Method for building model of piglets infected by haemophilus parasuis (HPS)

Abstract

The invention discloses a method for building a model of piglets infected by haemophilus parasuis (HPS) and relates to the field of animal model building. The method includes that a plurality of colostrum-deprived (CD) piglets are raised to 18-25 days old and uniformly divided into an infected group A and a control group B, the fact that the CD piglets are free from HPS pathogens, APP pathogens or APP antibodies is determined, then the piglets are raised to 40-70 days old, HPS strains are inoculated to tracheas of all CD piglets in the infected group A, culture media are injected to the tracheas of all CD piglets in the control group B to obtain a blank group B', a toxic group A' and the blank group B' are observed and detected, total deoxyribose nucleic acid (DNA) of lung tissue of the toxic group A' and the blank group B' is extracted, and the lung tissue of the blank group B' is free from the HPS pathogens while the HPS pathogens are determined to exist in the lung tissue of the toxic group A'. By means of the method for building the model of the infected piglets by HPS, experiment costs can be reduced, the modeling process is simple and convenient, the result accuracy is high, and the method can meet relevant requirements of animal welfare.

Description

technical field [0001] The invention relates to the field of animal model construction, in particular to a method for constructing a piglet model infected with Haemophilus parasuis. Background technique [0002] HPS (Haemophilus parasuis, Haemophilus parasuis) is a Gram-negative bacillus that colonizes the upper respiratory tract of pigs. The virulent strains of HPS can cause severe systemic inflammatory diseases in pigs after invading pigs. Clinical manifestations Glaser's disease ( Disease), namely fibrinoid pericarditis, polyarthritis and meningitis; some infected pigs also showed acute pneumonia and acute sepsis. [0003] From the end of the last century to the present, HPS infection has caused serious losses to the pig industry in various countries around the world. HPS has become one of the most important bacterial pathogens in pig farms, showing a national trend in my country. [0004] There are no effective measures to prevent HPS infection at this stage. Usually...

AI Technical Summary

Problems solved by technology

[0014] (1) When feeding CD piglets, first use bovine colostrum to feed for 3 days. Generally speaking, it is inconvenient to obtain bovine colostrum and needs to be sterilized, which not only increases the cost of the experiment, but also may increase the risk of bovine enteritis virus. Risk of diarrhea in CD piglets, higher mortality in CD piglets

[0015] (2) The existing model was used to challenge 21-day-old CD piglets. Since the internal organs of 21-day-old CD piglets are not fully developed, the relevant experimental results may not fully reflect the symptoms of pigs infected with HPS, and the accuracy is low. ; At the same time, using CD piglets close to the weaning age (15-21 days) on production for experiments is not in line with animal welfare measures

[0016] (3) The survival rate of existing CD piglets before infection is only 50% to 78.9%, the survival rate is low, and the experimental cost is high

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