Method for identifying natural crocodile-skin leather

Abstract

The invention provides a method for identifying natural crocodile-skin leather and particularly relates to a primer for identifying natural crocodile-skin leather with the real-time fluorescence PCR technology, a probe and a concrete detection method. High sensitivity and specificity are achieved, and whether leather is natural crocodile-skin leather or not can be quickly and accurately identified with the combination of the identifying method.

Description

Technical field: [0001] The invention belongs to the field of leather detection, and specifically relates to a primer and a probe for real-time fluorescent PCR detection and a method for identifying natural crocodile leather by using the primer probe. Background technique [0002] Leather is denatured and non-perishable animal skin obtained through physical and chemical processing such as depilation and tanning. The price of leather is relatively high, and the factors affecting its price are not only the processing technology, but also the type of leather or fur used. Among them, crocodile leather can be called the platinum in leather, and is famous for its luxury and rarity. This is not only because of the scarcity of crocodiles, but also because of the slow growth rate of crocodiles and the high cost of breeding, and the usable crocodile skin is limited to the long and narrow part of the crocodile's abdomen. Crocodile leather has a natural gradient grid pattern and is du...

AI Technical Summary

Problems solved by technology

Infrared spectroscopy uses the difference in amino acid types and composition ratios in leather to irradiate the surface of leather samples with continuous wavelength infrared light, causing molecular vibrations and transitions between energy levels, and obtaining different spectra in the infrared absorption spectrum, but currently There are only common detection methods for pig, cattle, sheep, horse and other leathers

In addition, more chemical reagents will be added in the process of leather processing, and the coating on the leather surface will greatly interfere with the infrared spectrum, so this method is still in the exploratory stage in the identification of finished leather

[0006] The DNA identification method is to extract the DNA of animal sources in leather products, design primers for the specific gene sequence of the species, obtain the gene sequence of the target species through PCR amplification of the specific gene, and use real-time fluorescent PCR method or sequencing comparison according to the amplification product. Equivalent molecular biotechnology is used to determine the animal-derived components of leather, but there is no report on the identification of natural crocodile leather using DNA identification methods

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