification technology in the detection of mercury ions.