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Method capable of increasing transplanting survival rate of nicotiana benthamiana tissue culture seedlings

A technology of transplanting survival rate and tissue culture seedlings, which is applied in the field of plant breeding and cultivation, can solve the problems of long transplanting time, damage to tissue culture seedlings, and low survival rate of transplanting, so as to reduce working steps, speed up the transplanting process, The effect of improving the survival rate of transplanting

Active Publication Date: 2020-01-14
RES INST OF SUBTROPICAL FORESTRY CHINESE ACAD OF FORESTRY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] At present, in the process of training and transplanting tobacco tissue cultured seedlings, it is often necessary to carry out complicated sterilization work on the tissue cultured seedlings, such as soaking in carbendazim solution, washing with sterile water repeatedly, etc. The protection of the tissue culture seedlings will be damaged to varying degrees after sterilization, resulting in a low survival rate of transplanting and affecting the progress of the experiment.
At the same time, the process of sterilization and training seedlings is cumbersome, which makes the transplanting time too long and affects the growth process of tissue culture seedlings

Method used

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  • Method capable of increasing transplanting survival rate of nicotiana benthamiana tissue culture seedlings
  • Method capable of increasing transplanting survival rate of nicotiana benthamiana tissue culture seedlings
  • Method capable of increasing transplanting survival rate of nicotiana benthamiana tissue culture seedlings

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Effect test

Embodiment 1

[0033] The N. benthamiana tissue culture seedlings that took root 2-3 weeks (root length 2-3cm) and grew robustly were placed in an artificial incubator and carried out a transitional culture. The time of the first transitional culture was 12 days. The ambient temperature of the incubator The temperature is 21°C, the humidity is 70% RH, and the light is 6000Lx. Then remove it from the artificial incubator, and keep the soil moist in an indoor lighting and ventilation place, and carry out secondary transitional cultivation for 7 days. After two transitional cultivations, transplant the vigorously grown tissue-cultured seedlings to the normal open-air environment, perform watering and fertilization management as usual, and then count the survival rate of transplanting.

Embodiment 2

[0035] The N. benthamiana tissue culture seedlings that took root 2-3 weeks (root length 2-3cm) and grew robustly were placed in an artificial incubator and carried out a transitional culture. The time for the first transitional culture was 10 days. The ambient temperature of the incubator The temperature is 21°C, the humidity is 70% RH, and the light is 6000Lx. Then remove it from the artificial incubator, and keep the soil moist in an indoor lighting and ventilation place, and carry out secondary transitional cultivation for 7 days. After two transitional cultivations, transplant the vigorously grown tissue-cultured seedlings to the normal open-air environment, perform watering and fertilization management as usual, and then count the survival rate of transplanting.

Embodiment 3

[0037] The N. benthamiana tissue culture seedlings that took root 2-3 weeks (root length 2-3cm) and grew robustly were placed in an artificial incubator and carried out a transitional culture. The time for the first transitional culture was 10 days. The ambient temperature of the incubator The temperature is 21°C, the humidity is 80% RH, and the light is 6000Lx. Then remove it from the artificial incubator, and keep the soil moist in an indoor lighting and ventilation place, and carry out secondary transitional cultivation for 5 days. After two transitional cultivations, transplant the vigorously grown tissue-cultured seedlings to the normal open-air environment, perform watering and fertilization management as usual, and then count the survival rate of transplanting.

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Abstract

The invention relates to a method capable of increasing transplanting survival rate of nicotiana benthamiana tissue culture seedlings. The method is characterized by including following steps: takingnicotiana benthamiana tissue culture seedlings rooting for 2-3 weeks out of a tissue culture bottle, and using flowing water to flush the tissue culture seedlings to remove a root culture medium; sufficiently watering planting soil, and putting the planting soil into a culture basin; transplanting the tissue culture seedlings after being treated into the planting soil of the culture basin, and transferring the culture basin into an artificial culture box or an artificial weather chamber for first-time transition culture for 7-10d; moving the tissue culture seedlings after first-time transitionculture out of the artificial culture box or the artificial weather chamber, putting at an indoor daylighting ventilated position, maintaining soil wet, and performing second-time transition culturefor 3-5d; after second-time transition culture, moving tissue culture seedlings growing robustly into a normal outdoor environment, and performing conventional watering and fertilizing management. Transition culture of the tissue culture seedlings is realized through the artificial culture box, indoor transition and direct outdoor transplanting, so that working steps can be reduced, transplantingprocess is accelerated, and transplanting survival rate is increased remarkably.

Description

technical field [0001] The invention belongs to the technical field of plant breeding and cultivation, and in particular relates to an outdoor transplanting method for improving the transplanting survival rate of Nicotiana benthamiana tissue culture seedlings. Background technique [0002] Nicotiana benthamiana is an annual or perennial plant of the genus Nicotiana in the family Solanaceae, and Nicotiana benthamiana is an important model plant in current plant genetic engineering breeding. First, Nicotiana benthamiana is the most widely used experimental host in plant virology, mainly due to the wide variety of plant viruses that can successfully infect tobacco. Furthermore, N. benthamiana is susceptible to a variety of other plant pathogens (e.g., bacteria, oomycetes, fungi, etc.), making this species a cornerstone in the study of host pathogens, especially in the context of innate immunity and defense signaling. Most importantly, N. benthamiana has rapidly become popular ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01G31/00A01G24/28A01G24/15A01H4/00
CPCA01G31/00A01G24/28A01G24/15A01H4/00
Inventor 李纪元姜丽娜童冉范正琪殷恒福李辛雷
Owner RES INST OF SUBTROPICAL FORESTRY CHINESE ACAD OF FORESTRY
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