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High glucose culture medium for fermentation of biological cellulose

A high-sugar medium and bio-cellulose technology, applied in the direction of microorganism-based methods, fermentation, microorganisms, etc., can solve the problem of low fermentation level and achieve the effect of improving film production capacity

Pending Publication Date: 2016-11-23
HAINAN YEGUO FOODS
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The conversion rate of reducing sugar is only below 20%, and the fermentation level is very low

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] A high-sugar medium for biocellulose fermentation, containing the following substances (by weight):

[0020] Glucose 15%, yeast extract 0.5%, peptone 0.5%, citric acid 0.1%, Na 2 HPO 4 ·H 2 O 0.2%, KH 2 PO 4 0.1%, MgSO 4 ·7H 2 O 0.025%, pH5.8.

Embodiment 2

[0021] Embodiment 2: all the other are identical with embodiment 1, and carbon source is fructose 18%.

Embodiment 3

[0022] Embodiment 3: all the other are the same as embodiment 1, and the carbon source is 20% sucrose.

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PUM

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Abstract

The invention relates a special high glucose culture medium for fermentation of biological cellulose, wherein the high glucose culture medium contains 15-25wt% of reducing sugar. When the high glucose culture medium is used for fermentation of the biological cellulose, an adopted fermentation strain is a mixed strain and comprises gluconacetobacter xylinus, zymomonas mobilis, bifidobacterium and / or lactobacillus acidophilus. After the three strains are used, the content of the reducing sugar in the culture medium is greatly improved to about 15-25wt% from the commonly less than 10wt%, the time for the formation of a biological cellulose gel membrane is prolonged, the conversion rate of a carbon source to the reducing sugar is improved, and the capability of producing the membrane through the fermentation of the biological cellulose is greatly improved.

Description

[0001] technical field [0002] The invention relates to the technical field of biological fermentation, in particular to a high-sugar medium for biological cellulose fermentation and a method for preparing biological cellulose by fermentation thereof. Background technique [0003] Biocellulose, also known as bacterial cellulose (BC), is a cellulose product made by fermentation of microorganisms of the genus Acetobacter, such as Acetobacter xylinum. At present, waste materials, such as coconut water and pineapple peel juice, are used as raw materials, so the cost is low. The obtained product contains more than 95% moisture, so it is also called biocellulose gel. The preparation method of biocellulose gel is known in the art, as disclosed in CN101671708, CN1840677A, and CN101319242A. Compared with plant cellulose, biocellulose has no associated products such as lignin, pectin and hemicellulose, and has high crystallinity (up to 95%, plant cellulose is 65%) and high degree o...

Claims

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Application Information

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IPC IPC(8): C12P39/00C12P19/04C12R1/23C12R1/01
Inventor 钟春燕钟宇光
Owner HAINAN YEGUO FOODS
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