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Mixed fermentation strain used for fermentation of biological cellulose

A technology of mixing fermentation bacteria and biocellulose, which is applied in microorganism-based methods, fermentation, microorganisms, etc., and can solve problems such as low fermentation level

Pending Publication Date: 2016-11-23
HAINAN YEGUO FOODS
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The conversion rate of reducing sugar is only below 20%, and the fermentation level is very low

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] A mixed fermentation agent for biological cellulose fermentation, comprising Gluconacetobacter xylinum, Zymomonas mobilis, Bifidobacterium and / or Lactobacillus acidophilus.

[0020] The Bifidobacterium and Lactobacillus acidophilus can contain only one of them, or both.

[0021] Bacteria preparation steps:

[0022] 1. Activation of strains

[0023] Gluconacetoacetobacter xylinum, Zymomonas mobilis, Bifidobacterium and Lactobacillus acidophilus were respectively cultured and activated on the activation medium;

[0024] 2. Seed liquid culture

[0025] Gluconacetoacetobacter xylinum, Zymomonas mobilis, Bifidobacterium, and Lactobacillus acidophilus were respectively inoculated into the seed medium for expansion, and cultured to their logarithmic growth phase;

[0026] 3. Inoculation and fermentation

[0027] The seed solution of Gluconacetoacetobacter xylinum, Zymomonas mobilis, and Bifidobacterium and Lactobacillus acidophilus is in a volume ratio of 17:11:5 (wherein ...

Embodiment 2

[0030] Others are the same as in Example 1, and the mixed fermentation agent is 15:8:6 Gluconacetoacetobacter xylinum, Zymomonas mobilis, and Bifidobacterium.

Embodiment 3

[0032] Others are the same as in Example 1, and the mixed fermentation agent is 20:15:8 of Gluconoacetobacter xylinum, Zymomonas mobilis, and Lactobacillus acidophilus.

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Abstract

The invention relates to a mixed fermentation strain used for fermentation of biological cellulose, wherein the mixed fermentation strain comprises gluconacetobacter xylinus, zymomonas mobilis, bifidobacterium and / or lactobacillus acidophilus. The ratio of the gluconacetobacter xylinus to the zymomonas mobilis to the bifidobacterium and / or lactobacillus acidophilus is (10-20):(7-15):(3-8), preferentially 17:11:5. The strains are subjected to activation culture before use. The ratio of the strains is the volume ratio of a seed solution obtained through activation and then expanding culture. The mixed strains mutually benefit and form a symbiotic situation. The time for the formation of a biological cellulose gel membrane is prolonged, the conversion rate of a carbon source to a reducing sugar is improved, and the capability of producing the membrane through the fermentation of the biological cellulose is greatly improved.

Description

[0001] technical field [0002] The invention relates to the technical field of biological fermentation, in particular to a mixed fermentation bacterial agent for biological cellulose fermentation and a method for preparing biological cellulose by fermentation thereof. Background technique [0003] Biocellulose, also known as bacterial cellulose (BC), is a cellulose product made by fermentation of microorganisms of the genus Acetobacter, such as Acetobacter xylinum. At present, waste materials, such as coconut water and pineapple peel juice, are used as raw materials, so the cost is low. The obtained product contains more than 95% moisture, so it is also called biocellulose gel. The preparation method of biocellulose gel is known in the art, as disclosed in CN101671708, CN1840677A, and CN101319242A. Compared with plant cellulose, biocellulose has no associated products such as lignin, pectin and hemicellulose, and has high crystallinity (up to 95%, plant cellulose is 65%) ...

Claims

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Application Information

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IPC IPC(8): C12N1/20C12P39/00C12P19/04C12R1/23C12R1/01
Inventor 钟春燕钟宇光
Owner HAINAN YEGUO FOODS
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