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Compound bacterial agent for promoting the breeding of potato test-tube seedlings and its preparation and use

A technology of composite bacterial agents and test-tube seedlings, which is applied in botany equipment and methods, biochemical equipment and methods, and methods based on microorganisms, can solve problems such as inability to obtain nutrients, prolong the breeding cycle, and ineffective transportation of nutrients, and achieve Improve the reproduction quantity and reproduction speed, promote the survival rate and growth, and improve the effect of transplanting seedling survival rate

Active Publication Date: 2022-08-02
INNER MONGOLIA AGRICULTURAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, repeated subcultures in the process of in vitro shoot apex culture or stem segment expansion often lead to poor development of test-tube plantlets such as slow growth, thin stems, and underdeveloped roots. Plants do not grow, leaves fall off or even die, which eventually leads to low seed potato yield, poor quality, and increased production costs
This is mainly because the root system of test-tube seedlings is not well developed, the stems are thin, and the seedlings are weak, which cannot compensate for the rapid loss of water from the stomata, cannot effectively obtain nutrients from the soil, and cannot effectively transport nutrients, etc.

Method used

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  • Compound bacterial agent for promoting the breeding of potato test-tube seedlings and its preparation and use
  • Compound bacterial agent for promoting the breeding of potato test-tube seedlings and its preparation and use
  • Compound bacterial agent for promoting the breeding of potato test-tube seedlings and its preparation and use

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0040] Example 1: Isolation and identification of bacterial species

[0041] The roots of potato seedlings in the field were collected, and the root-related (root surface and endogenous) nitrogen fixation, phosphorus solution, phytohormone production, polysaccharide production, siderophore production and ACC deaminase production were separated by coating and streaking technology. Strain function; using the isolated and purified strain genomic DNA as a template, using 1492R and 27F as primers, PCR amplification of the 16S rRNA gene of the strain, sequencing the amplified product, and the obtained sequence is classified by online EzTaxon (16S rRNA gene sequence similarity) The 16S rRNA gene sequences of the obtained 180 bacterial strains were 98% to 100% similar to the type species. The results are shown in Table 1, which is an analysis of the 16S rRNA gene similarity of the strains with strong plant growth-promoting function.

[0042] Table 1:

[0043]

Embodiment 2

[0044] Example 2: The effect of single bacteria on the growth of potato test-tube seedlings

[0045] Activation culture of strains with strong plant growth-promoting ability: draw 100 μL of glycerol bacterial preservation solution and spread it on the modified R2A solid medium, place it at 25°C, and pick out a single colony with typical colony characteristics and rapid growth and place the modified R2A Liquid medium, cultured at 25 °C; centrifuged at 8000 g centrifugal force when cultured to the middle and late logarithmic phase, and centrifuged at K 2 HPO 4 -KH 2 PO 4 Buffer (pH7.2) was washed 3 times and then resuspended with the same buffer until the effective viable bacteria concentration was 10. 12 CFU / mL.

[0046] Mix each bacterial suspension with MS medium whose temperature has dropped to about 50℃~55℃ after high temperature and high pressure sterilization, shake gently to mix evenly, and pour it into the culture bottle quickly; , in 22 ℃ ~ 26 ℃ light culture. Se...

Embodiment 3

[0053] Example 3: Preparation of compound bacterial agent

[0054] When inoculated alone, L10, FN14 and B28 had the highest increase in root length, stem diameter and node number of potato test-tube seedlings respectively, while other bacteria had no obvious improvement effect, and even more inhibited. Therefore, L10, FN14 and B28 were selected as the compound bacterial strains. The results of the plate confrontation experiment showed that there was no obvious mutual antagonism among the three strains, and they could be used for compounding. The promotion effect after compounding with different proportions of effective viable bacteria (Table 3) was used to determine the initial optimal number of effective viable bacteria for different strains. Table 3 shows the effect of different numbers of effective viable bacteria on growth in the compound inoculum.

[0055] table 3:

[0056]

[0057]

[0058] Note: P<0.05.

[0059] When L10, FN14 and B28 were single inoculated, t...

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Abstract

The invention provides a compound inoculant for promoting the breeding of potato test-tube seedlings and a preparation and use method thereof. The functional strains of the compound bacterial agent include Fictibacillus halophilus, Bacillus gobiensis and Hymenobcter jeollabukensis, and the ratio of the effective viable bacteria of the three strains is (1-3):(2-5):(2-5). The compound bacterial agent of the invention is inoculated into the potato test-tube seedlings, which can significantly increase the number of test-tube seedlings, the root length and the stem thickness by about 60% to 80%, significantly improve the reproductive quantity and speed of the test-tube seedlings, and significantly promote the transplanting of seedlings. The survival rate and growth of seedlings significantly promote the growth and development of seedlings after transplanting, increase the number of tubers, increase the volume and weight of a single tuber; enhance the resistance of seedlings to soil-borne diseases, and have resistance to soil-borne diseases such as late blight and scab. Adversity potential such as disease and saline-alkali stress.

Description

technical field [0001] The invention belongs to the technical field of microbial resource utilization and ecological agriculture, relates to a compound inoculant for promoting the breeding of potato test-tube seedlings and a preparation and use method thereof, and is suitable for the breeding of potato test-tube seedlings. Background technique [0002] Potato is a combined food and vegetable crop in the world, and the fourth largest staple crop after rice, wheat and corn. In potato production, disease is the most important factor affecting the yield and quality of potato, and many diseases can be carried and spread through seed potatoes (including tuber cuttings). The industrialization and large-scale breeding of seed potatoes through test-tube seedlings can prevent seed potatoes from carrying diseases and viruses, reduce the spread of diseases, and promote the development of the potato industry. [0003] Potato in vitro seedlings (fast-propagating seedlings) are produced b...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20A01H4/00C12R1/07C12R1/01
CPCC12N1/20A01H4/008A01H4/001C12R2001/07C12N1/205C12R2001/01
Inventor 唐凯李蘅路晓培杨杉杉冯福应袁博云欣悦郭慧玲
Owner INNER MONGOLIA AGRICULTURAL UNIVERSITY
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