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High-survival-rate transplanting method for transgenic cotton regenerated plants

A regeneration plant and transgene technology, applied in the field of transgenic cotton, can solve the problems of easy rot of grafted seedlings and rootstocks, achieve the effect of enhancing growth and root density, increasing the link of tap water to harden seedlings, and the operation method is simple and easy.

Inactive Publication Date: 2016-12-07
NANJING AGRICULTURAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

At the same time, grafted seedlings and rootstocks are prone to rot in the wrapped environment, especially in the high temperature season of summer.

Method used

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  • High-survival-rate transplanting method for transgenic cotton regenerated plants
  • High-survival-rate transplanting method for transgenic cotton regenerated plants
  • High-survival-rate transplanting method for transgenic cotton regenerated plants

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Experimental program
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Effect test

Embodiment 1

[0033] (1) Transgenic cotton regenerated plant rooting seedlings grow to the bottle cap of the triangular flask in the medium, the medium at the root of the regenerated plant is washed, transplanted into an empty triangular flask, and an appropriate amount (submerging the root of the regenerated plant) Tap water (such as figure 1 Hydroponic seedling hardening was carried out for transgenic cotton regenerated strains that had just been transplanted into tap water bottles from the culture medium.

[0034] (2) Cultivate in an incubator with a humidity of 60%-70%, a temperature of 27°C-29°C, and light (day / night: 16h / 8h, 12000-15000Lx). The tap water in the triangular flask was replaced every 2 to 3 days, and cultivated for 15 days until the transgenic regenerated plants were robust and grew more roots ( figure 2 It is the transgenic cotton seedling after hydroponic hardening for 15 days).

[0035] (3) Transgenic regenerated strains after hydroponic seedling hardening are trans...

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Abstract

The invention discloses a high-survival-rate transplanting method for transgenic cotton regenerated plants. The method includes the steps that rooting seedlings of transgenic cotton regenerated plants are taken out of a culture medium, the culture medium at the roots of the regenerated plants is washed away, the regenerated plants are transferred into a new cultivation container, water is added into the cultivation container to immerse the roots of the regenerated plants for water cultivation seedling hardening, and transgenic plants growing vigorously after water cultivation seedling hardening are transplanted into a medium or soil and cultured until cotton boll opening and maturing. According to the method, in the process of transplanting the rooting seedlings of the transgenic cotton regenerated plants into the medium / soil from the culture medium, the water cultivation seedling hardening step is carried out, the growth vigor and root density of the transplanted seedlings can be improved, and vigorous seedlings can be cultured; the problem that regenerated plants are directly transplanted into a medium / soil from a culture medium, and consequently roots and stems are likely to rot is avoided. By means of the method, the survival rate of the transplanted seedlings reaches 98% or above; besides, the operation method is simple and easy to implement.

Description

technical field [0001] The invention relates to the technical field of transgenic cotton, in particular to a method for transplanting regenerated transgenic cotton plants with high survival rate. Background technique [0002] Traditional cotton Agrobacterium-mediated transformation studies use hypocotyls as explants, and their transformation and regeneration undergo somatic embryogenesis pathways, high-frequency transformation regeneration systems for regenerated plants, etc. Restricted by many factors such as long transformation cycle, low embryo formation rate, prone to deformed embryos, low regeneration ratio of somatic embryo plants and lack of stem elongation, the whole process of obtaining transgenic regenerated plants is relatively long. Generally, it takes 8 to 12 months from the start of Agrobacterium co-cultivation to obtain complete regenerated seedlings. On this basis, the transgenic regenerated seedlings whose target gene has been successfully introduced into t...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01G1/00A01G31/00
CPCA01G22/00A01G31/00
Inventor 郭旺珍周雪松
Owner NANJING AGRICULTURAL UNIVERSITY
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