47results about How to "Increase the cost of experiments" patented technology

The invention discloses a method for reconstructing a digital rock core and pore network model based on a random fractal theory. The method comprises the following steps of based on the multiple fractal features of a porous medium, performing fractal representing on a capillary pressure curve obtained by the conventional mercury intrusion method, inferring fractal expressions of random distribution density function, average value and variance by a random distribution theory, and combining the random theory and the multiple fractal theory to construct a digital rock core, so as to rapidly construct a three-dimensional micro-network model. The method has the advantages that the cost is low and is reduced; the capillary pressure curve is measured by the mercury intrusion method, the full-section rock core is used in experiment, and a micro-pore structure is fully displayed. According to the method, the computing method is simple and convenient, and the advanced method is adopted; the micro-pore structure of reservoir rock is complicated and irregular, and an extremely complicated system cannot be accurately described by the traditional classical theory, so after the method combines the multiple fractal theory and the random distribution theory, the micro-pore structure can be accurately represented, and the method is easily implemented by programming.

The invention discloses a coal and rock bore hydraulic fracture experimental apparatus, which comprises a sample device system, a confining pressure system, a fracture system, a sealing system and a monitoring system, wherein the sample device system comprises a sample cylinder, the bottom in the sample cylinder is provided with a screen drum of which the bottom is provided with a water outlet pipe, a sample is accommodated in the sample cylinder above the screen drum, and a simulated bore is formed in the sample; the confining pressure system comprises an air filling subsystem and a water filling subsystem; the fracture system comprises a fracture high-pressure water pump of which a water outlet is communicated with the simulated bore through a fracture pipeline; the sealing system comprises a sample cylinder sealing device and an experimental cylinder sealing device; and the monitoring system comprises an acoustic emission sensor arranged on an external wall of the sample cylinder. By the coal and rock bore hydraulic fracture experimental apparatus, a simulated experiment can be performed in a laboratory to investigate the change rules of crack initiation, expansion and extension in the hydraulic fracture process of a coal mine underground coal seam bore, and know a crack extending direction so as to guide field fracture; therefore, the fracture effect is greatly improved.

The invention relates to a butyl phthalide tablet and a preparation method thereof. The butyl phthalide tablet is composed of butyl phthalide solid powders and other medical troche auxiliary materials; wherein, the butyl phthalide solid powders are composed of the components with the following weight percentage: 9 percent to 30 percent of butyl phthalide, 0.01 percent to 1.2 percent of emulsifiers and 70 percent to 90 percent of cyclodextrin or cyclodextrin ramification. The troche which is made with the invention has favorable forming, stable product quality and high dissolution rate.

The invention discloses multiplex PCR (polymerase chain reaction) detection primers for avian leukosis viruses. The primers comprise detection primers for detecting subgroup A, subgroup B and subgroup J of avian leukosis viruses. The inventional also discloses a reaction system and reaction conditions of multiplex PCR. The detection method provided by the invention has the advantages of being rapid, accurate and cheap.

The invention provides a method for inverting a low permeability reservoir non-darcy flow rule by utilizing pressure monitoring data. The method comprises the following steps: measuring a bottom hole pressure value of an oil well in a period of shut-in time so as to obtain a relational value between a pressure difference and a time difference; presetting a relation between seepage velocity and pressure gradient and expressing by using a specific function; establishing and considering a mathematical model of a dynamic permeability change relationship in a default function, setting initial values of model parameters, and solving a relationship between a theoretical bottom hole pressure difference and the time difference according to a numerical model; comparing the relations between the bottom hole pressure difference and the time difference obtained by theoretical solution and actual measurement, and if the two relations meet error requirements, taking set reservoir parameters and the coefficient in the specific function as parameters obtained by interpretation during theoretical calculation; substituting the coefficient into the preset specific function, and drawing a permeability rule curve; normalizing the permeability rule curve of each well in the same block, and finally obtaining the in-situ low permeability non-darcy flow rule curve under the reservoir scale.

The present invention provides a method used for synthesizing p methoxyacetophenone with phenol. The method comprises the following steps: an acylating agent and the phenol react to prepare phenyl acetate with a catalyst; the phenyl acetate is recomposed and separated to prepare acetyl phenol; the acetyl phenol is used for preparing the p methoxyacetophenone through methoxylation. Compared with the traditional synthetic method of the p methoxyacetophenone, the method has the characteristics of low price in producing the raw materials, mild experimental conditions, less by-products in the experiment and high yield of the product.

The invention relates to a method for detecting mercury content in polymeric materials. The method comprises the following steps: a sample is pre-treated at first, namely, (1), taking a polymeric material sample to be detected, adding sufficient or excessive concentrated sulfuric acid, heating until sulphuric acid smoke is generated, so as to dispelling and carbonizing the sample; (2), adding sufficient or excessive concentrated sulfuric acid into the sample processed in the step (1) until carbides disappear and the solution is clear, so as to obtain a solution containing mercury sulfate; (3), heating till boiling, keeping reaction until the mercury sulfate is totally converted into mercuric chloride, and cooling to obtain a sample solution to be detected; then, a detector is adopted to detect the mercury sulfate in the sample solution to be detected. According to the invention, through the adoption of the method, the adsorption and volatile loss of mercury can be avoided, the precision of the detecting result is high, batch operation is realized, and reaction phenomena can be observed, and the experimental cost is low.

The invention relates to a method for detecting content of heavy metal in a polymeric material. The method includes the following steps: pretreating a sample, namely (1), adding a sufficient or excessive amount of concentrated sulfuric acid in a to-be-detected polymeric material sample, heating until sulfuric acid smoke is generated, and enabling the sample to be decomposed and carbonized; (2), adding a sufficient or excessive amount of concentrated nitric acid in the sample after being treated in the step (1) until carbide disappears and a solution is clear so as to enable the heavy metal to fully exist in the solution in a sulfate manner; (3), adding a sufficient or excessive amount of hydrochloric acid solution in the sample after being treated in the step (2), heating till boiling and maintaining reaction until the sulfate of the heavy metal is completely converted into corresponding chloride, cooling the chloride to obtain a to-be-detected sample solution, and then adopting a detection instrument to detect the content of the heavy metal in the to-be-detected sample solution. By the method, adsorption and volatilization loss of the heavy metal can be avoided, and detection results are high in accuracy; the method is supportive of large-scale operation and visual reaction phenomenon, and low in experiment cost.

The invention discloses a refrigeration equipment rotor compressor load excitation recognition method under the time-varying working condition. The method is characterized in that vibration testing is performed on a refrigeration equipment rotor compressor exhaust muffler opening under the time-varying condition, an actual measurement acceleration response time history signal of the rotor compressor exhaust muffler opening is obtained, and an acceleration expected response matrix is constructed; a finite element method is used for constructing a refrigeration equipment rotor compressor virtual model machine model, the virtual model machine model is applied to analyzing acceleration response of an exhaust muffler opening of the refrigeration equipment rotor compressor under the effect of white noise loads, and a transfer function matrix is obtained. Back calculation is performed on the result of vibration testing of the rotor compressor exhaust muffler opening measured point under the time-varying condition to perform load excitation of the refrigeration equipment rotor compressor under the time-varying condition, and an iterative method is adopted for eliminating errors. Refrigeration equipment rotor compressor load excitation recognition analysis under the time-varying condition is achieved, and the basis is provided for the compressor-pipeline system vibration analysis and pipeline structure reliability design, estimation and the like.

The invention discloses a 2D simulation experiment method for the flowing state of soil particles in the vibrating compaction process. The 2D simulation experiment method comprises the steps of: preparing a wooden box with a transparent front wall and detachably adjustable left and right walls; preparing a group of aluminum rods with different diameters; marking different colors on the end surfaces of the aluminum rods according to the different diameters; mixing the aluminum rods according to a certain proportion; wrapping the surfaces of the aluminum rods by using gauze and fine sand to simulate an internal friction angle; simulating cohesive force by using butter or slow-drying weak-viscosity glue; manufacturing a small loading device; paving the aluminum rods in a paper box; measuring and calculating the porosity before the experiment; starting a compaction experiment; measuring and calculating the porosity after the compaction; comparing the front and rear porosities; determining the compaction effect; observing a picture obtained by a high-speed camera; and analyzing a microscopic motion mechanism. The method can conveniently and detailedly simulate the flowing state of the soil particles in the vibrating compaction process, provides convenience for explaining the vibrating compaction mechanism from a microscopic angle, and provides a visual and effective experimental means for researching the relation between microscopic motion and macroscopic parameters in the vibrating compaction process.

The invention discloses a temperature-controlled self-cleaving single plasmid homologous recombination system and application thereof in gene editing. The system comprises a single plasmid pKID220 used for gene editing, the pKID220 plasmid contains a temperature control element, a Red recombinase gene, an I-SceI endonuclease gene and a targeting fragment region containing a selection marker, and the two sides of the selection marker are both provided with an I-SceI recognition site and an FRT site. Experiments prove that the recombination efficiency of the homologous recombination system is superior to that of a double plasmid and arabinose induction method of a traditional lambda-Red system, no chemical inducer is needed, and efficient editing of chromosomes can be achieved only by changing the culture temperature. Moreover, multiple electroporation processes are avoided, the operation complexity is greatly reduced, and the time and the cost are saved. The invention provides a quicker, simpler, more convenient and more economical technical means for gene editing.

The invention discloses a method to screen and culture barley transfer-gene plant with non-antibiotic of agricillin dielectric conductance in plant transfer-gene technical domain, which comprises the following steps: leading reported gene into receptor barley cell through agricillin dielectric conductance; utilizing mannose to replace antibiotic as chosen agent; screening the reverting receptor cell; adjusting the genetic reforming process of barley through phosphomannose isomerase; further-screening transfer-gene barley with chlorophenol red colouration method and PCR method; getting the end product. The barley cultivating strain conversion rate can reach 12% with agricillin dielectric conductance and can reach 100% with chlorophenol red colouration method.

The invention discloses a method for building a model of piglets infected by haemophilus parasuis (HPS) and relates to the field of animal model building. The method includes that a plurality of colostrum-deprived (CD) piglets are raised to 18-25 days old and uniformly divided into an infected group A and a control group B, the fact that the CD piglets are free from HPS pathogens, APP pathogens or APP antibodies is determined, then the piglets are raised to 40-70 days old, HPS strains are inoculated to tracheas of all CD piglets in the infected group A, culture media are injected to the tracheas of all CD piglets in the control group B to obtain a blank group B', a toxic group A' and the blank group B' are observed and detected, total deoxyribose nucleic acid (DNA) of lung tissue of the toxic group A' and the blank group B' is extracted, and the lung tissue of the blank group B' is free from the HPS pathogens while the HPS pathogens are determined to exist in the lung tissue of the toxic group A'. By means of the method for building the model of the infected piglets by HPS, experiment costs can be reduced, the modeling process is simple and convenient, the result accuracy is high, and the method can meet relevant requirements of animal welfare.

The invention discloses application of a CTLA4 gene and PD1 gene humanized mouse model, and relates to the field of animal gene engineering and gene genetic modification. The CTLA4 and PD1 immune checkpoint humanized model with the BALB / c background is constructed and obtained, the model can reproduce toxic reactions of CTLA4 antibody drugs, PD1 antibody drugs and combined drugs, the problem thatother rodent mice cannot be subjected to toxicity evaluation is solved, meanwhile, the model is not as high as primate in experimental cost, and the model fills the market blank of PD1 and CTLA4 antibody safety evaluation of the rodents. The model constructed by adopting the model construction method can be used for evaluating the effectiveness of the targeted drug, screening and developing the targeted drug, evaluating the anti-tumor efficacy of the targeted drug in combination with other drugs, or researching the toxicology of the targeted drug.

The invention discloses a method for quantitative forecasting of lake basin landforms based on flow field representation. The method comprises the steps that major sand control geological factors are determined; according to structural characteristics of a research area, a basin is recovered according to a stratum burial history; a dynamic simulation system of a basin deposition sand body system is established based on basin recovery, and influences of material sources, hydrodynamic condition parameters and structural characteristic parameters on sand body distribution evolution are analyzed; according to different particle carrying modes, deposition substances are divided into cohesive silt and non-cohesive silt, and simulation representation of deposition compositions can be achieved; through simulation representations of the deposition compositions, three deposition conditions including deposition, denudation and no keeping after passing of the analyzed deposition substances are judged, and a deposition amount of the deposition substances is computed; quantitative representation of the deposition basin can be achieved; and based on simulation, the thickness of the deposition substances is overlaid to a bottom shape and finally projected to a horizontal plane, so the quantitative forecasting of the lake basin landforms can be achieved. The method has the advantages that different deposition environments can be simulated and analyzed, and the cost is low.

The invention belongs to the technical field of metal materials, and specifically relates to an ion sensor for detecting thiocyanate with the naked eye based on color changes and its preparation method and application. The technical solution of the present invention is as follows: an ion sensor that detects thiocyanate with the naked eye based on color changes, including a plasma resonance metal layer one, a PMETAC molecular brush and a plasma resonance metal layer two, the plasma resonance metal layer one and plasma The second resonance metal layer is arranged on both sides of the PMETAC molecular brush to form a MIM resonator; the components of the plasmon resonance metal layer 1 and the plasmon resonance metal layer 2 are gold, silver, platinum or aluminum. The invention provides an ion sensor for detecting thiocyanate with the naked eye based on color changes and its preparation method and application. It is capable of label-free testing, and the testing method is convenient and economical, has a good reusability rate, good anti-interference performance, and short response time. Suitable for real-time detection.

The invention discloses a coal and rock bore hydraulic fracture experimental apparatus, which comprises a sample device system, a confining pressure system, a fracture system, a sealing system and a monitoring system, wherein the sample device system comprises a sample cylinder, the bottom in the sample cylinder is provided with a screen drum of which the bottom is provided with a water outlet pipe, a sample is accommodated in the sample cylinder above the screen drum, and a simulated bore is formed in the sample; the confining pressure system comprises an air filling subsystem and a water filling subsystem; the fracture system comprises a fracture high-pressure water pump of which a water outlet is communicated with the simulated bore through a fracture pipeline; the sealing system comprises a sample cylinder sealing device and an experimental cylinder sealing device; and the monitoring system comprises an acoustic emission sensor arranged on an external wall of the sample cylinder. By the coal and rock bore hydraulic fracture experimental apparatus, a simulated experiment can be performed in a laboratory to investigate the change rules of crack initiation, expansion and extension in the hydraulic fracture process of a coal mine underground coal seam bore, and know a crack extending direction so as to guide field fracture; therefore, the fracture effect is greatly improved.

The invention discloses a method for detecting the quality of sorbic acid (potassium). The sorbic acid (potassium) to be tested is dried, and the difference between the light transmittance before drying and the light transmittance after drying is The thermal stability of sorbic acid, the smaller the thermal stability difference, the better the quality of sorbic acid (potassium) to be tested, the more stable the product, and the longer the shelf life.

The invention relates to the field of metallurgical casting, in particular to a phase field simulation method for predicting nano alpha twin crystal precipitation and microscopic structure evolution in titanium alloy in a local stress state, which comprises the following steps: S1, acquiring information such as Gibbs free energy density and interface energy of two phases and phase change strain tensors of different alpha variants in a beta-to-alpha solid phase change process of the titanium alloy; s2, establishing a phase field kinetic model, and solving a phase field control equation to obtain a sequence parameter result value; s3, in the phase transformation process under a certain supercooling degree, changing the stress load orientation and size, and obtaining different microstructure morphology information; and s4, performing visualization processing on corresponding microstructure evolution results under different input conditions, and clarifying an influence rule of a local stress state on a nano alpha twin crystal nucleation and evolution process. According to the method, the beta-to-alpha conversion process in the titanium alloy can be reproduced, and a visual prediction method is provided for the microstructure form and the evolution process of the titanium alloy under the thermal-mechanical coupling effect.

The invention provides a simulation method and system for a lumbar spondylolysis screw vertebral plate hook internal fixation system. The simulation method comprises the following steps: acquiring image data from the lumbar vertebra to the sacral vertebrae; segmenting and reconstructing the acquired image data to obtain a vertebral body three-dimensional model accompanied with lumbar spondylolysis; the three-dimensional model of the vertebral body is preprocessed; performing reverse materialization processing on the preprocessed cone three-dimensional model to obtain a cone curved surface model; according to the obtained vertebral body curved surface model, adding and assembling components of the lumbar spondylolysis screw vertebral plate hook internal fixation system to obtain an assembled curved surface model; performing grid division on each part to obtain a three-dimensional body grid of each part; carrying out material attribute assignment on the three-dimensional body mesh of the cone model by adopting an empirical formula; performing finite element analysis on the three-dimensional body mesh after material attribute assignment to obtain a simulation result; according to the invention, based on finite element analysis, more accurate analogue simulation of the lumbar spondylolysis screw vertebral plate hook internal fixation system is realized.

The invention provides a metal processing mold having automatic feeding and discharging functions. The metal processing mold comprises a lower pressing die, a screw rod, a bearing seat, a male die, awork clamping board, a servo motor, a rotating shaft, an electric telescopic rod and an electromagnet, wherein the screw rod is arranged in the lower pressing die; the bearing seat is mounted on the screw rod; the male die is fixed to the upper end surface of the bearing seat, and is arranged on the upper end surface of the lower pressing die; the work clamping board is assembled on the upper endsurface of the male die, and through the design, a fine adjustment function is realized; the servo motor is fixed to the front end of the lower pressing die; the rotating shaft is mounted at the upperend of the servo motor; the electric telescopic rod is fixed at the right end of the rotating shaft; the electromagnet is mounted at the right end of the electric telescopic rod; and the electric telescopic rod and the electromagnet are arranged above the lower pressing die, and through the design, automatic feeding and automatic discharging can be performed. According to the metal processing mold disclosed by the invention, the fine adjustment function is realized, the automatic feeding and discharging functions are realized, and the metal processing mold is high in practicality, good in safety and high in reliability.

Hydrogen peroxide eliminates the DTT interference method of nucleic acid constant temperature amplification and detection of mercury ions. The recognition sequence containing the mismatch site and the primer and template that triggers nucleic acid constant temperature amplification is mixed at a concentration of 100nM in equal volumes, and the recognition sequence is combined with the target to be detected. Mercury ions in mercury-containing solution can then form a stable hybrid structure that can be recognized by polymerase; mix the stable hybrid structure with hydrogen peroxide solution, amplification substrate, DNA polymerase KF, SYBR GreenⅠ, and amplification reaction buffer Under the action of polymerase, the amplification reaction is triggered to generate double-stranded DNA; SYBR Green I can specifically bind to double-stranded DNA molecules and emit fluorescent signals at the same time; the amplified sequence is characterized by melting curve and agarose gel electrophoresis , it is confirmed that hydrogen peroxide eliminates the interference of DTT, and the invention eliminates the interference of DTT on the detection of trace mercury ions, has good system compatibility, is easy to operate, and does not depend on complex equipment.

The invention provides a method for detecting protein based on a nanogold-DNA composite material, comprising the steps: preparing the nanogold-DNA composite, producing a nanogold-DNA-protein ternary composite, catalyzing nitrophenol and sodium borohydride with nanogold for reaction (a color development system), visually inspecting the period of time during which the color of reaction liquid changes from yellow to colorless (CCT value), drawing an LDA chart according to the CCT / CCT0 values of different protein, adding protein with given concentration to be detected into the color development system, calculating the CCT / CCT0 values of the protein to be detected, and identifying the protein to be detected with combination of the LDA chart. The method is established on the basis of a linear distinguishing and analysis technology and can successfully identify different protein only through visually inspecting and recording with a timer the period of time during which the color of the liquid changes, no expensive instrument is needed, the detection limit achieves 30nM, and a new technological means is provided for high flux quick detection of protein.

The invention discloses a refrigeration equipment rotor compressor load excitation recognition method under the time-varying working condition. The method is characterized in that vibration testing is performed on a refrigeration equipment rotor compressor exhaust muffler opening under the time-varying condition, an actual measurement acceleration response time history signal of the rotor compressor exhaust muffler opening is obtained, and an acceleration expected response matrix is constructed; a finite element method is used for constructing a refrigeration equipment rotor compressor virtual model machine model, the virtual model machine model is applied to analyzing acceleration response of an exhaust muffler opening of the refrigeration equipment rotor compressor under the effect of white noise loads, and a transfer function matrix is obtained. Back calculation is performed on the result of vibration testing of the rotor compressor exhaust muffler opening measured point under the time-varying condition to perform load excitation of the refrigeration equipment rotor compressor under the time-varying condition, and an iterative method is adopted for eliminating errors. Refrigeration equipment rotor compressor load excitation recognition analysis under the time-varying condition is achieved, and the basis is provided for the compressor-pipeline system vibration analysis and pipeline structure reliability design, estimation and the like.

The invention relates to real-time fluorescent quantitative PCR primers, a probe and a kit for detecting tupaia adenoviruses and belongs to the field of biotechnology. The primers are designed and synthesized according to the end 3' conserved sequence of the tupaia whole genome isolated and identified in this laboratory. The primers do not undergo a cross reaction with a tupaia source and common viruses belonging to other species. The end 5' of the probe is connected to a fluorescent reporting group and the end 3' of the probe is connected to a fluorescence quenching group. The kit produces accurate and reliable results, has good stability, good repeatability and high sensitivity, can fast and accurately quantify, has a wide detection range, can be used for epidemiological investigation caused by tupaia adenoviruses, provides the technical support for related basic research and has a wide application prospect.

The invention relates to a backflow type evaporation and crystallization device and a method for evaporating and crystallizing a mixed solvent. The device comprises a crystallization slot and an evaporation backflow slot, wherein the crystallization slot is communicated with a backflow liquid leading-in pipe of the evaporation backflow slot through a first backflow condenser pipe; a detection port is formed in the bottom of the evaporation backflow slot; a U-shaped pipe is fixedly arranged in the evaporation backflow slot; one end opening of the U-shaped pipe is in a hopper shape and directly faces to the outlet of the backflow liquid leading-in pipe; the other end of the U-shaped pipe is in a convergent type and has an end opening lower than the hopper shaped end opening; the evaporation backflow slot is communicated with a backflow pipe through a second backflow condenser pipe; the backflow pipe is communicated with the crystallization liquid inlet of the crystallization slot through a preheater. By the backflow type evaporation and crystallization device disclosed by the invention, the temperature of the evaporation backflow slot can be adjusted so as to control the backflow rate; the proportion reduction of easily volatile components of the solvent in the crystallization slot is effectively compensated, so that the component proportion of the mixed solvent in the crystallization slot is not changed; the backflow type evaporation and crystallization device is convenient in use, high in working efficiency and less in raw material waste.

DNA structure probes based on nucleic acid constant temperature amplification technology to detect mercury ions, including nucleic acid sequences of three DNA structure probes that contain mismatch sites and can form a triple hybridization structure through nucleic acid hybridization. The triple hybridization structure is characterized by being triggered by mercury ions The DNA probe structure is formed by hybridization, which spatially separates the mercury ion binding region and the polymerase recognition region, and uses the rapid polymerization of the polymerase to obtain a stable structure for sustainable polymerization before the biothiol molecule destroys the interaction between mercury ions and thymine , cleverly avoided the interference of biothiol molecules. This method can effectively eliminate the loss of trace mercury ions due to the interference of biothiol molecules when the nucleic acid constant temperature method is used to detect, effectively improve the detection efficiency of trace mercury ions without introducing any reagents, and further improve the detection efficiency of nucleic acid. Application of isothermal amplification technology in the detection of mercury ions.

The invention belongs to the technical field of reactor simulation experiments, and particularly relates to an experimental model for a sodium-cooled pool type fast reactor natural circulation experiment. The experimental model comprises a cylindrical shell formed by sequentially connecting an upper sealing head, an upper cylinder body, a lower cylinder body and a lower sealing head from top to bottom in a sealed mode, the upper sealing head and the upper cylinder body form a hot pool, and the lower cylinder body and the lower sealing head form a cold pool. The experimental model also comprises a simulated reactor core located in the center of the shell, a central measuring column simulation part located above the simulated reactor core, a large grid plate header simulation part located below the simulated reactor core, a DR type heat exchanger simulation part arranged in the hot pool, a DL type heat exchanger simulation part arranged in the cold pool, and an M-type heat exchanger simulation part arranged between the hot pool and the cold pool in a penetrating manner. Working media in the cold pool can be powered by an external circulating pump to sequentially enter the large grid plate header simulation part and the simulation reactor core to enter the hot pool, and then return to the cold pool again after exchanging heat with the M-type heat exchanger simulation part.